Nucleotide sequence neighbouring a late modified guanylic residue within the 28S ribosomal RNA of several eukaryotic cells

Eladari, Marthe-Elisabeth; Hampe, A; Galibert, Francis

doi:10.1093/nar/4.6.1759

Cited by 17 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). We also believe that a (Table 3) and mouse (19) sequences; the yeast mitochondrial hairpin was derived from the sequence of Dujon (22); the E. coli hairpin from the sequence of Brosius et al (25); and the vertebrate hairpin from the sequence of a T1 oligonucleotide found in human, rat, mouse, and chicken 28S RNA (27). Kethoxal-reactive and puromycin-reactive nucleotides are marked with "k" and "+", respectively.…”

Section: ) Lsumentioning

confidence: 99%

“…With these data the positions of the methylated nucleosides within 13S and 17S rRNAs can be localized by examining the primary sequences of hamster and mouse mitochondrial rRNAs (14,19). Comparisons with related sequences from other mitochondrial rRNAs (20)(21)(22) and from bacterial (23)(24)(25), eucaryotic (26,27) and chloroplast (28,29) rRNAs underscore the conserved nature of these methylated rRNA regions. In addition, we propose that the UmGmU region of 17S rRNA comprises part of the ribosomal binding site for the 3'-terminus of aminoacyl-tRNA.…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates

Baer¹,

Dubin²

1981

Nucl Acids Res

View full text Add to dashboard Cite

The positions of post-transcriptionally methylated residues within hamster mitochondrial ribosomal RNA have been established. Comparisons with other mitochondrial rRNA, and with bacterial, eucaryotic and chloroplast rRNA show that the methylated regions i) are comprised of conserved primary sequences and/or secondary structures and ii) are situated at the subunit interface of the ribosome. The comparative analyses also reveal that the ribose-methylated sequence UmGmU of hamster mitochondrial large ribosomal subunit (LSU1) RNA lies in a universally conserved hairpin loop which contains a putative puromycin-reactive nucleotide. The "UmGmU hairpin" is within 100 nucleotides of two chloramphenicol-resistance residues of LSU RNA. We present a secondary structure for this region which is conserved in LSU RNAs. This structure allows physical juxtaposition of the three antibiotic-interacting loci and thus defines RNA components of the ribosomal-binding site for the 3'-terminus of aminoacyl-tRNA.

show abstract

Section: ) Lsumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates

Baer¹,

Dubin²

1981

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…In eukaryotes, these methylations are largely restricted to the 2'-0 position of the ribose moieties (1) and usually occur shortly after transcription, even before the initial transcript is cleaved into nucleolar intermediates or the mature RNA species (2)(3)(4). A few, however, particularly base methylations, such as the -m A -m5 Asequence in the 16S rRNA, are introduced into immediate precursors to the mature rRNA molecules (4)(5)(6) and at least one 2-0-methylation, in the 5.8S rRNA, occurs largely or entirely in the cytoplasm (7). Although their biological role is generally not understood, at least two effects have been advanced: a number of studies (8,9) suggest study on the -m6 A -m6 Asequence (10) indicates that these methylations destabilize the secondary structure of 16S rRNA in Escherichia coli.…”

Section: Introductionmentioning

confidence: 99%

Effect of 2′-O-methylation on the structure of mammalian 5.8S rRNAs and the 5.8S—28S rRNA junction

Nazar

Wildeman

et al. 1983

Nucl Acids Res

View full text Add to dashboard Cite

The mammalian 5.8S rRNA contains a partially 2'-O-methylated uridylic acid residue at position 14 which is largely or entirely methylated in the cytoplasm (Nazar, R.N., Sitz, T.O. and Sommers, K.D. (1980) J. Mol. Biol. 142, 117-121). The effect of this methylation on the 5.8S RNA structure and 5.8-28S rRNA junction was investigated using both chemical and physical approaches. Electrophoretic studies indicated that the free 5.8S rRNA can take on at least two different conformations and that the 2'-O-methylation at U14 restricts the molecule to the more hydrodynamically open form. Structural studies using limited pancreatic or T1 ribonuclease digestion indicated that the methylated conformation was more susceptible to digestion, consistent with a more open tertiary structure. Modification-exclusion studies indicated that the first 29 nucleotides at the 5' end and residues 140 through 158 at the 3' end affect the 5.8S-28S rRNA interaction, supporting previous suggestions that the 5.8S RNA interacts with its cognate high molecular weight component through its termini. These results also suggested that the 2'-O-methylated uridylic acid residue plays a role in the 5.8S-28S rRNA interaction and thermal denaturation studies confirmed this by showing that methylation destabilizes the 5.8S-28S rRNA junction. The 5.8-28S rRNA interaction appears to be more complex than previously believed.

show abstract

Chemical components

Bielka¹

1982

The Eukaryotic Ribosome

View full text Add to dashboard Cite

Nucleotide sequence neighbouring a late modified guanylic residue within the 28S ribosomal RNA of several eukaryotic cells

Cited by 17 publications

References 12 publications

Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates

Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates

Effect of 2′-O-methylation on the structure of mammalian 5.8S rRNAs and the 5.8S—28S rRNA junction

Chemical components

Contact Info

Product

Resources

About