Nucleotide sequence of the leader RNA of the New Jersey serotype of vesicular stomatitis virus

Colonno, Richard J.; Banerjee, Amiya K.

doi:10.1093/nar/5.11.4165

Cited by 33 publications

(16 citation statements)

References 19 publications

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“…Also, an adenylic acid and uridylic acid difference was seen at position 31, which may be due to strain difference between the virus used here and that used by Colonno and Banerjee (4).…”

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confidence: 76%

“…The gene order on the virion is 3'-N-NS-M-G-L-5' (1, 2). The nucleotide sequences of the leader RNAs of both VSV Indiana and New Jersey serotypes have been determined by the analysis of partial ribonuclease digestion products after in vitro 32p labeling of the 5' end with polynucleotide kinase or of the 3' end with RNA ligase (3,4). Confirmation that the sequence of the VSV Indiana leader RNA is complementary to the 3'-terminal sequence of the virus RNA, at least up to 17 bases from the 3' terminus, has been obtained by sequence determination of virus RNA labeled at the 3' end with RNA ligase (5).…”

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confidence: 99%

“…In most cases this phenomenon varied among experiments so that ambiguities in the sequence could be eliminated by comparing several gels. From the published sequences of the 3' ends of VSV Indiana (3,5) and New Jersey (4), it can be predicted that the first base (3,4) and VSV Indiana N mRNA rib~osome binding site (6). Bases that are different in the RNAs from the two serotypes are underlined and asterisks indicate bases that were regularly ambiguous.…”

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confidence: 99%

“…to be detected by using dT8AC as a primer and [32P]dATP as label should be the fourth from the 3' end. In fact the first base that could clearly be seen as the start of a readable sequence on the cDNA gels was the seventh from the 3' end, according to published sequence data (3)(4)(5).…”

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confidence: 99%

“…4 together with the predicted sequence of the virus RNAs. Included for comparison are published sequences for the leader RNAs of both viruses (3,4) and the sequence of the N mRNA protected from ribonuclease digestion by binding to ribosomes (6). The first part of the sequence of VSV Indiana RNA was found with one exception to be compatible with the sequence of its transcript, the leader RNA as determined by Colonno and Banerjee (3).…”

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confidence: 99%

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Sequences of vesicular stomatitis virus RNA in the region coding for leader RNA, N protein mRNA, and their junction.

Rowlands¹

1979

Proc. Natl. Acad. Sci. U.S.A.

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The RNAs extracted from purified preparations of the Indiana and New Jersey serotypes of vesicular stomatitis virus were polyadenylylated in vitro by using polynucleotide phosphorylase and sequence determination was carried out by the dideoxynucleotide method using reverse transcriptase and dTSAC primer. On both virus RNAs a short stretch of adenylic acid residues is present between the regions coding for the leader and N protein mRNAs. Other features of the RNA sequences of the two viruses are compared to each other and to published data.Vesicular stomatitis virus (VSV) RNA is a molecule of molecular weight 4 X 106 and has negative polarity. Expression of the genetic information encoded in the virion RNA proceeds via sequential primary transcription from the 3' end of virion RNA of a short nonpolyadenylylated leader sequence followed by polyadenylylated mRNAs corresponding to the five virusspecific proteins (N, NS, M, G, and L) induced in cells during infection. The gene order on the virion is 3'-N-NS-M-G-L-5' (1, 2). The nucleotide sequences of the leader RNAs of both VSV Indiana and New Jersey serotypes have been determined by the analysis of partial ribonuclease digestion products after in vitro 32p labeling of the 5' end with polynucleotide kinase or of the 3' end with RNA ligase (3, 4). Confirmation that the sequence of the VSV Indiana leader RNA is complementary to the 3'-terminal sequence of the virus RNA, at least up to 17 bases from the 3' terminus, has been obtained by sequence determination of virus RNA labeled at the 3' end with RNA ligase (5). The sequence of nucleotides in the ribosome binding sites of three of the VSV Indiana mRNAs, including N mRNA has been determined (6). Because the protected sequences of N and M mRNAs contain the cap, they must include data available spanning the region between the end of the leader RNA and the start of the first mRNA sequence.Recently, application of the dideoxynucleotide method of sequence determination by introducing base-specific stops in cDNA (7) has been successfully employed to obtain extensive sequence data of the regions adjacent to the poly(A) tract in two naturally polyadenylylated virus RNAs, encephalomyocarditis virus RNA (8) and poliovirus RNA (9), and of globin mRNA (10). This paper reports extended sequence information on the 3' end of VSV RNA, including the leader/N mRNA junction, obtained by the dideoxynucleotide method, using as template RNA that had been polyadenylylated in vitro with polynucleotide phosphorylase. MATERIALS AND METHODSViruses and RNAs. VSV of serotypes Indiana (MuddSummers strain) and New Jersey (Ogden) was grown in baby hamster kidney cells (BHK-21) infected with cloned virus stocks and incubated with Eagle's minimal essential medium supplemented with 7% calf serum. The virus was purified as described by Doyle and Holland (11) and the RNA was extracted as described by Perrault (12) and purified by sedimentation through a 5-25% sucrose gradient in 0.2 M NaCl/50 mM Tris-HCI/5 mM EDTA/0.1% Sarkosyl at pH 7.4 (NTE/Sa...

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“…Also, an adenylic acid and uridylic acid difference was seen at position 31, which may be due to strain difference between the virus used here and that used by Colonno and Banerjee (4).…”

mentioning

confidence: 76%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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