Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes.

Abstract

In the above paper Fig. 1. The complete and correct bel 1 protein sequence of the human spumaretrovirus. Note that the corrected bel 1 protein has extensions at both the amino-and at the carboxy-terminus.

Molecular cloning of the genome of human spumaretrovirus

“…3). This interpretation was verified by nucleotide sequence analysis (Flügel et al, 1987). Similar mapping experiments and nucleotide sequence analyses confrrmed the restriction maps of all recombinant clones shown in Fig.…”

“…4C. The result was confrrmed by nucleotide sequence analysis which proved that the overlap regions encompassed 1225 and 651 nt (Flügel et al, 1987).…”

“…The molecular clones of HSRV DNA will facilitate further studies on the structure and function of HSRV genome and its gene products. HEL cells were grown as described (Flügel et al, 1987) and HSRV.was kindly provided by Dr. P. Loh (Loh and Ang, 1981 ). Virus was propagated by rnixing infected HEL cells with uninfected growing HEL cells at a ratio of 1: 4 (Neumann- Haefelin et al, 1983).…”

“…3) was cbaracterized in detail by restriction enzyme analysis and Southem blot. hybridizations (Flügel et al, 1987).…”

Intrabody-mediated knockout of the high-affinity IL-2 receptor in primary human T cells using a bicistronic lentivirus vector

“…pHCMV-G expressing the VSV G protein was provided by Dr T Friedmann. 19 A vector expressing the human foamy virus env gene was constructed by subcloning an XhoI to EcoRI fragment of the HFV genome 53 provided by Dr R Flugel into pRC/CMV (Invitrogen, Carlsbad, CA, USA).…”