Nucleotide sequence of HIV1-NDK: a highly cytopathic strain of the human immunodeficiency virus

Spire, Bruno; Sire, Joséphine; Zachar, Vladimír; Rey, Françoise; Barré‐Sinoussi, Françoise; Galibert, Francis; Hampe, A; Chermann, Jean‐Claude

doi:10.1016/0378-1119(89)90188-1

Cited by 121 publications

(63 citation statements)

References 34 publications

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“…U373MG-CD4 are stably transfected with a HIV-inducible ␤-galactosidase reporter gene (LTR-lacZ). HIV-1 stocks were derived from supernatants of HeLa cells transfected with a cloned LAI provirus (42), or of CEM cells chronically infected with the NDK strain (43). Titers were ϳ10 5 infectious units (I.U.)…”

Section: Methodsmentioning

confidence: 99%

Identification of Residues of CXCR4 Critical for Human Immunodeficiency Virus Coreceptor and Chemokine Receptor Activities

Brelot¹,

Heveker²,

Montes³

et al. 2000

Journal of Biological Chemistry

232

287

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CXCR4 is a G-coupled receptor for the stromal cellderived factor (SDF-1) chemokine, and a CD4-associated human immunodeficiency virus type 1 (HIV-1) coreceptor. These functions were studied in a panel of CXCR4 mutants bearing deletions in the NH 2 -terminal extracellular domain (NT) or substitutions in the NT, the extracellular loops (ECL), or the transmembrane domains (TMs). The coreceptor activity of CXCR4 was markedly impaired by mutations of two Tyr residues in NT (Y7A/ Y12A) or at a single Asp residue in ECL2 (D193A), ECL3 (D262A), or TMII (D97N). These acidic residues could engage electrostatical interactions with basic residues of the HIV-1 envelope protein gp120, known to contribute to the selectivity for CXCR4. The ability of CXCR4 mutants to bind SDF-1 and mediate cell signal was consistent with the two-site model of chemokine-receptor interaction. Site I involved in SDF-1 binding but not signaling was located in NT with particular importance of Glu 14 and/or Glu 15 and Tyr 21 . Residues required for both SDF-1 binding and signaling, and thus probably part of site II, were identified in ECL2 (Asp 187 ), TMII (Asp 97 ), and TMVII (Glu 288 ). The first residues (2-9) of NT also seem required for SDF-1 binding and signaling. A deletion in the third intracellular loop abolished signaling, probably by disrupting the coupling with G proteins. The identification of CXCR4 residues involved in the interaction with both SDF-1 and HIV-1 may account for the signaling activity of gp120 and has implications for the development of antiviral compounds.

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Section: Methodsmentioning

confidence: 99%

Identification of Residues of CXCR4 Critical for Human Immunodeficiency Virus Coreceptor and Chemokine Receptor Activities

Brelot¹,

Heveker²,

Montes³

et al. 2000

Journal of Biological Chemistry

232

287

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“…Amino Viruses. Laboratory strains HIV-1(LAI) (2), HIV-1(NDK) (34), and HIV-2(ROD) (8) were produced in CEM cells. Macrophage-tropic HIV-1(89.6) (9) was produced in CEMx174 cells as previously described (35).…”

Section: Methodsmentioning

confidence: 99%

Multibranched V3 peptides inhibit human immunodeficiency virus infection in human lymphocytes and macrophages

Yahi¹,

Fantini²,

Mabrouk³

et al. 1995

Peptides 1994

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“…The HIV-I strains used in our studies were: strain HTLVIIIs (LAI) and strain RF, originating from a patient of Haitian descent (Starcich et al, 1986), both obtained from R. C. Gallo, National Institutes of Health, Bethesda, Md., U.S.A. ; strain HE, isolated by us from a Belgian patient with AIDS, and strain NDK, a highly cytopathic strain (Spire et al, 1989), obtained from J. C. Chermann (INSERM, Marseilles, France). The HIV-2 strain ROD (Clavel et al, 1986) was provided by L. Montagnier, Pasteur Institute, Paris, France and SIVma~251 (Franchini et al, 1987) was received from C. Bruck, SmithKline Beecham, Genval, Belgium.…”

Section: Methodsmentioning

confidence: 99%

Differential inhibitory effects of TIBO derivatives on different strains of simian immunodeficiency virus

Debyser

Vreese

Pauwels

et al. 1992

Journal of General Virology

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Nucleotide sequence of HIV1-NDK: a highly cytopathic strain of the human immunodeficiency virus

Cited by 121 publications

References 34 publications

Identification of Residues of CXCR4 Critical for Human Immunodeficiency Virus Coreceptor and Chemokine Receptor Activities

Identification of Residues of CXCR4 Critical for Human Immunodeficiency Virus Coreceptor and Chemokine Receptor Activities

Multibranched V3 peptides inhibit human immunodeficiency virus infection in human lymphocytes and macrophages

Differential inhibitory effects of TIBO derivatives on different strains of simian immunodeficiency virus

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