Nucleotide Sequence Analysis of the Lactococcal EPS Plasmid pNZ4000

Kranenburg, Richard van; Kleerebezem, Michiel; Vos, Willem M. de

doi:10.1006/plas.1999.1453

Cited by 50 publications

(29 citation statements)

References 31 publications

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“…Moreover, it has been suggested that, despite the high homology between their iterons, the interaction between the replication origin and the replication protein RepB is highly specific, in many cases rendering the plasmids compatible (34). Indeed, four functional and compatible theta-type replicons were identified on pNZ4000 (67,68), and at least four of the six replicons on the four SK11 plasmids were found to be compatible with each other.…”

Section: Resultsmentioning

confidence: 99%

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Complete Sequences of Four Plasmids ofLactococcus lactissubsp.cremorisSK11 Reveal Extensive Adaptation to the Dairy Environment

Siezen¹,

Renckens²,

Swam

et al. 2005

Appl Environ Microbiol

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Lactococcus lactis strains are known to carry plasmids encoding industrially important traits. L. lactis subsp. cremoris SK11 is widely used by the dairy industry in cheese making. Its complete plasmid complement was sequenced and found to contain the plasmids pSK11A (10,372 bp), pSK11B (13,332 bp), pSK11L (47,165 bp), and pSK11P (75,814 bp). Six highly homologous repB-containing replicons were found, all belonging to the family of lactococcal theta-type replicons. Twenty-three complete insertion sequence elements segment the plasmids into numerous modules, many of which can be identified as functional units or containing functionally related genes. Plasmid-encoded functions previously known to reside on L. lactis SK11 plasmids were now mapped in detail, e.g., lactose utilization (lacR-lacABCDFEGX), the proteolytic system (prtM-prtP, pepO, pepF), and the oligopeptide permease system (oppDFBCA). Newly identified plasmid-encoded functions could facilitate the uptake of various cations, while the pabA and pabB genes could be essential for folate biosynthesis. A competitive advantage could be obtained by using the putative flavin adenine dinucleotide-dependent D-lactate dehydrogenase and oxalate:formate antiporter for enhanced ATP synthesis, while the activity of the predicted ␣-acetolactate decarboxylase may contribute to the formation of an additional electron sink. Various stress response proteins are plasmid encoded, which could enhance strain robustness. A substantial number of these "adaptation" genes have not been described before on L. lactis plasmids. Moreover, several genes were identified for the first time in L. lactis, possibly reflecting horizontal gene transfer.

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Section: Resultsmentioning

confidence: 99%

“…The 42.8-kb mobilization plasmid pNZ4000 from L. lactis subsp. cremoris NIZO B40 has a module encoding exopolysaccharide biosynthesis but also encodes putative cobalt and magnesium transport systems (68).…”

mentioning

confidence: 99%

Complete Sequences of Four Plasmids ofLactococcus lactissubsp.cremorisSK11 Reveal Extensive Adaptation to the Dairy Environment

Siezen¹,

Renckens²,

Swam

et al. 2005

Appl Environ Microbiol

Self Cite

131

169

View full text Add to dashboard Cite

show abstract

“…Thus we concluded that this circular structure is a plasmid. In the genus Lactococcus it is known that some species possess a plasmid carrying a gene cluster for exopolysaccharide production, but such bacteria work as a starter culture of fermented dairy products (van Kranenburg et al, 2000;Forde and Fitzgerald, 2003;Knoshaug et al, 2007). The presence of plasmids carrying a gene cluster for exopolysaccharide production might be a common feature in Lactococcus.…”

Section: Discussionmentioning

confidence: 99%

A Novel Plasmid Carrying Capsule Gene Cluster Found in <i>Lactococcus garvieae</i> Isolated from Filefish

et al. 2017

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“…The hypothetical protein (HP) encoded by SGO_1244 has amino acid regions with significant similarity to the Abi family proteins (PF07751) involved in bacteriophage resistance mediated by abortive infection in lactococcal species (29). The fic gene of S. mutans, which despite its location has no homology to camG, encodes a putative mobilization protein similar to that in lactococcal plasmid pNZ4000 (58).…”

Section: Figmentioning

confidence: 99%

A Genetic Determinant in Streptococcus gordonii Challis Encodes a Peptide with Activity Similar to That of Enterococcal Sex Pheromone cAM373, Which Facilitates Intergeneric DNA Transfer

et al. 2010

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Enterococcus faecalis strains secrete multiple peptides representing different sex pheromones that induce mating responses by bacteria carrying specific conjugative plasmids. The pheromone cAM373, which induces a response by the enterococcal plasmid pAM373, has been of interest because a similar activity is also secreted by Streptococcus gordonii and Staphylococcus aureus. The potential to facilitate intergeneric DNA transfer from E. faecalis is of concern because of extensive multiple antibiotic resistance, including vancomycin resistance, that has emerged among enterococci in recent years. Here, we characterize the related pheromone determinant in S. gordonii and show that the peptide it encodes, gordonii-cAM373, does indeed induce transfer of plasmid DNA from E. faecalis into S. gordonii. The streptococcal determinant camG encodes a lipoprotein with a leader sequence, the last 7 residues of which represent the gordonii-cAM373 heptapeptide SVFILAA. Synthetic forms of the peptide had activity similar to that of the enterococcal cAM373 AIFILAS. The lipoprotein moiety bore no resemblance to the lipoprotein encoded by E. faecalis. We also identified determinants in S. gordonii encoding a signal peptidase and an Eep-like zinc metalloprotease (lspA and eep, respectively) similar to those involved in processing certain pheromone precursors in E. faecalis. Mutations generated in camG, lspA, and eep each resulted in the ablation of gordonii-cAM373 activity in culture supernatants. This is the first genetic analysis of a potential sex pheromone system in a commensal oral streptococcal species, which may have implications for intergeneric gene acquisition in oral biofilms.

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Nucleotide Sequence Analysis of the Lactococcal EPS Plasmid pNZ4000

Cited by 50 publications

References 31 publications

Complete Sequences of Four Plasmids ofLactococcus lactissubsp.cremorisSK11 Reveal Extensive Adaptation to the Dairy Environment

Complete Sequences of Four Plasmids ofLactococcus lactissubsp.cremorisSK11 Reveal Extensive Adaptation to the Dairy Environment

A Novel Plasmid Carrying Capsule Gene Cluster Found in <i>Lactococcus garvieae</i> Isolated from Filefish

A Genetic Determinant in Streptococcus gordonii Challis Encodes a Peptide with Activity Similar to That of Enterococcal Sex Pheromone cAM373, Which Facilitates Intergeneric DNA Transfer

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