Nucleotide sequence and LexA regulation of the<i>Escherichia coli recN</i>gene

Rostas, Katalin; Morton, Stuart J.; Picksley, Steven M.; Lloyd, Robert G.

doi:10.1093/nar/15.13.5041

Cited by 58 publications

(29 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a function has been suggested for the S. cerevisiae Rad50 protein, which, although not having all the features of the SMC family, has several properties in common with Rad18 (Table 1). The conserved head and tail structures in Rad18 also show substantial sequence similarity to the E. coli RecN protein (40) (Fig. 9c and d), which is required for repair of double-strand breaks (34).…”

Section: Discussionmentioning

confidence: 91%

The rad18 Gene of Schizosaccharomyces pombe Defines a New Subgroup of the SMC Superfamily Involved in DNA Repair

Lehmann

Walicka

Griffiths

et al. 1995

Molecular and Cellular Biology

201

255

View full text Add to dashboard Cite

The rad18 mutant of Schizosaccharomyces pombe is very sensitive to killing by both UV and ␥ radiation. We have cloned and sequenced the rad18 gene and isolated and sequenced its homolog from Saccharomyces cerevisiae, designated RHC18. The predicted Rad18 protein has all the structural properties characteristic of the SMC family of proteins, suggesting a motor function-the first implicated in DNA repair. Gene deletion shows that both rad18 and RHC18 are essential for proliferation. Genetic and biochemical analyses suggest that the product of the rad18 gene acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair. This second repair pathway involves the products of the rhp51 gene (the homolog of the RAD51 gene of S. cerevisiae) and the rad2 gene.Cells of all organisms have evolved an intricate series of DNA repair pathways to counteract the deleterious effects of all types of DNA damage. In Escherichia coli, nucleotide excision repair (NER) of UV damage requires the products of six genes. A complex of the UvrA and UvrB proteins binds to DNA and translocates to the site of the damage. The UvrC product then attaches to the complex, displacing UvrA, and the damaged DNA strand is nicked on both sides of the damaged site. The helicase activity of the UvrD product releases the oligonucleotide containing the damage, and DNA polymerase I and ligase complete the repair process (24). In eukaryotes, NER requires considerably more gene products, most of which are highly conserved (20). In Saccharomyces cerevisiae, the products of the RAD1, -2, -3, -4, -10, -14, and -25 genes are absolutely required for excision repair of UV damage, whereas there is only a partial requirement for RAD7, -16, and -23. There is evidence that the products of many of these genes form a multisubunit complex (e.g., see reference 53). In Schizosaccharomyces pombe, genes encoding highly homologous proteins have been identified (9,10,30,39), demonstrating the conservation of the classical NER pathway in this yeast.Null mutations in the S. cerevisiae NER genes RAD1, -2, -3, -10, and -14 result in a total deficiency in excision repair of UV-induced cyclobutane dimers and 6-4 photoproducts (28). Null mutants of the S. pombe homologs of RAD1 and RAD2 (rad16 and rad13, respectively), while showing many properties expected of excision repair-deficient mutants, are still able to excise UV-induced cyclobutane dimers and 6-4 photoproducts at a significant rate (7, 27). These results suggest that, in contrast to S. cerevisiae, there is a second pathway in S. pombe for removal of UV photoproducts.Most of the rad mutants of S. pombe are sensitive to both UV and ␥ irradiation. Some of these are involved in checkpoint control of the cell cycle to radiation (2, 3, 41). Others, which are particularly sensitive to ionizing radiation, are deficient in recombination repair (6,29,33,54). No mutant which is sensitive to ionizing but not to UV irradiation has yet been identified. The S. pombe rad18-X mutant ...

show abstract

Section: Discussionmentioning

confidence: 91%

The rad18 Gene of Schizosaccharomyces pombe Defines a New Subgroup of the SMC Superfamily Involved in DNA Repair

Lehmann

Walicka

Griffiths

et al. 1995

Molecular and Cellular Biology

201

255

View full text Add to dashboard Cite

show abstract

“…The most promising similarity was found with the RecN proteins from Bacillus subtilis and Escherichia coli (Fig. 2a) (Rostas et al 1987;Van Hoy and Hoch 1990). These proteins also have a putative NTP-binding site as well as an acidic cluster with limited similarity to the DA box, separated by a domain with weak similarity to a coiled-coil (data not shown).…”

Section: Smc2 Product Is a New Member Of The Smc Familymentioning

confidence: 90%

SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.

Strunnikov¹,

Hogan²,

Koshland³

1995

Genes Dev.

330

303

View full text Add to dashboard Cite

We characterized the SMC2 (structural maintenance of chromosomes) gene that encodes a new Saccharomyces cerevisiae member of the growing family of SMC proteins. This family of evolutionary conserved proteins was introduced with identification of SMC1, a gene essential for chromosome segregation in budding yeast. The analysis of the putative structure of the Smc2 protein (Smc2p} suggests that it defines a distinct subgroup within the SMC family. This subgroup includes the ScII, XCAPE, and cutl4 proteins characterized concurrently. Smc2p is a nuclear, 135-kD protein that is essential for vegetative growth. The temperature-sensitive mutation, smc2-6, confers a defect in chromosome segregation and causes partial chromosome decondensation in cells arrested in mitosis. The Smc2p molecules are able to form complexes in vivo both with Smclp and with themselves, suggesting that they can assemble into a multimeric structure. In this study we present the first evidence that two proteins belonging to two different subgroups within the SMC family carry nonredundant biological functions. Based on genetic, biochemical, and evolutionary data we propose that the SMC family is a group of prokaryotic and eukaryotic chromosomal proteins that are likely to be one of the key components in establishing the ordered structure of chromosomes.

show abstract

“…Log phase cultures were treated with MMC (0.5 g/ml), and aliquots were taken at multiple time points for immunoblot analysis with anti-LexA (BioAcademia), anti-RecA (BioAcademia), or antiRecN (26). LexA resynthesis was inhibited by adding chloramphenicol (100 g/ml) to the cultures 10 min before adding MMC.…”

Section: Methodsmentioning

confidence: 99%

RecA Protein Recruits Structural Maintenance of Chromosomes (SMC)-like RecN Protein to DNA Double-strand Breaks

Keyamura

Sakaguchi

Kubota

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:RecN is an SMC (structural maintenance of chromosomes) family protein that is required for DNA doublestrand breaks (DSBs) repair. Results: We identified a RecA mutant that is deficient in interacting with RecN. Conclusion: A functional interaction between RecN and RecA is required for assembly of RecN at the sites of DSBs. Significance: RecN is critical for protecting the structural integrity of chromosomes during DSBs repair.

show abstract

Nucleotide sequence and LexA regulation of theEscherichia coli recNgene

Cited by 58 publications

References 36 publications

The rad18 Gene of Schizosaccharomyces pombe Defines a New Subgroup of the SMC Superfamily Involved in DNA Repair

The rad18 Gene of Schizosaccharomyces pombe Defines a New Subgroup of the SMC Superfamily Involved in DNA Repair

SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.

RecA Protein Recruits Structural Maintenance of Chromosomes (SMC)-like RecN Protein to DNA Double-strand Breaks

Contact Info

Product

Resources

About