Nucleotide preparation from cells and determination of nucleotides by ion-pair high-performance liquid chromatography

Analysis of nucleosides and monophosphate nucleotides from mushrooms with reversed‐phase HPLC

“…As a result of their ionic proper-ties, nucleotides strongly interact with cationic ion-pair reagents, if certain requirements are fulfilled, e.g. convenient pH [18][19][20][21]. The shortcomings of gradient elution in the presence of an ion-pair reagent, which cannot be neglected, include a prolonged equilibration time and a reduced column lifetime.…”

A Fluorometric Assay for Cyclic Guanosine 3′,5′-Monophosphate Incorporating a Sep-Pak Cartridge and Enzymatic Cycling

“…Currently, three available methods, radioimmunoassay (2), enzyme immunoassay (3), and high-performance liquid chromatography (4), are used to measure cGMP contents. Although the most sensitive method for assaying cGMP is a radioimmunoassay, whose detection limit is 1 fmol/sample, it has several problems.…”

High-performance anion-exchange chromatography using on-line electrolytic eluent generation for the determination of more than 25 intermediates from energy metabolism of mammalian cells in culture

“…Lu et al [26] also used perchloric acid on an AS11 column, however in atypically low concentrations of 0.3 mM compared to at least 0.1 M used by other experimenters, e.g. [6,15,28]. When testing the procedure from Lu et al [26] with our samples, no protein was precipitated, which made this method not applicable.…”

“…Probably the most often applied extraction method for intracellular metabolites in literature uses perchloric acid [6,8,15,26,28]. The major drawback of this method in combination with anion-exchange chromatography is the contamination of samples with perchlorate ions, which cannot be removed completely by neutralisation with, e.g.…”

“…More recent studies were done on, e.g. Lactococcus lactis [4], carcinoma cells [5,6], various normal and tumor cell lines [7], and epithelial cell lines [8].…”