Nucleotide sequence evidence for the occurrence of three distinct whitefly-transmitted, Sida-infecting bipartite geminiviruses in Central America.

Frischmuth, Thomas; Engel, Margit; Lauster, Susanne; Jeske, Holger

doi:10.1099/0022-1317-78-10-2675

Cited by 57 publications

(19 citation statements)

References 31 publications

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“…More importantly, the results herein suggest that begomoviruses may undergo greater-than-expected interspecific reassortment and leave open the possibility that reassortment may frequently be employed to modulate virulence and host range shifts. Reports of experimental (8,10,11,20,21,24,27) and natural (10,29,41) predicted reassortment in begomoviruses underscore the growing recognition of this genetic mechanism in host range shifting and modulated virulence in diversification. Indeed, there is no evidence for "cross-protection" (silencing) between begomoviruses, and they are commonly reported to establish mixed infections, even to the extent of occupying the same nucleus (39), all scenarios that would be expected to facilitate reassortment-based selection (3).…”

Section: Discussionmentioning

confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at ϳ90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.

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Section: Discussionmentioning

confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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“…Presently, 10 different begomovirus species have been isolated from Sida plants [17][18][19][20][21], of which nine were isolated from America and one from China. In this study we characterized, at the molecular level, genomic components of a new disease complex isolated from samples of Sida cordifolia in Hainan province, China.…”

Section: Discussionmentioning

confidence: 99%

Glucose signaling controls the transcription of retrotransposon Ty2–917 in Saccharomyces cerevisiae

Guo

Zhou

2007

Virus Genes

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Two virus isolates Hn57 and Hn60 were obtained from Sida cordifolia showing mild upward leaf-curling symptoms in Hainan province of China. Comparison of partial sequences of DNA-A like molecule confirmed the existence of a single type of begomovirus. The complete nucleotide sequence of DNA-A of Hn57 was determined to be 2757 nucleotides, with a genomic organization typical of begomoviruses. Complete sequence comparison with other reported begomoviruses revealed that Hn57 DNA-A has the highest sequence identity (71.0%) with that of Tobacco leaf curl Yunnan virus. Consequently, Hn57 was considered to be a new begomovirus species, for which the name Sida leaf curl virus (SiLCV) is proposed. In addition to DNA-A molecule, two additional circular single-stranded satellite DNA molecules corresponding to DNAbeta and DNA1 were found to be associated with SiLCV isolates. Both DNAbeta and DNA1 were approximately half the size of their cognate genomic DNA. Sequence analysis shows that DNAbeta of Hn57 and Hn60 share 93.8% nucleotide sequence identity, and they have the highest sequence identity (58.5%) with DNAbeta associated with Ageratum leaf curl disease (AJ316027). The nucleotide sequence identity between DNA1 of Hn57 and that of Hn60 was 83.8%, they share 58.2-79.3% nucleotide sequence identities in comparison with other previously reported DNAl.

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“…[10,11], while the begomovirus species Tomato yellow leaf curl virus, Calopogonium golden mosaic virus, Macroptilium yellow mosaic Florida virus, and Macroptilium mosaic Puerto Rico virus have members that naturally infect both wild and cultivated plants [12][13][14]. Plant species that are alternate hosts of crop-infecting begomoviruses during crop-free periods, become sources of inoculum, causing disease outbreaks and epidemics in cropping fields [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and experimental host range of an isolate of Wissadula golden mosaic St. Thomas virus

et al. 2009

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Partial genome segments of a begomovirus were previously amplified from Wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of St. Thomas, Jamaica and this isolate assigned to a tentative begomovirus species, Wissadula golden mosaic St. Thomas virus. To clone the complete genome of this isolate of Wissadula golden mosaic St. Thomas virus, abutting primers were designed to PCR amplify its full-length DNA-A and DNA-B components. Sequence analysis of the complete begomovirus genome obtained, confirmed that it belongs to a distinct begomovirus species and this isolate was named Wissadula golden mosaic St. Thomas virus-[Jamaica:Albion:2005] (WGMSTV-[JM:Alb:05]). The genome of WGMSTV-[JM:Alb:05] is organized similar to that of other bipartite Western Hemisphere begomoviruses. Phylogenetic analyses placed the genome components of WGMSTV-[JM:Alb:05] in the Abutilon mosaic virus clade and showed that the DNA-A component is most closely related to four begomovirus species from Cuba, Tobacco leaf curl Cuba virus, Tobacco leaf rugose virus, Tobacco mottle leaf curl virus, and Tomato yellow distortion leaf virus. The putative Rep-binding-site motif in the common region of WGMSTV-[JM:Alb:05] was observed to be identical to that of Chino del tomate virus-Tomato [Mexico:Sinaloa:1983], Sida yellow mosaic Yucatan virus-[Mexico:Yucatan:2005], and Tomato leaf curl Sinaloa virus-[Nicaragua:Santa Lucia], suggesting that WGMSTV-[JM:Alb:05] is capable of forming viable pseudo-recombinants with these begomoviruses, but not with other members of the Abutilon mosaic virus clade. Biolistic inoculation of test plant species with partial dimers of the WGMSTV-[JM:Alb:05] DNA-A and DNA-B components showed that the virus was infectious to Nicotiana benthamiana and W. amplissima and the cultivated species Phaseolus vulgaris (kidney bean) and Lycopersicon esculentum (tomato). Infected W. amplissima plants developed symptoms similar to symptoms observed under field conditions, confirming that this virus is a causal agent of Wissadula yellow mosaic disease in W. amplissima.

show abstract

Nucleotide sequence evidence for the occurrence of three distinct whitefly-transmitted, Sida-infecting bipartite geminiviruses in Central America.

Cited by 57 publications

References 31 publications

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Glucose signaling controls the transcription of retrotransposon Ty2–917 in Saccharomyces cerevisiae

Molecular characterization and experimental host range of an isolate of Wissadula golden mosaic St. Thomas virus

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