Nucleotide sequence of Moloney murine leukaemia virus

Shinnick, Thomas M.; Lerner, Richard A.; Sutcliffe, J. Gregor

doi:10.1038/293543a0

Cited by 1,074 publications

(768 citation statements)

References 95 publications

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“…(iii) The notion that a stream of 80S ribosomes advancing from an upstream initiation site might interfere to some extent with initiation at downstream sites could not be dismissed a priori at the outset of this study, although it seemed unlikely that such a mechanism would completely suppress downstream initiation--as was observed with p255/20. [In procaryotic systems, the advance of 70S ribosomes from an upstream initiation site often reduces, but never abolishes, the translation of an overlapping gene (22)(23)(24) [27][28][29]. In a few other cases, the 5'-and 3'-proximal cistrons do not overlap; but the presence of several AUG triplets within the intercistronic region might attenuate the movement of 40S ribosomal subunits through that region (30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin

1984

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Recombinant plasmids that direct synthesis of rat preproinsulin under the direction of the SV40 early promoter have been used to probe the mechanism of initiation of translation. Insertion of an upstream AUG triplet that was outof-frame with respect to the coding sequence for preproinsulin red iced the yield of proinsulin, in keeping with the predictions of the scanning model. The extent to which an upstream AUG codon interfered depended on sequences surrounding the AUG triplet; with two constructs (p255/20 and C2) the 5'-proximal AUG codon constituted an absolute barrier: there was no initiation at the downstream start site for preproinsulin. With two other constructs (p255/9, p255/21), however, proinsulin was made despite the presence of an upstream, out-of-frame AUG codon in a favorable context for initiation. In those cases the reading frame set by the first AUG triplet was short, terminating before the start of the preproinsulin coding sequence. The interpretation that ribosomes initiate at the first AUG, terminate, and then reinitiate at the AUG that directly precedes the preproinsulin coding sequence was tested by introducing a point mutation that eliminated the terminator codon: the resulting mutant made no proinsulin.

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Section: Discussionmentioning

confidence: 99%

Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin

1984

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“…The gag and pol genes are in the same reading frame and expression of the latter occurs via suppression of the gag gene termination codon. The region spanning the end of the gag gene and the beginning of thepol gene (encompassing the amber termination codon), encodes the viral protease responsible for cleavage of the gag polyprotein precursor into the mature core proteins [45]. The protease from MO-MuLV [32] has been sequenced establishing the suppression event and revealing the presence of a Gln inserted in response to the nonsense codon (see section 3.2).…”

Section: Retroviral Systemsmentioning

confidence: 99%

Stop making sense or Regulation at the level of termination in eukaryotic protein synthesis

Valle

Morch

1988

FEBS Letters

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“…Such species hybridize also with env and pX probes but not with the pol probe (panels 1-3, lanes b-e) and must derive from a post-transcriptional processing of the HTLV genomic RNA. We believe that the sequences hybridizing in probe b are located prior to the donor splice site that is located at the 5' region of all of the animal retroviruses studied (33)(34)(35)(36)(37). Since these mRNA species are the only ones that contain env sequences in MJ and UK RNA, they must encode for the envelope protein.…”

Section: Detection Of Htlv-i Provirus In the Dnas Of The Fresh Leukemmentioning

confidence: 99%

Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia.

Franchini¹,

Wong‐Staal²,

Gallo³

1984

Proc. Natl. Acad. Sci. U.S.A.

143

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We examined the DNA and RNA of fresh leukemic cells (five patients) and long-term cultured T lymphocytes (four patients) from patients with adult T-cell leukemia for the presence of human T-cell leukemia virus (HTLV-I) sequences. In all cases HTLV-I previrus was found and several species of viral mRNAs were observed in all cell lines. We used HTLV DNA probes representing the gag, pol, and env genes and pX regions and the U3-and US-specific sequences to characterize the genetic content of the viral transcripts. Although similar RNA sequences were expressed in Om HTLV-I provirus-positive specimen of fresh primary leukemic cells, no viral transcripts were found in four other similar specimens. These findings are consistent with the idea that the expression of one or more HTLV-I genes may be involved in initiation of transformation, but consistent expression is not needed for maintaining the neoplastic state.

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Nucleotide sequence of Moloney murine leukaemia virus

Cited by 1,074 publications

References 95 publications

Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin

Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin

Stop making sense or Regulation at the level of termination in eukaryotic protein synthesis

Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia.

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