Nucleotide Sequence Analysis of pOg32, a Cryptic Plasmid fromLeuconostoc oenos

Brito, Luísa; Vieira, G.; Santos, Mário A.; Paveia, H.

doi:10.1006/plas.1996.0031

Cited by 21 publications

(8 citation statements)

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“…There has been only one report of a successful transformation of O. oeni and this involved the use of electroporation (Dicks 1994), however, it appears that no-one has been able to repeat this success and most workers in the field still regard O. oeni as being recalcitrant. Interestingly, O. oeni does harbour cryptic plasmids that have been sequenced and could potentially be modified to be used as vectors for transformation (Brito and Paveia 1999, Brito et al 1996, Janse et al 1987, Mesas et al 2001, Prévost et al 1995, Zúñiga et al 1996a), but until someone develops a method for DNA-uptake that works for this bacterium, the plasmids will continue to spend most of their time in the laboratory fridge.…”

Section: Genetic Manipulation Of Wine Bacteriamentioning

confidence: 99%

Oenococcus oeni and malolactic fermentation – moving into the molecular arena

Bartowsky

2005

Aust J Grape Wine Res

136

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Malolactic fermentation (MLF) is the bacterial‐driven decarboxylation of L‐malic acid to L‐lactic acid and carbon dioxide, and brings about deacidification, flavour modifications and microbial stability of wine. Pasteur first described the presence of ‘bacteria’ in wine nearly one‐hundred‐and‐fifty years ago and the subsequent elucidation of the bacterial‐driven malolactic reaction was described about fifty years later. Over the following years the occurrence of MLF became apparent in wines worldwide, and eventually Oenococcus oeni was identified as the principal organism involved in the process. O. oeni is remarkable in its ability to tolerate the nutritionally poor and challenging, harsh wine environment; however, it can be a difficult and sometimes unreliable organism to work with in the winery. A greater knowledge of its biology would undoubtedly facilitate the development of strains and practices, with improved performance outcomes. We already know a considerable amount about the biochemistry and physiology of O. oeni, and ironically, although we know little about its genetics, its genome has been sequenced. With this groundwork in place and molecular biology techniques at our disposal we are poised to increase our knowledge and understanding of this organism enormously.

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Section: Genetic Manipulation Of Wine Bacteriamentioning

confidence: 99%

Oenococcus oeni and malolactic fermentation – moving into the molecular arena

Bartowsky

2005

Aust J Grape Wine Res

136

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“…In the dairy starter Lactococcus lactis , they confer phenotypes that reflect adaptation to the dairy environment, such as lactose catabolism, protease activity, peptide and amino acid uptake and bacteriophage resistance [25]. Six small cryptic plasmids of O. oeni were sequenced and described to date: pLo13 [26], p4028 [27], pOg32 [28], pRS1 [29], pRS2 and pRS3 [30]. They encode replication and mobilization proteins but do not carry any gene potentially involved in wine adaptation.…”

Section: Introductionmentioning

confidence: 99%

Identification of pOENI-1 and Related Plasmids in Oenococcus oeni Strains Performing the Malolactic Fermentation in Wine

et al. 2012

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Plasmids in lactic acid bacteria occasionally confer adaptive advantages improving the growth and behaviour of their host cells. They are often associated to starter cultures used in the food industry and could be a signature of their superiority. Oenococcus oeni is the main lactic acid bacteria species encountered in wine. It performs the malolactic fermentation that occurs in most wines after alcoholic fermentation and contributes to their quality and stability. Industrial O. oeni starters may be used to better control malolactic fermentation. Starters are selected empirically by virtue of their fermentation kinetics and capacity to survive in wine. This study was initiated with the aim to determine whether O. oeni contains plasmids of technological interest. Screening of 11 starters and 33 laboratory strains revealed two closely related plasmids, named pOENI-1 (18.3-kb) and pOENI-1v2 (21.9-kb). Sequence analyses indicate that they use the theta mode of replication, carry genes of maintenance and replication and two genes possibly involved in wine adaptation encoding a predicted sulphite exporter (tauE) and a NADH:flavin oxidoreductase of the old yellow enzyme family (oye). Interestingly, pOENI-1 and pOENI-1v2 were detected only in four strains, but this included three industrial starters. PCR screenings also revealed that tauE is present in six of the 11 starters, being probably inserted in the chromosome of some strains. Microvinification assays performed using strains with and without plasmids did not disclose significant differences of survival in wine or fermentation kinetics. However, analyses of 95 wines at different phases of winemaking showed that strains carrying the plasmids or the genes tauE and oye were predominant during spontaneous malolactic fermentation. Taken together, the results revealed a family of related plasmids associated with industrial starters and indigenous strains performing spontaneous malolactic fermentation that possibly contribute to the technological performance of strains in wine.

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“…are a diverse group of heterofermentative LAB of considerable industrial importance. Many Leuconostoc species harbor one or more native plasmids of various sizes, but to date, only a few reports have dealt with RCR plasmids in species of the Leuconostoc genus (5,7,10,11,30) and none concerns identification of a non-RCR plasmid. The aim of the present study was to analyze the mode of replication of the Leuconostoc plasmid pTXL1 (6) in order to develop families of vectors designed for specific industrial applications.…”

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confidence: 99%

Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector

Biet

Cenatiempo

Fremaux³

2002

Appl Environ Microbiol

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A 2,665-bp cryptic plasmid, pTXL1, isolated from Leuconostoc mesenteroides subsp. mesenteroides Y110 was identified. This plasmid harbors a replicon localized on a 1,300-bp fragment. Two observations suggested that pTXL1 does not belong to rolling-circle replication (RCR)-type plasmids and most likely replicates via a theta mechanism. These hypotheses are supported by the observation that no detectable single-stranded intermediate was found for the replicon and that, unlike in RCR-type plasmids, the pTXL1 replicon sequence lacks an open reading frame encoding a replicase. The small-sized pTXL1 plasmid is stable and, according to its origin, can be considered in the "generally recognized as safe" category. Its ability to replicate in several lactic acid bacteria was exploited to develop a vector producing mesentericin Y105, a class II anti-Listeria bacteriocin. With this new vector, a recombinant industrial Leuconostoc cremoris strain able to produce mesentericin Y105 was constructed.

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Nucleotide Sequence Analysis of pOg32, a Cryptic Plasmid fromLeuconostoc oenos

Cited by 21 publications

References 0 publications

Oenococcus oeni and malolactic fermentation – moving into the molecular arena

Oenococcus oeni and malolactic fermentation – moving into the molecular arena

Identification of pOENI-1 and Related Plasmids in Oenococcus oeni Strains Performing the Malolactic Fermentation in Wine

Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector

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