Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen
            <i>Pseudomonas syringae</i>
            pv. maculicola ES4326

Stavrinides, John; Guttman, David S.

doi:10.1128/jb.186.15.5101-5115.2004

Cited by 49 publications

(77 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and only 2 out of the 11 P. syringae plasmids already sequenced (pPMA4326D and pPMA4326E from P. syringae pv. maculicola strain ES4326) are n-PFPs (36). The role in virulence of these two plasmids, which contain only six ORFs, each of them encoding unknown hypothetical proteins, remains unknown thus far (36).…”

Section: Discussionmentioning

confidence: 99%

“…Due to the importance of PFPs to the biology of P. syringae, several P. syringae plasmids have been sequenced recently in order to understand their role in pathogenesis as well as their origin, evolution, and coexistence-they use the same replicon-within the same strain (4,22,31,36,39). These genomic resources and previous experimental data have enabled comparative analyses of the gene content of PFPs from different pathovars of P. syringae.…”

mentioning

confidence: 99%

“…During the last decade, research on diseases caused by pseudomonads in herbaceous plants has progressed rapidly, and the application of molecular genetics and genome and plasmid sequencing (4,13,22,31,36,39) has provided new insights into determinants of pathogenicity and virulence. However, there is a general lack of knowledge on virulence and pathogenicity determinants specific for infection of woody plants.…”

mentioning

confidence: 99%

“…Most native plasmids of P. syringae and related pathogens have been shown to belong to the pPT23A family, a phylogenetically related plasmid group characterized by the ubiquitous presence of a replication region containing the repA gene (15,27,37,44). pPT23A family plasmids (PFPs) usually encode determinants related to virulence and pathogenicity, such as genes involved in the biosynthesis of the phytotoxin coronatine (2), effector proteins (31,36,43), and fitness determinants conferring resistance to copper and antibiotics as well as tolerance to UV radiation (3,38,40). The role of PFPs in pathogen virulence and growth in their specific hosts has been demonstrated using curing or disruption of some of these determinants in several P. syringae pathosystems (20,26,44).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids

et al. 2008

View full text Add to dashboard Cite

Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of information about the gene content of P. savastanoi pv. savastanoi plasmids and their role in the interaction of this pathogen with olive plants. We designed a DNA macroarray containing 135 plasmid-borne P. syringae genes to conduct a global genetic analysis of 32 plasmids obtained from 10 P. savastanoi pv. savastanoi strains. Hybridization results revealed that the number of PFPs per strain varied from one to four. Additionally, most strains contained at least one plasmid (designated non-PFP) that did not hybridize to the repA gene of pPT23A. Only three PFPs contained genes involved in the biosynthesis of the virulence factor indole-3-acetic acid (iaaM, iaaH, and iaaL). In contrast, ptz, a gene involved in the biosynthesis of cytokinins, was found in five PFPs and one non-PFP. Genes encoding a type IV secretion system (T4SS), type IVA, were found in both PFPs and non-PFPs; however, type IVB genes were found only on PFPs. Nine plasmids encoded both T4SSs, whereas seven other plasmids carried none of these genes. Most PFPs and non-PFPs hybridized to at least one putative type III secretion system effector gene and to a variety of additional genes encoding known P. syringae virulence factors and one or more insertion sequence transposase genes. These results indicate that non-PFPs may contribute to the virulence and fitness of the P. savastanoi pv. savastanoi host. The overall gene content of P. savastanoi pv. savastanoi plasmids, with their repeated information, mosaic arrangement, and insertion sequences, suggests a possible role in adaptation to a changing environment.Pseudomonas syringae strains and related pathogens, such as Pseudomonas savastanoi strains, infect a wide range of herbaceous and woody plants causing diverse symptoms, such as leaf spots and blights, soft rots of fruits, wilts, overgrowths, scabs, and cankers (18,23). During the last decade, research on diseases caused by pseudomonads in herbaceous plants has progressed rapidly, and the application of molecular genetics and genome and plasmid sequencing (4,13,22,31,36,39) has provided new insights into determinants of pathogenicity and virulence. However, there is a general lack of knowledge on virulence and pathogenicity determinants specific for infection of woody plants. Clear examples of this are the pathogens P. savastanoi pathovars nerii, fraxini, and savastanoi, which cause knots and galls on members of the various genera of the family Oleaceae and oleander (14). Symptoms on infected trees include hyperplasia formation on stems and branches and occasionally on leaves and fruit (23). At present, the only P. savastanoi determinants known to be involved in knot development are production of the phytohormones indole-3-acetic acid (IAA) an...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Recombination has been described as one of the most frequent diversification mechanisms in begomoviruses (Padidam et al, 1999;Monci et al, 2002;Preiss & Jeske, 2003;Morilla et al, 2004, García-Andrés et al, 2006. This can be a rapid process to create new genomes with adaptive advantages, which could accelerate their evolution and favour the expansion of the host range and, therefore, the emergence of novel diseases (Lively & Dybdahl, 2000;Dybdahl & Storfer, 2003;Stavrinides & Guttman, 2004). Interspecific recombination has been widely described as a speciation mechanism in the genus Begomovirus.…”

