Nucleotide Excision Repair Pathway in Mycobacteria

Thakur, Manoj; Muniyappa, K.

doi:10.1007/978-981-32-9413-4_16

Cited by 3 publications

(3 citation statements)

References 175 publications

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“…With the biochemical characterization of MtUvrC (this study), the three core components of the M. tuberculosis NER pathway have been characterized in vitro [35–38,63]. Importantly, the results of this study reveal mechanistic insights into damage‐specific DNA incision function of UvrC, which is independent of UvrA and UvrB subunits.…”

Section: Discussionmentioning

confidence: 99%

The intrinsic ATPase activity of Mycobacterium tuberculosis UvrC is crucial for its damage‐specific DNA incision function

2020

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The nucleotide excision repair pathway is essential for the removal of bulky DNA adducts generated by exogenous chemicals, UV radiation and by‐products of cellular metabolism. In bacteria, the UvrAB complex recognizes and binds to the DNA adducts, and UvrC catalyses the dual incision of the damaged strand. Here, we reveal that Mycobacterium tuberculosis UvrC possesses an intrinsic ATPase activity, which is essential for the incision of the damaged DNA strand.

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Section: Discussionmentioning

confidence: 99%

The intrinsic ATPase activity of Mycobacterium tuberculosis UvrC is crucial for its damage‐specific DNA incision function

2020

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“…Examining HR using such diverse approaches and methodologies can assist in the discovery of significantly important genes that govern virulence and immune evasion in certain clinical cases of bacterial infections. Ultimately, targeting or studying HR in the genome is consistently regarded as a promising avenue for tailoring drug design strategies specieswise, , and it represents one of several approaches to combat the enduring issue of multidrug resistance in bacteria. − …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Ultimately, targeting or studying HR in the genome is consistently regarded as a promising avenue for tailoring drug design strategies specieswise, 234 , 243 and it represents one of several approaches to combat the enduring issue of multidrug resistance in bacteria. 265 − 267 …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Prokaryotic DNA Crossroads: Holliday Junction Formation and Resolution

Nautiyal,

Thakur

2024

ACS Omega

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Cells are continually exposed to a multitude of internal and external stressors, which give rise to various types of DNA damage. To protect the integrity of their genetic material, cells are equipped with a repertoire of repair proteins that engage in various repair mechanisms, facilitated by intricate networks of protein−protein and protein−DNA interactions. Among these networks is the homologous recombination (HR) system, a molecular repair mechanism conserved in all three domains of life. On one hand, HR ensures high-fidelity, template-dependent DNA repair, while on the other hand, it results in the generation of combinatorial genetic variations through allelic exchange. Despite substantial progress in understanding this pathway in bacteria, yeast, and humans, several critical questions remain unanswered, including the molecular processes leading to the exchange of DNA segments, the coordination of protein binding, conformational switching during branch migration, and the resolution of Holliday Junctions (HJs). This Review delves into our current understanding of the HR pathway in bacteria, shedding light on the roles played by various proteins or their complexes at different stages of HR. In the first part of this Review, we provide a brief overview of the end resection processes and the strand-exchange reaction, offering a concise depiction of the mechanisms that culminate in the formation of HJs. In the latter half, we expound upon the alternative methods of branch migration and HJ resolution more comprehensively and holistically, considering the historical research timelines. Finally, when we consolidate our knowledge about HR within the broader context of genome replication and the emergence of resistant species, it becomes evident that the HR pathway is indispensable for the survival of bacteria in diverse ecological niches.

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