Nucleotide Sequence of RNA from the Sobemovirus Found in Infected Cocksfoot Shows a Luteovirus-Like Arrangement of the Putative Replicase and Protease Genes

Ryabov, Eugene V.; Krutov, A. A.; Novikov, V. K.; Zheleznikova, O. V.; Morozov, Sergey Y.; Завриев, С. К.

doi:10.1094/phyto-86-391

Cited by 28 publications

(18 citation statements)

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“…The sgRNA in the molecular weight range of 0.3 ϫ 10 6 to 0.4 ϫ 10 6 of SCPMV and SBMV has a VPg linked to its 5Ј end, as does the genomic RNA (29,62). Sobemovirus RNAs of less than genomic length have also been reported for RYMV-CI (about 1 kb), CfMV-NO (1.2 kb), and CfMV-RU (about 1 kb) (8,61,86). Like those of SBMV and SCPMV, the sgRNA molecules of CfMV are encapsidated (86,95).…”

Section: Gene Products and Their Functionsmentioning

confidence: 98%

“…The complete nucleotide sequences of several sobemoviruses have been determined (58,61,114,70,86,112; A. C. Jeffries, J. P. Rathjen, and R. H. Symons, GenBank accession no. U31286; Y. M. Pinto and D. C. Baulcombe, GenBank accession no.…”

Section: Genome Organizationmentioning

confidence: 99%

“…In contrast, the Norwegian (CfMV-NO) and Russian (CfMV-RU) isolates of CfMV lack the continuous ORF2 and a nested coding region similar to ORF3 of SCPMV (61,86). Instead, CfMV has two overlapping ORFs, ORF2a and ORF2b, and the polyprotein is expressed through a Ϫ1 ribosomal frameshift mechanism.…”

Section: Genome Organizationmentioning

confidence: 99%

“…These results demonstrate that the genomic RNAs and sgRNAs of sobemoviruses start with very similar primary sequences and that these are potentially important for virus replication. In contrast, this sequence motif is not present at the 5Ј end of the CfMV genome (61,86). All sobemoviruses have a polypurine tract, including the sequence aAGgAAA (lowercase indicates less conservation of that base) just at the beginning of the genomic RNA (36).…”

Section: Gene Products and Their Functionsmentioning

confidence: 99%

“…Even less is known about the replication signals needed to initiate minus-strand synthesis at the 3Ј ends of sobemovirus genomic RNAs. A potential tRNA-like structure has been attributed to the 3Ј end of some sobemovirus genomic RNAs (86,114). However, for SBMV and SCPMV, it has been impossible to find an RNA sequence at the 3Ј end that has the potential to fold into a tRNA-resembling secondary structure (70,112).…”

Section: Gene Products and Their Functionsmentioning

confidence: 99%

See 4 more Smart Citations

Sobemoviruses

Tamm

Truve

2000

J Virol

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Section: Gene Products and Their Functionsmentioning

confidence: 98%

Section: Genome Organizationmentioning

confidence: 99%

Section: Genome Organizationmentioning

confidence: 99%

Section: Gene Products and Their Functionsmentioning

confidence: 99%

Section: Gene Products and Their Functionsmentioning

confidence: 99%

See 3 more Smart Citations

Sobemoviruses

Tamm

Truve

2000

J Virol

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The 105-kDa Polyprotein of Southern Bean Mosaic Virus Is Translated by Scanning Ribosomes

Sivakumaran

Hacker

1998

Virology

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The cowpea strain of southern bean mosaic virus (SBMV-C) is a positive-sense RNA virus. Three open reading frames (ORF-1, ORF2, and ORF3) are expressed from the genomic RNA. The ORF1 and ORF2 initiation codons are located at nucleotide (nt) positions 49 and 570, respectively. ORF1 is expressed by a 5' end-dependent scanning mechanism, but it is not known how ribosomes gain access to the ORF2 initiation codon. In experiments described here, it was demonstrated that the translation of ORF2 was sensitive to cap analog in a cell-free extract. In vitro and in vivo studies showed that the addition of one or more AUG codons between the 5' end of the SBMV-C RNA and the ORF2 initiation codon reduced ORF2 expression and that elimination of the ORF1 initiation codon increased ORF2 expression. Altering the sequence context of the ORF1 initiation codon to one more favorable for translation initiation also reduced ORF2 expression in vivo. Nucleotide deletions and insertions between SBMV-C nt 218-520 did not abolish ORF2 expression. In most cases, these mutations resulted in reduced expression of both ORF1 and ORF2. These results are consistent with translation of ORF2 by leaky scanning.

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Identification of Viral Genes Required for Cell-to-Cell Movement of Southern Bean Mosaic Virus

1998

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Inoculation of Vigna unguiculata (cowpea) with transcripts synthesized in vitro from a genome-length cDNA clone of the cowpea strain of southern bean mosaic virus (SBMV-C) resulted in a systemic SBMV-C infection of this host. Capped RNA was about five times more infectious than uncapped RNA as determined by a local lesion assay. The SBMV-C cDNA clone was also used for mutagenesis of the four SBMV-C open reading frames (ORFs). ORF1, ORF3, and coat protein (CP) mutants were not infectious in cowpea. Electroporation of cowpea protoplasts with mutant transcripts demonstrated that the ORF1, ORF3, and CP gene products were not required for SBMV-C RNA synthesis, and the ORF1 and ORF3 gene products were not required for SBMV-C assembly. From these results, it was concluded that the ORF1 and ORF3 proteins and the CP are required for SBMV-C cell-to-cell movement. One of the ORF3 mutants pSBMV2-UAA1833 contained a nonsense codon between the predicted -1 ribosomal frameshift site (SBMV-C nucleotides 1796-1802) and a potential ORF3 translation initiation codon at SBMV-C nucleotide 1895. The lack of infectivity of this mutant suggested that ORF3 was expressed by a -1 ribosomal frameshift in ORF2 rather than by initiation of translation at nucleotide 1895.

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Nucleotide Sequence of RNA from the Sobemovirus Found in Infected Cocksfoot Shows a Luteovirus-Like Arrangement of the Putative Replicase and Protease Genes

Cited by 28 publications

References 0 publications

Sobemoviruses

Sobemoviruses

The 105-kDa Polyprotein of Southern Bean Mosaic Virus Is Translated by Scanning Ribosomes

Identification of Viral Genes Required for Cell-to-Cell Movement of Southern Bean Mosaic Virus

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