Nucleotide sequence of<i>Candida pelliculosa</i>β-glucosidase gene

Kohchi, Chie; Toh‐e, Akio

doi:10.1093/nar/13.17.6273

Cited by 52 publications

(21 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In all the sequences examined by Barnett et al (1991) the two conserved motifs were separated by 12 amino acid residues. It is now clear that there are some glucosidases, including those encoded by the celD gene for (1→4)-β-D-glucan glucohydrolase from P. fluorescens (Rixon et al, 1992), a β-glucosidasehomologous (LacZ) gene from Listeria monocytogenes (He et al (1996), accession no: U78883), a β-glucosidase gene from Candida pelliculosa (Kohchi and Toh-e, 1985) and the β-glucosidase bgln gene from Pichia capsulata (Janbon et al, 1995), in which the two motifs are separated by more than 20 residues. However, the nasturtium enzyme and the barley exohydrolase are uniquely different from all other sequences so far reported, showing the motifs S-D-W and G-I-D-M, separated by a sequence of 24 amino acids which is almost perfectly conserved (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

A xyloglucan oligosaccharide‐active, transglycosylating‐D‐glucosidase from the cotyledons of nasturtium (Tropaeolum majus L) seedlings – purification, properties and characterization of a cDNA clone

et al. 1998

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

A xyloglucan oligosaccharide‐active, transglycosylating‐D‐glucosidase from the cotyledons of nasturtium (Tropaeolum majus L) seedlings – purification, properties and characterization of a cDNA clone

et al. 1998

View full text Add to dashboard Cite

“…It has been proposed that ,-glucosidase in some bacteria and yeasts is a periplasmic protein (1,12,28). One characteristic structure associated with many periplasmic proteins and other secreted proteins is a leader sequence, which targets a protein for transport across membranes (24).…”

Section: And Discussionmentioning

confidence: 99%

“…At present, only the following two complete ,-glucosidase gene sequences are known: abg (this work) and the Candida pelliculosa gene, cpb (12). Part of the (21).…”

Section: And Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400

et al. 1988

View full text Add to dashboard Cite

The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3' termini. A putative abg promoter was identified whose sequence shows similarities to the consensus promoter of Escherichia coli and with the nif promoter regions of Klebsiella. The abg coding sequence was 1,374 nucleotides long. The molecular weight of the enzyme, based on the predicted amino acid sequence, was 51,000. The observed Mr was 50,000 to 52,000. A region of deduced protein sequence was homologous to a region from two other beta-glucosidase sequences. This region of homology contained a putative active site by analogy with the active site of hen egg white lysozyme.

show abstract

“…Both enzymes are aryl-,-glucosidases, but only Bgll is able to hydrolyze cellobiose. Clhq from Candida pelliculosa (17) and Cbgl are 29% identical and 54% similar in sequence. Clhq also has a signal peptide for transport into the periplasmic space.…”

Section: Gcttgccaca Gaccttcccg Aaggcgctca Cggataattc Cgccattacc Gacgamentioning

confidence: 99%

Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule

1992

View full text Add to dashboard Cite

Induction of Agrobacterium tumefaciens virulence genes by plant phenolic compounds is essential for successful T-DNA transfer to a host plant. In Douglas fir needles, the major virulence region inducer is the glycoside coniferin (J. W. Morris and R. 0. Morris, Proc. NatI. Acad. Sci. USA 87:3612-3618, 1990).Agrobacterium strains with high fl-glucosidase activity respond to coniferin and infect Douglas fir seedlings, whereas most strains with low 13-glucosidase activity fail to respond to coniferin and are avirulent on this host.We have cloned two ,8-glucosidase genes from A. tumefaciens B3/73 and sequenced one of them, cbgl. It appears to be part of a polycistronic unit and shows a high bias for GC-rich codons. When expressed in Escherichia coli, Cbgl j-glucosidase hydrolyzes coniferin but not cellobiose. The 88-kDa predicted product of cbgl is highly similar to one other bacterial 13-glucosidase and several fungal l-glucosidases. There is little homology between Cbgl and other bacterial 13-glucosidases, including an Agrobacterium cellobiase.Communication between Agrobactenum tumefaciens and host plant cells is essential for successful tumorigenesis. During infection, Ti plasmid genes of the virulence (vir) region control transfer of the T-DNA from the bacterial cell to the plant cell. Genes of the virulence region, first defined by transposon mutagenesis (8), were found to be transcriptionally activated by plant exudates and suspension-cultured cells (15,43). Two of the virulence products, VirA and VirG, are essential for induction of the other loci (42). These two proteins share conserved domains found in many bacterial two-component environmental responsive systems (29,51); VirA is similar to other sensor proteins, and VirG shows homology to other transcriptional activators.The first native vir region-inducing compounds were isolated from tobacco root exudates (41). Acetosyringone and hydroxy-acetosyringone were identified. Spencer and Towers (40) and Melchers et al. (25) systematically tested a large number of synthetic phenolic compounds to determine the structural requirements of active inducers. They found that most active compounds were composed of substituted benzene rings with free 4'-hydroxyl groups, 3'-methoxy and often 5'-methoxy groups, and usually an electron-withdrawing group at the 1' position. Flavonoid compounds purified from petunia pollen and stigma tissues have also been identified as vir region inducers (54). Coniferin, purified from Douglas fir (Pseudotsuga menziesii) needle extracts, was identified as the major native vir region inducer in that species (28). It differs from other inducers in that it is a glucoside in which the 4'-hydroxyl group is blocked. Hess et al. (12)

show abstract

Nucleotide sequence ofCandida pelliculosaβ-glucosidase gene

Cited by 52 publications

References 14 publications

A xyloglucan oligosaccharide‐active, transglycosylating‐D‐glucosidase from the cotyledons of nasturtium (Tropaeolum majus L) seedlings – purification, properties and characterization of a cDNA clone

A xyloglucan oligosaccharide‐active, transglycosylating‐D‐glucosidase from the cotyledons of nasturtium (Tropaeolum majus L) seedlings – purification, properties and characterization of a cDNA clone

Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400

Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule

Contact Info

Product

Resources

About