Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus

Immunocytochemical characterization of p24, a baculovirus capsid-associated protein

“…These data, in combination with previous analyses of baculovirus structure, suggest that baculovirus capsids are composed of at least three components. These include p24, p39 (Pearson et al, 1988;Blissard et al, 1989;Thiem & Miller, 1989) and p87 (Mfiller et al, 1990;Lu & Carstens, 1991). Although the data presented in this report indicate that p24 is localized to the virion capsid, p24 was previously shown to be interrupted by a transposable element in the E strain of AcMNPV (Gombart et al, 1989a;Schetter et al, 1990).…”

“…The p39 protein is a major capsid component and has been described from three NPVs (Pearson et al, 1988;Blissard et al, 1989;Thiem & Miller, 1989;Bjornson & Rohrmann, 1992b). In addition, another protein, p87, appears to be present in both Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) and Orgyia pseudotsugata MNPV capsids (Miiller et aI., 1990;Lu & Carstens, 1991). In contrast, recent investigations by Kuzio et al (1989) suggest that a protein called p74 may be a specific component of PDV nucleocapsids that is required for infectivity in insects but not cultured cells.…”

Characterization of the interaction between the baculovirus ssDNA-binding protein (LEF-3) and putative helicase (P143).

“…A DNA polymerase activity differing from the cellular enzyme has been described in baculovirus-infected cells (Mikhailov et al, 1986 a, b ;Miller et al, 1981 ; and is probably attributable to the DNA polymerase homologue. In addition, a temperaturesensitive mutation in p143 (helicase gene) was shown to block DNA replication (Gordon & Carstens, 1984 ;Lu & Carstens, 1991).…”

“…Six of these genes appear to be required for DNA replication and encode the following proteins : a DNA polymerase homologue (Tomalski et al, 1988) ; P143, a helicase homologue (Lu & Carstens, 1991) that is capable of binding dsDNA (Laufs et al, 1997) ; LEF-3, a homotrimeric ssDNA-binding (SSB) protein Hang et al, 1995) that appears to be involved in transporting P143 into the nucleus (Wu & Carstens, 1998) ; IE-1, a transcriptional activator (Guarino & Summers, 1987) which also binds to hrs (Choi & Guarino, 1995 ;Guarino & Dong, 1991 ;Kovacs et al, 1992 ;Leisy et al, 1995 ;Rodems & Friesen, 1995) ; and two proteins, LEF-1 (a putative primase) and LEF-2, which have been shown to interact with each other . The three gene products, which are stimulatory for DNA replication, include two additional transcriptional activators, IE-2 (Carson et al, 1991) and PE-38 (Krappa & Knebel-Mo$ rsdorf, 1991), and P35, which blocks apoptosis (Clem et al, 1991 ;Herschberger et al, 1992) and may therefore influence DNA replication in an indirect manner.…”

Molecular biology of baculovirus and its use in biological control in Brazil

“…Vários genes essenciais à replicação do DNA foram identificados. Dados de seqüenciamento mostraram que um gene aná-logo ao DNA da polimerase foi identificado em AcNPV, BmNPV e CfNPV (Tomalski et al, 1988;Chaeychomsri et al, 1995;Liu & Carstens, 1995) e um gene análogo à helicase no AcNPV (Lu & Carstens, 1991). Estudos recentes mostraram que os genes ie-1, lef1, lef2 e lef3 também são essenciais à replicação do DNA de AcNPV e OpNPV (Kool et al, 1994;Ahrens et al, 1995;Lu & Miller, 1995).…”