Nucleotide sequence of RNA 3 of peanut stunt cucumovirus

Karasawa, Akira; Nakaho, Kazuhiro; Kakutani, Tetsuji; Minobe, Yuzo; Ehara, Yoshio

doi:10.1016/0042-6822(91)90800-q

Cited by 34 publications

(26 citation statements)

References 20 publications

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“…The UG tract of PSV-W occurs between position 85 and 100 ; those for strains ER and J occur between positions 88 and 103, and 87 and 102, respectively (Figs 3 and 4 ; Karasawa et al, 1991). Interestingly, TAV has a significantly longer UG tract (between position 38 and 80 ; O'Reilley et al, 1991) than either PSV or CMV.…”

Section: Comparative Nucleotide Sequence Analysis Of Rna3 From Psv Stmentioning

confidence: 94%

“…The values for percentage nucleotide sequence identity between strains in the same subgroup (between strains J and ER) are 91 and 94 %, respectively, for RNA1 and RNA2. The corresponding values for strains in different subgroups (between strains W and ER\J) are 79 and 76 %, respectively, for RNA1 and RNA2 (C.-C. Hu and others, unpublished ; Karasawa et al, 1992). …”

Section: Comparative Nucleotide Sequence Analysis Of Rna3 From Psv Stmentioning

confidence: 95%

“…In both strains, the initiation codon of ORF1 (which is the first AUG in the sequence) has an optimal context, according to Kozak (1989) : an A at position k3 and a G at position j4. Unlike strains W and ER, PSV-J RNA3 has two AUG codons in frame proximal to the 5h terminus (Karasawa et al, 1991), at positions 113-115 and 119-121 (underlined and double-underlined, respectively, in alignment below). Karasawa et al (1991) were not certain as to which one of the AUGs is the initiator codon.…”

Section: Characterization Of the Psv 3a Protein And Comparison With Omentioning

confidence: 99%

“…Partial sequences of some TAV strains are also available (Moriones et al, 1991 ;O'Reilly et al, 1991). Only the complete sequence of the Japanese strain of PSV (Karasawa et al, 1991(Karasawa et al, , 1992 has been published. In this paper we report the complete nucleotide sequence of RNA3 from two strains of PSV and present serological and molecular evidence that strains of PSV, like those of CMV, fall into two distinct subgroups.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA3.

Aboul-Ata

Naidu

et al. 1997

Journal of General Virology

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Strains of peanut stunt cucumovirus (PSV) were classified into two distinct subgroups, I and II, based on Western and Northern blot analyses using antisera and cloned cDNA probes to strains PSV-ER and PSV-W. These results were corroborated by nucleotide sequence analyses of full-length cDNA clones of RNA3 from representative strains of the two subgroups. Whereas the percentage nucleotide sequence identity between PSV-ER (or PSV-J) and PSV-W RNA3s was determined to be 80 %, the corresponding value between strains ER and J was 91 %, confirming that strains ER and J belong to the same subgroup (subgroup I) whereas strain W belongs to a separate subgroup (subgroup II). PSV-W and PSV-ER RNA3s are 2173 and 2188 nucleotides long, respectively. Each is dicistronic,

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Section: Comparative Nucleotide Sequence Analysis Of Rna3 From Psv Stmentioning

confidence: 94%

Section: Comparative Nucleotide Sequence Analysis Of Rna3 From Psv Stmentioning

confidence: 95%

Section: Characterization Of the Psv 3a Protein And Comparison With Omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA3.

Aboul-Ata

Naidu

et al. 1997

Journal of General Virology

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show abstract

“…Fny-CMV, the V and C strains of tomato aspermy virus (V-TAV, C-TAV) and the J strain of peanut stunt virus (J-PSV) have been described in previous studies (Karasawa et al, 1991 ;O'Reilly et al, 1991 ;Perry & Francki, 1992 ;Perry et al, 1998). Virus was purified from infected Nicotiana tabacum or N. clevelandii by the method of Lot et al (1972).…”

Section: Methodsmentioning

confidence: 99%