Nucleotide sequence of coliphage HK620 and the evolution of lambdoid phages

Clark, Alvin J.; Inwood, William; Cloutier, Thomas; Dhillon, T. S.

doi:10.1006/jmbi.2001.4868

Cited by 110 publications

(105 citation statements)

References 47 publications

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“…However, prophage induction by an antirepressor usually implies that the repressor should lack the self-catalytic residues, which is not the case for HkaT (NP_112053.1). We thus analyzed the transcripts that arose from the P R promoter, which is the main lytic promoter in P22-like phages (27,41). As shown in Fig.…”

Section: Bcm Inhibition Of Rho Induces Prophage Hk620 In An Sos-indepmentioning

confidence: 99%

“…We thus hypothesized that if prophage maintenance was controlled at the gene regulation level, the target should be the RDF gene. We tested and validated our prediction by using two different prophage models, KplE1 and HK620, which both integrate at the integration hot-spot argW in E. coli MG1655 and TD2158, respectively (25)(26)(27). KplE1 (also named CPS-53) is highly defective (it has only a 10-kb genome).…”

mentioning

confidence: 99%

“…However, it is fully competent for site-specific recombination and provides fitness to its host under oxidative stress conditions (28)(29)(30). HK620 is a fully infectious P22-like phage that shares a highly conserved site-specific recombination module with KplE1 (27,28).…”

mentioning

confidence: 99%

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Transcription termination controls prophage maintenance in Escherichia coli genomes

Menouni

Champ

Espinosa

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Prophages represent a large fraction of prokaryotic genomes and often provide new functions to their hosts, in particular virulence and fitness. How prokaryotic cells maintain such gene providers is central for understanding bacterial genome evolution by horizontal transfer. Prophage excision occurs through site-specific recombination mediated by a prophage-encoded integrase. In addition, a recombination directionality factor (or excisionase) directs the reaction toward excision and prevents the phage genome from being reintegrated. In this work, we describe the role of the transcription termination factor Rho in prophage maintenance through control of the synthesis of transcripts that mediate recombination directionality factor expression and, thus, excisive recombination. We show that Rho inhibition by bicyclomycin allows for the expression of prophage genes that lead to excisive recombination. Thus, besides its role in the silencing of horizontally acquired genes, Rho also maintains lysogeny of defective and functional prophages.bacteriophage | transduction | transcriptional regulation

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Section: Bcm Inhibition Of Rho Induces Prophage Hk620 In An Sos-indepmentioning

confidence: 99%

mentioning

confidence: 99%

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Transcription termination controls prophage maintenance in Escherichia coli genomes

Menouni

Champ

Espinosa

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…For example, the opportunistic symbiont Wolbachia also has high rates of infection by a bacteriophage (WO), and WO from Wolbachia in divergent insect hosts undergo genetic exchange and recombination (27,57,58), thus providing one of the main sources of variation among Wolbachia isolates (59). Similar mosaicism is evident in the genome of APSE-1 (8). Coinfection of host insects by different symbiont isolates, which occurs in both Wolbachia and in aphid secondary symbionts, may enable the exchange of genes through recombination.…”

Section: The Apse-h Defensa-aphid Consortium As a Complex Mutualisticmentioning

confidence: 99%

“…Microscopy has revealed that H. defensa can live within the bacteriocytes that normally house Buchnera, within some other cell types, and extracellularly in the insect hemocoel (1,2). In a European pea aphid strain, secondary symbionts were found to harbor an active bacteriophage, designated APSE-1; its genome sequence indicated a relationship to P22, the lambdoid phage of Salmonella enterica (7,8). The secondary symbiont serving as host to APSE-1 was not identified initially, but, based on a survey of aphid species harboring different symbionts (2), APSE-1-like phage appear to be consistently and exclusively associated with H. defensa.…”

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confidence: 99%

The players in a mutualistic symbiosis: Insects, bacteria, viruses, and virulence genes

Moran

Degnan

Santos

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

289

271

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Aphids maintain mutualistic symbioses involving consortia of coinherited organisms. All possess a primary endosymbiont, Buchnera, which compensates for dietary deficiencies; many also contain secondary symbionts, such as Hamiltonella defensa, which confers defense against natural enemies. Genome sequences of uncultivable secondary symbionts have been refractory to analysis due to the difficulties of isolating adequate DNA samples. By amplifying DNA from hemolymph of infected pea aphids, we obtained a set of genomic sequences of H. defensa and an associated bacteriophage. H. defensa harbors two type III secretion systems, related to those that mediate host cell entry by enteric pathogens. The phage, called APSE-2, is a close relative of the previously sequenced APSE-1 but contains intact homologs of the gene encoding cytolethal distending toxin (cdtB), which interrupts the eukaryotic cell cycle and which is known from a variety of mammalian pathogens. The cdtB homolog is highly expressed, and its genomic position corresponds to that of a homolog of stx (encoding Shiga-toxin) within APSE-1. APSE-2 genomes were consistently abundant in infected pea aphids, and related phages were found in all tested isolates of H. defensa, from numerous insect species. Based on their ubiquity and abundance, these phages appear to be an obligate component of the H. defensa life cycle. We propose that, in these mutualistic symbionts, phage-borne toxin genes provide defense to the aphid host and are a basis for the observed protection against eukaryotic parasites.Acrythosiphon pisum ͉ Hamiltonella defensa ͉ lamboid phage ͉ aphid ͉ Enterobacteriaceae

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Lambdoid Phage

2004

Encyclopedic Dictionary of Genetics, Genomics and Proteomics

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Nucleotide sequence of coliphage HK620 and the evolution of lambdoid phages

Cited by 110 publications

References 47 publications

Transcription termination controls prophage maintenance in Escherichia coli genomes

Transcription termination controls prophage maintenance in Escherichia coli genomes

The players in a mutualistic symbiosis: Insects, bacteria, viruses, and virulence genes

Lambdoid Phage

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