Nucleotide sequence of an arginine transfer ribonucleic acid from bacteriophage T4.

Mazzara, Gail P.; Seidman, J. G.; McClain, William H.; H, Yesian; Abelson, John; Guthrie, Christine

doi:10.1016/s0021-9258(17)40963-x

Cited by 22 publications

(2 citation statements)

References 19 publications

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“…Several lines of A evidence, including hybridization to T4 DNA of aminoacylated tRNAs from T4-infected cells (Scherberg & Weiss, 1970), have suggested that T4 codes for an isoleucine acceptor activity. The nucleotide sequences of the other seven T4 tRNAs have now been determined and include species specific for serine (McClain et al, 1975), proline (Seidman et al, 1975), glutamine (Seidman et al, 1974), leucine (Pinkerton et al, 1973), glycine (Barrell et al, 1973;Stahl et al, 1974), arginine (Mazzara et al, 1977), and threonine (Guthrie et al, 1978). We thus conclude that the molecule we have sequenced corresponds to the acceptor activity identified by Scherberg & Weiss (1970) and infer that it is a tRNA!le.…”

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confidence: 65%

Rare transfer ribonucleic acid essential for phage growth. Nucleotide sequence comparison of normal and mutant T4 isoleucine-accepting transfer ribonucleic acid

Guthrie¹,

McClain²

1979

Biochemistry

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One of the eight tRNA species coded by bacteriophage T4 is unique in that (1) it is found in a yield lower by three- to fourfold than that of any other tRNA and (2) while dispensable for growth in standard laboratory hosts, it is essential for phage propagation in a natural isolate of Escherichia coli (strain CT439). We report here the nucleotide sequence of this tRNA and of several mutationally altered forms. The molecule is 77 nucleotides in length and has the anticodon N-A-U. Depending on the pairing properties of the "wobble" nucleotide N, this sequence could correspond to one or more of the isoleucine-specific codons (formula: see text) or to the methionine-specific codon A-U-G. Since a T4-specific acceptor activity for isoleucine which is stimulated in ribosome binding by A-U-A but not A-U-U has been reported previously, we infer that we have sequenced a tRNA Ile species which preferentially recognizes A-U-A. Mutant HA1 is unable to grow in CT439; it produces no tRNA Ile. The primary mutational alteration is a transition four residues from the 5'terminus which converts a C.G to a U.G base pair. The consequences of this lesion can be partially reversed by second-site mutations nearby in the acceptor stem. Unexpectedly, the tRNA Ile synthesized in these revertants still retains two unusual structural features found in the wild-type molecule: the opposition of two Up residues in the amino acid acceptor stem and the opposition of an Ap and a Gp residue in the anticodon stem. Implications of these structual anomalies for a possibly unique physiological role of this minor tRNA species are discussed.

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confidence: 65%

Rare transfer ribonucleic acid essential for phage growth. Nucleotide sequence comparison of normal and mutant T4 isoleucine-accepting transfer ribonucleic acid

Guthrie¹,

McClain²

1979

Biochemistry

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“…Nuclease digestion to 3‘-mononucleotides thus positions the label on the 5‘-residue adjacent to the original dNTP, and the 32 P-labeled nucleotide is then identified using 2D-TLC. The method was later expanded to analysis of RNA and found application in tRNA studies, where it was used in conjunction with the conserved structures and locations of many nucleotide modifications to derive tRNA sequences from patterns of nearest neighbors (e.g., refs and ). Although this approach could in principle be applied to contemporary problems of characterizing chemically synthesized DNA or RNA, the practicality of the method is limited by several factors.…”

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confidence: 99%

Determination of Nearest Neighbors in Nucleic Acids by Mass Spectrometry

Rozenski

McCloskey

1999

Anal. Chem.

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The identification of nearest-neighbor residues in nucleic acids provides useful constraints on establishment of base composition and sequence and is potentially applicable to a range of structural problems involving synthetic and natural polynucleotides. A new approach to this problem using electrospray ionization tandem mass spectrometry is based on measurement of precursor-product relationships derived from small fragment ions produced in the high-pressure ionization ("nozzle-skimmer") region of the instrument. Measured mass values of dinucleotide or other fragments, which give rise to mononucleotide ions N formed in the collision cell and transmitted by the second mass analyzer, establish the identities of residues adjacent to N. The technique is applicable to RNA and DNA, whether modified, or not, and is demonstrated using modified residues in nucleic acids up to the size of intact tRNA (76-mer). By monitoring of selected ion reaction channels, the method has been extended to LC/MS and to nearest-neighbor determinations directly in oligonucleotide mixtures.

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Reduced misreading of asparagine codons by Escherichia coli tRNA Lys with hypomodified derivatives of 5-methylaminomethyl-2-thiouridine in the wobble position 1 1Edited by D. E. Draper

Hagervall

Pomerantz

McCloskey

1998

Journal of Molecular Biology

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Nucleotide sequence of an arginine transfer ribonucleic acid from bacteriophage T4.

Cited by 22 publications

References 19 publications

Rare transfer ribonucleic acid essential for phage growth. Nucleotide sequence comparison of normal and mutant T4 isoleucine-accepting transfer ribonucleic acid

Rare transfer ribonucleic acid essential for phage growth. Nucleotide sequence comparison of normal and mutant T4 isoleucine-accepting transfer ribonucleic acid

Determination of Nearest Neighbors in Nucleic Acids by Mass Spectrometry

Reduced misreading of asparagine codons by Escherichia coli tRNA Lys with hypomodified derivatives of 5-methylaminomethyl-2-thiouridine in the wobble position 1 1Edited by D. E. Draper

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