Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families

Raschke, Eberhard; Baumann, Götz; Schöffl, Fritz

doi:10.1016/0022-2836(88)90300-2

Cited by 64 publications

(52 citation statements)

References 61 publications

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“…Preliminary observations in our laboratory (10) and other groups [15][16][17][18][19][20][21][22][23][24][25][26][27][28]29] indicated expression of LMW HSPs during zygotic embryogenesis and seed germination. The results presented in this paper demonstrate differential developmental expression of two distinct families of cytoplasmic proteins.…”

Section: Discussionmentioning

confidence: 99%

“…2A and data not shown). Conversely, sunflower HSP17.9 showed the highest homology (similarity 95.0~o; identity 63.5~/o) to proteins such as soybean HSP17.9D [24], belonging to class II (Fig. 2B).…”

Section: Dry-seed-stored L M W Hsp Mrnas Encode Putative Class I and mentioning

confidence: 99%

“…Its deduced amino acid sequence (Fig. 1) contains the characteristic motif GVLTV located within a conserved hydrophobic domain which is present in the carboxyterminal region of LMW HSPs ( [2,24]; see below).…”

Section: G a T G T T T G G T G T G T T A T G A G T T~g G G G T T T G mentioning

confidence: 99%

“…Most plant LMW H SP genes belong to conserved multigene families with complex expression patterns, which are result of the intrinsic genetic variability of these families, and (or) of diverse posttranslational modification of their encoded proteins [2,24]. In a previous paper, we showed that using antibodies against seed-stored HSP17.9, we could distinguish between the immuno-reacting polypeptides that were expressed either in response to heat shock, or in response to water stress in sunflower plants.…”

Section: Class H L M W Hsps Expressed During Seed Development In Sunfmentioning

confidence: 99%

“…In plants, experimental work on HSP function is still far for similar refinement [reviewed in 2]. If plant LMW HSPs were also molecular chaperones, as inferred from their structural similarity with animal proteins [2,24], such activity would be meaningful in any of the possible functional scenarios for HSP17.6 and HSP 17.9 discussed above. LMW HSPs present in dry seeds and young seedlings could be abundant (0.5-1.5 ~o of total proteins [ 15 ]).…”

Section: Potential Functions Of Sunflower L M W Hsps In Seed Developmentmentioning

confidence: 99%

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Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

1994

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We isolated and sequenced Ha hsp17.9, a DNA complementary (cDNA) of dry-seed stored mRNA that encodes a low-molecular-weight heat-shock protein (LMW HSP). Sequence analysis identified Ha hspl7.9, and the previously reported Ha hspl7.6, as cDNAs encoding proteins (HSP17.6 and H S P 17.9) which belong to different families of cytoplasmic LMW H S Ps. Using specific antibodies we observed differential expression of both proteins during zygotic embryogenesis under controlled environment, and a remarkable persistence of these LMW HSPs during germination. Immuno-blot analysis of HSP17.9 proteins in two-dimensional gels revealed that the polypeptides expressed in embryos were indistinguishable from LMW HSPs expressed in vegetative tissues in response to water deficit; but they appeared different from homologeous proteins expressed in response to thermal-stress. Tissue-print immunolocalization experiments showed that HSP17.9 and HSP17.6 were homogeneously distributed in every tissue of desiccation-tolerant dry seeds and young seedlings under non-stress conditions. These results demonstrate developmental regulation of specific, cytoplasmic, plant LMW HSPs, suggesting also their involvement in water-stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Dry-seed-stored L M W Hsp Mrnas Encode Putative Class I and mentioning

confidence: 99%

Section: G a T G T T T G G T G T G T T A T G A G T T~g G G G T T T G mentioning

confidence: 99%

Section: Class H L M W Hsps Expressed During Seed Development In Sunfmentioning

confidence: 99%

Section: Potential Functions Of Sunflower L M W Hsps In Seed Developmentmentioning

confidence: 99%

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Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

1994

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Constitutive transcription of a soybean heat‐shock gene by a cauliflower mosaic virus promoter in transgenic tobacco plants

1987

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Transcription of heat-shock protein genes in soybean can be induced by high temperature stress leading to a transient expression of heat-shock proteins. We have tested whether the replacement of a native heat-shock promoter by a viral promoter results in constitutive transcript levels of the respective gene in transgenic plants. The 35s-transcript promoter of the cauliflower mosaic virus was linked to the protein-coding region of the genomic heat-shock gene hs6831, encoding a 17.6-kD heat-shock protein of soybean. After transformation of tobacco plants with this chimeric construction using a disarmed Agrobacterium binary vector, abundant mRNA levels were detected in transgenic plants. The steadystate level of this mRNA at 25°C was equal to that generated by the native heat-shock promoter at 40°C; however, it was markedly reduced by heat shock applied to the transgenic plants. These findings suggest a sufficiently high stability of heat-shock mRNA produced at the normal growth temperature to direct constitutive expression of heat-shock proteins. The application of constitutive gene expression for the investigation of thermotolerance is discussed.

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The independent stage‐specific expression of the 18‐kDa heat shock protein genes during microsporogenesis in Zea mays L

et al. 1993

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The small (18-kDa) heat shock proteins (hsps) of maize are encoded by a complex multigene family. In a previous report, we described the genetic information from cDNAs encoding two different members of the family. In this communication, we report the isolation and characterization of cDNA and genomic clones encoding information for a third member of this hsp family (c/gMHSP18-1). DNA fragments containing nucleotide sequences common to, or specific for, each of these characterized 18-kDa genes were prepared and used as probes to assess the expression of these genes during microsporogenesis and development of the gametophyte in an inbred line of maize (Oh43). Our results demonstrate (1) that mRNA transcripts encoding the 18-kDa hsps are expressed and/or accumulate during microsporogenesis, and (2) that genes encoding two of the characterized 18-kDa hsps are expressed and/or accumulate independently, in a stage-specific manner during microsporogenesis. These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response.

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Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families

Cited by 64 publications

References 61 publications

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

Constitutive transcription of a soybean heat‐shock gene by a cauliflower mosaic virus promoter in transgenic tobacco plants

The independent stage‐specific expression of the 18‐kDa heat shock protein genes during microsporogenesis in Zea mays L

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