Nucleotide sequence of the genes encoding the canine herpesvirus gB, gC and gD homologues

Abstract

The nucleotide sequence of the genes encoding the canine herpesvirus (CHV) gB, gC and gD homologues was determined. These genes are predicted to encode polypeptides of 879, 459 and 345 amino acids, respectively. Comparison of the predicted amino acid sequences of CHV gB, gC and gD with the homologous sequences from other herpesviruses indicates that CHV is an alphaherpesvirus, a conclusion that is consistent with the previous classification of this virus according to biological properties. Alignment of the homologous gB, gC and gD amino acid sequences indicates that most of the cysteine residues are conserved, suggesting that these glycoproteins possess similar tertiary structures. The nucleotide sequence of the open reading frame downstream from the CHV gC gene was also determined. The predicted amino acid sequence of this putative polypeptide appears to be homologous to a family of proteins encoded downstream from the gC gene in most, although not all, alphaherpesviruses.

Gene organization in the UL region and inverted repeats of the canine herpesvirus genome

“…This allowed us to gather about 25 kb of nucleotide sequences, representing an estimated 20 % of the CHV genome. The average G+C content of the sequences was 31"8%, in agreement with previously published data (Goodheart & Plummer, 1975;Limbach et al, 1994). The limited data (approximately 1.5 kb) from the inverted repeats gave a higher value, 38 %, as observed for the inverted repeats of several other herpesviruses (Davison & Scott, 1985;McGeoch et al, 1986;Telford et al, 1992).…”

“…While this work was in progress, Limbach et al (1994) published sequence data on CHV glycoproteins B, C and D, and their analysis of the relationship of CHV to other herpesviruses concurs with our own.…”

“…The CHV thymidine kinase (R6mond et al, 1995) and gC both exhibited greater similarity to the respective proteins of FHV than to those of EHV-1, while the ORF15 homologue showed equivalent similarity with those of both viruses (Table 3; Limbach et al, 1994). Various diverged positional homologues to ORF15 are present in other alphaherpesviruses, but in VZV this ORF has been replaced by a gene for thymidylate synthase (Davison & Scott, 1986).…”

Major immunogenic proteins of phocid herpesviruses and their relationships to proteins of canine and feline herpesviruses

“…The overall pattern and the molecular weights of the other glycoproteins precipitated from PhHV-1-infected cell lysates corresponded closely to those described for CHV and FHV, except for a 154-kDa glycoprotein which appears to be unique to PhHV-1 (5,6,8,21,22,23,30,31,39). A putative glycoprotein D homologue of PhHV-1, by analogy to CHV and FHV (20,22), would be expected to migrate as a single glycoprotein of 55 -65 kDa, but no such glycoprotein was identified in this study.…”

Canine respiratory viruses

“…The gene structure of CHV has yet to be determined, since no CHV strain has been completely sequenced and only a few genes have been identified [73,96,132]. Restriction mapping, southern blot hybridization and sequence analysis have shown that the overall structure of CHV resembles those of other alphaherpesviruses and that CHV is genetically related to feline herpesvirus (FHV-1), phocid herpesvirus 1 and to the equid herpesviruses 1 and 4 [103,130,138,169].…”