Nucleotide Metabolism

Hurlbert, Robert B.; Schmitz, Hanns; Brumm, Anne F.; Potter, Van R.

doi:10.1016/s0021-9258(18)65528-0

Cited by 941 publications

(26 citation statements)

References 18 publications

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“…Ion exchange chromatography was carried out as described by Hurlbert et al (1954) and by with the indicated modifications to effect the separations on a gram scale. The chr ornato graphic fractions were analyzed with the suitable dehydrogenase reaction mixtures as well as by their absorbancy at 260 µ; the pooled tubes were precipitated as described by Kaplan and Ciotti (1956) by adjustment of pH, precipitation with cold acetone, and washing with acetone and ether.…”

Section: Methodsmentioning

confidence: 99%

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis^*

Stein¹,

Lee²,

Anderson³

et al. 1963

Biochemistry

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The synthesis of the thionicotinamide analog of triphosphopyridine nucleotide catalyzed by pig brain transglycosidase is described. The thionicotinamide analogs of both diphospho-and triphosphopyridine nucleotide have been purified from the corresponding nicotinamide nucleotides by preparative gradient elution chromatography, and characterized by elementary analyses. The spectrophotometric constants reported are based on ribose, phosphate, and adenine analyses of the samples.

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Section: Methodsmentioning

confidence: 99%

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis^*

Stein¹,

Lee²,

Anderson³

et al. 1963

Biochemistry

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“…The pH was adjusted to 6.7 with 6 N KOH and the suspension was centrifuged in the cold. The supernate was passed through a column of dowex 1-formate (34), and the column was washed with water. The nucleotides were eluted with 4 N formic acid containing 1 ~ ammonium formate.…”

Section: Methodsmentioning

confidence: 99%

Protein Synthesis in Isolated Cell Nuclei

Allfrey¹,

Mirsky²,

Osawa

1955

Nature

163

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The present communication deals with the ability of isolated nuclei to incorporate isotopically labelled amino acids into their proteins. It will be shown that the deoxyribonucleic acid of the nucleus plays a role in this incorporation; that protein synthesis virtually ceases when the DNA is removed from the nucleus, and that the uptake of amino acids resumes when the DNA is restored.This report also deals with the conditions necessary for amino acid incorporation, with the effects of various inhibitors, and with the role of nucleic acid components in this aspect of protein synthesis. Preliminary accounts of some of these experiments have been published elsewhere (1, 2). Sore, Properties of Isolated Thymus Nuclei.--Most of the experiments tobe described were performed on nuclei isolated from calf thymus tissue in 0.25 M sucrose solution containing a small amount of calcium chloride. The procedure is rapid and simple, and it provides nuclei of high purity in good yield.Since some form of standardization is essential for all work on isolated cell components, thymus nuclei isolated in sucrose have been compared with those isolated in non-aqueous media (3,4). The purpose of this comparison is to test whether the aqueous isolation procedure extracts water-soluble nuclear components, or whether it leads to excessive contamination with the watersoluble proteins of the cytoplasm. Many such comparisons have shown that thymus "sucrose" nuclei are the equivalent of the standard "non-aqueous" nuclei in many respects. Their DNA content is the same (2.5 per cent DNA-P), as is their over-all protein composition and enzymic constitution (4).When preparations of thymus nuclei isolated in sucrose solution are examined under the light microscope, either stained or unstained, they seem to be a beautiful preparation. One observes great numbers of free nuclei, a few whole cells, and occasional red cells. Staining reveals occasional strands of cytoplasm attached to some of the nuclei. The over-aU extent of this cytoplasmic contamination can be estimated chemically [e.g. by nucleic acid analyses] and the conclusion was reached that these nuclei are better than 90 per cent pure.

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“…Anion-exchange chromatography has been used to separate these strongly acidic compounds. As the earlier work had shown that the 'fast-acid' fraction contained nucleotides, chromatographic methods were similar to those developed for nucleotides (Hurlbert, Schmitz, Brumm & Potter, 1954).…”

Section: Analytical Techniquesmentioning

confidence: 99%

Acidic peptides of the lens. 5. S-Sulphoglutathione

Waley

1959

Biochemical Journal

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Nucleotide Metabolism

Cited by 941 publications

References 18 publications

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis^*

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis^*

Protein Synthesis in Isolated Cell Nuclei

Acidic peptides of the lens. 5. S-Sulphoglutathione

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Nucleotide Metabolism

Cited by 941 publications

References 18 publications

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis*

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis*

Protein Synthesis in Isolated Cell Nuclei

Acidic peptides of the lens. 5. S-Sulphoglutathione

Contact Info

Product

Resources

About

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis^*

The Thionicotinamide Analogs of DPN and TPN. I. Preparation and Analysis^*