Section: G Lozano and Others 2556mentioning

confidence: 99%

Novel begomovirus species of recombinant nature in sweet potato (Ipomoea batatas) and Ipomoea indica: taxonomic and phylogenetic implications

Lozano

Trenado

Valverde

et al. 2009

Journal of General Virology

View full text Add to dashboard Cite

Viral diseases occur wherever sweet potato (Ipomoea batatas) is cultivated and because this crop is vegetatively propagated, accumulation and perpetuation of viruses can become a major constraint for production. Up to 90 % reductions in yield have been reported in association with viral infections. About 20 officially accepted or tentative virus species have been found in sweet potato and other Ipomoea species. They include three species of begomoviruses (genus Begomovirus, family Geminiviridae) whose genomes have been fully sequenced. In this investigation, we conducted a search for begomoviruses infecting sweet potato and Ipomoea indica in Spain and characterized the complete genome of 15 isolates. In addition to sweet potato leaf curl virus (SPLCV) and Ipomoea yellowing vein virus, we identified three new begomovirus species and a novel strain of SPLCV. Our analysis also demonstrated that extensive recombination events have shaped the populations of Ipomoea-infecting begomoviruses in Spain. The increased complexity of the unique Ipomoea-infecting begomovirus group, highlighted by our results, open new horizons to understand the phylogeny and evolution of the family Geminiviridae. INTRODUCTIONSweet potato (Ipomoea batatas, family Convolvulaceae) is ranked as the seventh most important food crop in global production. This crop originated in South America (O'Brien, 1972) but today it is grown in most tropical, subtropical and temperate regions of the world. Numerous ornamental Ipomoea species are grown worldwide. Ipomoea indica is widely grown ornamentally in the Mediterranean basin, where it is frequently naturalized. The putative role of I. indica and other species in the genus as a reservoir for sweet potato pathogens has not been explored.Because sweet potato is vegetatively propagated, accumulation of viruses can become a major constraint for production. About 20 accepted or tentative virus species have been described in sweet potato . Three begomovirus species have been described as infecting Ipomoea species and their genomes have been fully sequenced. These are: sweet potato leaf curl virus (SPLCV) (Lotrakul et al., 1998;Lotrakul & Valverde., 1999), Ipomoea yellow vein virus (IYVV) (Banks et al., 1999) and sweet potato leaf curl Georgia virus (SPLCGV) (Lotrakul et al., 2003). SPLCV or related begomoviruses have been isolated from sweet potato in the Americas (Lotrakul et al., 2003;Fuentes & Salazar, 2003), Asia (Onuki & Hanada, 1998;Luan et al., 2006) and Africa (Miano et al., 2006) and from I. indica in Europe . Begomoviruses are likely to be present in many regions where sweet potato is grown but their prevalence and distribution is still unknown. Difficulties in detecting and isolating begomoviruses directly from sweet potato plants could have impeded additional reports (Kokkinos & Clark., 2006b).Information about the variability among isolates of the described sweet potato begomoviruses is limited. Comparisons and phylogenetic analyses of partial sequences of the AC1 gene of several isolates collect...

show abstract

Comparative genomics ofPseudomonas syringaepv.syringaestrains B301D andHS191 and insights into intrapathovar traits associated with plant pathogenesis

et al. 2015

View full text Add to dashboard Cite

Pseudomonas syringae pv. syringae is a common plant-associated bacterium that causes diseases of both monocot and dicot plants worldwide. To help delineate traits critical to adaptation and survival in the plant environment, we generated complete genome sequences of P. syringae pv. syringae strains B301D and HS191, which represent dicot and monocot strains with distinct host specificities. Intrapathovar comparisons of the B301D (6.09 Mb) and HS191 (5.95 Mb plus a 52 kb pCG131 plasmid) genomes to the previously sequenced B728a genome demonstrated that the shared genes encompass about 83% of each genome, and include genes for siderophore biosynthesis, osmotolerance, and extracellular polysaccharide production. Between 7% and 12% of the genes are unique among the genomes, and most of the unique gene regions carry transposons, phage elements, or IS elements associated with horizontal gene transfer. Differences are observed in the type III effector composition for the three strains that likely influences host range. The HS191 genome had the largest number at 25 of effector genes, and seven effector genes are specific to this monocot strain. Toxin production is another major trait associated with virulence of P. syringae pv. syringae, and HS191 is distinguished by genes for production of syringopeptin SP25 and mangotoxin.

show abstract

Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

Cited by 49 publications

References 86 publications

Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids

Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids

Novel begomovirus species of recombinant nature in sweet potato (Ipomoea batatas) and Ipomoea indica: taxonomic and phylogenetic implications

Comparative genomics ofPseudomonas syringaepv.syringaestrains B301D andHS191 and insights into intrapathovar traits associated with plant pathogenesis

Contact Info

Product

Resources

About