Nucleotide sequence of the histone H3-encoding gene from the scleractinian coral Acropora formosa (cnidaria: scleractinia)

Miller, David J.; McMillan, J.; Miles, A; Lohuis, Michael ten; Mahony, Timothy J.

doi:10.1016/0378-1119(90)90243-k

Cited by 10 publications

(4 citation statements)

References 7 publications

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“…The sets of genes differentially regulated by MDP and poly I:C are largely discrete: only 15 genes were differentially expressed in response to both treatments (Figure 1), with few known genes responding to challenge with MDP and poly I:C in the same way. Amongst these, Cluster001881, which encodes the core histone H3 [29], was strongly up-regulated relative to controls. Cluster026407, which encodes an HSP12/ORP150-related chaperone, was the most strongly up-regulated gene under both treatments.…”

Section: Resultsmentioning

confidence: 99%

The acute transcriptional response of the coral Acropora millepora to immune challenge: expression of GiMAP/IAN genes links the innate immune responses of corals with those of mammals and plants

et al. 2013

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BackgroundAs a step towards understanding coral immunity we present the first whole transcriptome analysis of the acute responses of Acropora millepora to challenge with the bacterial cell wall derivative MDP and the viral mimic poly I:C, defined immunogens provoking distinct but well characterised responses in higher animals.ResultsThese experiments reveal similarities with the responses both of arthropods and mammals, as well as coral-specific effects. The most surprising finding was that MDP specifically induced three members of the GiMAP gene family, which has been implicated in immunity in mammals but is absent from Drosophila and Caenorhabditis. Like their mammalian homologs, GiMAP genes are arranged in a tandem cluster in the coral genome.ConclusionsA phylogenomic survey of this gene family implies ancient origins, multiple independent losses and lineage-specific expansions during animal evolution. Whilst functional convergence cannot be ruled out, GiMAP expression in corals may reflect an ancestral role in immunity, perhaps in phagolysosomal processing.

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Section: Resultsmentioning

confidence: 99%

The acute transcriptional response of the coral Acropora millepora to immune challenge: expression of GiMAP/IAN genes links the innate immune responses of corals with those of mammals and plants

et al. 2013

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“…The presence of such an intronless gene was correlated to the relative low position of cnidarians in the evolutionary lineage. In spite of the fact that there are similar uninterrupted genes from different classes of the phylum cnidaria [23–25], it is not a general rule to cnidarian gene organization (see later). It may, nevertheless, reflect on the ability of these predatory organisms to evolve a selected set of genes for quick response to external stimuli (as illustrated by Anderluh et al [22]).…”

Section: Discussionmentioning

confidence: 99%

Genomic structure of a potassium channel toxin from Heteractis magnifica¹

1997

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We provide information on the gene encoding the K + channel toxin, HmK, of the sea anemone Heteractis magnified. A series of DNA amplifications by PCR, which included the amplification of the 5'-untranslated region of the gene, showed that an intron of 402 nucleotides separated the sequence that encodes the matured toxin from the signal peptide sequence. A second 264 nucleotide intron interrupted the 5'-untranslated region of the previously reported HmK cDNA. Two possible transcription-initiation sites were identified by primer extension analysis. Corresponding TATA-box consensus sequences, characteristic of a promoter region, were also located from PCR products of uncloned libraries of adaptor-ligated genomic DNA fragments. The coding region for matured HmK is intronless. The same is also true for other sea anemone toxins reported thus far. More notably, a similar intron-exon organization is present in other ion channel-blocking toxins from scorpions implying that molecules having similar functions share a similar organization at the genomic level suggesting a common path of evolution.

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“…The general structure of the stem loop is shown in figure 1A, drawn as a six base stem and a four base loop. This structure is found in histone RNAs from all vertebrates, as well as echinoderms (19), cnidaria (20), nematodes (21), annelids (22), Drosophila (19) and Volvox (23).…”

Section: Specific Binding Activities In the Nucleus And Polysomesmentioning

confidence: 96%

Different complexes are formed on the 3′ end of histone mRNA with Nuclear and polyribosomal proteins

1991

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Specific protein-RNA complexes are formed by incubating a synthetic histone mRNA 3' end (a 30 nucleotide stem-loop structure) RNA with extracts of either nuclei or polyribosomes. The complex formed between the stem-loop and nuclear proteins has a lower electrophoretic mobility than the complex formed between the stem-loop and polyribosomal proteins. Binding of the synthetic 3' end by both polyribosomal and nuclear proteins is abolished when two of the conserved uridine residues in the loop are replaced with adenosines. UV crosslinking of the protein complexes to the synthetic RNA resulted in transferring radiolabel to similar sized proteins, 50 kD, in both the nuclear and polyribosomal extracts.

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Nucleotide sequence of the histone H3-encoding gene from the scleractinian coral Acropora formosa (cnidaria: scleractinia)

Cited by 10 publications

References 7 publications

The acute transcriptional response of the coral Acropora millepora to immune challenge: expression of GiMAP/IAN genes links the innate immune responses of corals with those of mammals and plants

The acute transcriptional response of the coral Acropora millepora to immune challenge: expression of GiMAP/IAN genes links the innate immune responses of corals with those of mammals and plants

Genomic structure of a potassium channel toxin from Heteractis magnifica¹

Different complexes are formed on the 3′ end of histone mRNA with Nuclear and polyribosomal proteins

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Nucleotide sequence of the histone H3-encoding gene from the scleractinian coral Acropora formosa (cnidaria: scleractinia)

Cited by 10 publications

References 7 publications

The acute transcriptional response of the coral Acropora millepora to immune challenge: expression of GiMAP/IAN genes links the innate immune responses of corals with those of mammals and plants

The acute transcriptional response of the coral Acropora millepora to immune challenge: expression of GiMAP/IAN genes links the innate immune responses of corals with those of mammals and plants

Genomic structure of a potassium channel toxin from Heteractis magnifica1

Different complexes are formed on the 3′ end of histone mRNA with Nuclear and polyribosomal proteins

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Genomic structure of a potassium channel toxin from Heteractis magnifica¹