Nucleotide excision repair. Methods of determination the efficiency functioning

Abstract

The nucleotide excision repair (NER) system is responsible for removing a wide range of bulky damages from DNA, making a significant contribution to maintaining the stability of the genome. The efficiency with which the proteins of the NER system recognize and remove bulky damages depends on many factors and has important clinical and diagnostic significance. The review examines the current understanding of the molecular basis of the functioning of the NER system in eukaryotic cells, as well as analyzes the methods and approaches that are used to study the efficiency functioning of this DNA repair system both in vitro and ex vivo.