Nucleotide sequence of Pseudomonas aeruginosa conjugative plasmid pUM505 containing virulence and heavy-metal resistance genes

Ramírez-Díaz, Martha I.; Díaz-Magaña, Amada; Meza-Carmen, Víctor; Johnstone, Laurel; Cervantes, Carlos; Rensing, Christopher

doi:10.1016/j.plasmid.2011.03.002

Cited by 37 publications

(30 citation statements)

References 41 publications

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“…In another case, Vibrio cholerae and Vibrio pahraemoliticus isolated from water and shrimps were resistant to Cu, Cd, Hg, Zn, and Pb (Song et al, 2013). Staphylococcus aureus strains from humans were found resistant to Cu and Cd (Gómez-Sanz et al, 2013), and a clinical Pseudomonas aeruginosa isolate was resistant to Cu and Zn (Ramírez-Díaz et al, 2011).…”

Section: Heavy Metal Pollution and Resistancementioning

confidence: 97%

Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

2016

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Section: Heavy Metal Pollution and Resistancementioning

confidence: 97%

Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

2016

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“…Here we present the largest intraspecies study of type II TA systems, to our knowledge, of the opportunistic pathogen Pseudomonas aeruginosa . Some TA systems have previously been identified from P. aeruginosa computationally, and found chromosomally and on mobile elements, either integrated as prophages and ICEs or on plasmids (Webb et al, 2004; Pandey and Gerdes, 2005; Ramirez-Diaz et al, 2011; Ou et al, 2012; Bonnin et al, 2013; Li et al, 2016a). Two systems, HicBA and HigAB, were recently confirmed experimentally (Li et al, 2016b; Wood and Wood, 2016).…”

Section: Introductionmentioning

confidence: 99%

Diversity, Prevalence, and Longitudinal Occurrence of Type II Toxin-Antitoxin Systems of Pseudomonas aeruginosa Infecting Cystic Fibrosis Lungs

et al. 2017

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Type II toxin-antitoxin (TA) systems are most commonly composed of two genes encoding a stable toxin, which harms the cell, and an unstable antitoxin that can inactivate it. TA systems were initially characterized as selfish elements, but have recently gained attention for regulating general stress responses responsible for pathogen virulence, formation of drug-tolerant persister cells and biofilms—all implicated in causing recalcitrant chronic infections. We use a bioinformatics approach to explore the distribution and evolution of type II TA loci of the opportunistic pathogen, Pseudomonas aeruginosa, across longitudinally sampled isolates from cystic fibrosis lungs. We identify their location in the genome, mutations, and gain/loss during infection to elucidate their function(s) in stabilizing selfish elements and pathogenesis. We found (1) 26 distinct TA systems, where all isolates harbor four in their core genome and a variable number of the remaining 22 on genomic islands; (2) limited mutations in core genome TA loci, suggesting they are not under negative selection; (3) no evidence for horizontal transmission of elements with TA systems between clone types within patients, despite their ability to mobilize; (4) no gain and limited loss of TA-bearing genomic islands, and of those elements partially lost, the remnant regions carry the TA systems supporting their role in genomic stabilization; (5) no significant correlation between frequency of TA systems and strain ability to establish as chronic infection, but those with a particular TA, are more successful in establishing a chronic infection.

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“…DNA for Southern blot assays was isolated by the alkaline lysis procedure described above, separated by agarose gel electrophoresis, and transferred to nitrocellulose membranes by capillarity. The coding region of the chrA gene was utilized as a probe for chromate-resistance (Cr R ) genes; a 1.25-kb fragment was PCR-amplified from the pEPL1 plasmid (7.7 kb), which contains a BamHI-PstI 3.8-kb fragment bearing the pUM505 chrA gene cloned in the pUCP20 vector (Ramírez-Díaz et al, 2011). PCR was conducted employing forward oligonucleotide 1D (5′-GAGCGTTG CGAATGAAGAGTCG-3′) and reverse oligonucleotide 1R (5′-GGAAGCATGAAACCGAGTCCC-3′).…”

Section: Hybridization Assaysmentioning

confidence: 99%

Chromate-resistance genes in plasmids from antibiotic-resistant nosocomial enterobacterial isolates

Caballero-Flores¹,

Acosta-Navarrete²,

Ramírez-Díaz³

et al. 2011

FEMS Microbiol Lett

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The presence of chromate-resistance genes in enterobacteria was evaluated in a collection of 109 antibiotic-resistant nosocomial isolates from nine major cities in México. Results were compared with the presence of mercury-resistance genes. Susceptibility tests showed that 21% of the isolates were resistant to chromate (Cr(R)), whereas 36% were resistant to mercury (Hg(R)). Cr(R) levels were high in Klebsiella pneumoniae (61%), low in Enterobacter cloacae (12%) and Escherichia coli (4%), and null in Salmonella sp. isolates. Colony hybridization demonstrated that the majority of metal-resistant isolates hybridized with chrA gene (87% of Cr(R) isolates), encoding a CHR transporter homologue, and merA gene (74% of Hg(R) isolates), encoding MerA mercuric reductase, suggesting that most isolates expressed these widespread metal-resistance systems. Southern blot hybridization of Cr(R) isolates showed that plasmids of 80, 85, and 95 kb from K. pneumoniae isolates, and of 100 kb from an E. cloacae isolate, contained chrA-related sequences. These plasmids belonged to IncN or IncP incompatibility groups, and conferred Cr(R), as well as multiple antibiotic resistance, when transferred by conjugation to an E. coli standard strain. These data indicated that Cr(R) genes may be distributed among clinical enterobacteria via conjugative plasmids, probably by coselection with antibiotic-resistant genes.

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Nucleotide sequence of Pseudomonas aeruginosa conjugative plasmid pUM505 containing virulence and heavy-metal resistance genes

Cited by 37 publications

References 41 publications

Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

Diversity, Prevalence, and Longitudinal Occurrence of Type II Toxin-Antitoxin Systems of Pseudomonas aeruginosa Infecting Cystic Fibrosis Lungs

Chromate-resistance genes in plasmids from antibiotic-resistant nosocomial enterobacterial isolates

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