Nucleotide diversity of Cyt b gene in drosophila subobscura Collin

Abstract

Mitochondrial DNA variability of Drosophila subobscura Collin from Southeastern Serbia was studied with respect to Restriction Site Analysis (RSA) of complete mitochondrial genome and the nucleotide sequence of Cytochrome b (Cyt b) gene. The aim was to shed more light on the evolutionary forces that shape mtDNA variation of this species. Samples were collected from two sites in the foothills of the Balkan Mountains. No genetic differentiation was found between groups and most of the variation was observed within them. Restriction analysis revealed two main haplotypes and several rare ones. The sequencing of Cyt b gene showed larger number of haplotypes, among which, one is being the most common. The majority of singletons differed from the most frequent haplotype by one nucleotide change. Although some of the observed nucleotide differences may affect their host?s fitness, the observed pattern of variation is consistent with the seasonal fluctuations in population size. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173012]

Phylogeny and diversity of lamiaceae based on rps14 gene in Pakistan

“…The present study is in accordance with the previously published articles on family Lamiaceae taxa (ZAHRA et al, 2016;(CURTO et al, 2012). Some other studied on family Lamiaceae have been done utilizing the genetic diversity through short sequencing for delimitation of the taxa in the family through using DNA barcoding (ERIĆ et al, 2019;SEVINDIK, 2019). These selected species we studied in the present work showed that they are paraphyletic which have been studied in the previous work on Lamiaceae (SCHEEN et al, 2009).…”

“…DNA sequences have become a major source of collecting information. Thus, researchers have examined the idea that an easy and rapid way of species identification and defining species boundaries is possible with the use of short DNA sequence (ERIĆ et al, 2019;SEVINDIK, 2019), which possess a standardized position in the genome, called as DNA barcode (HEBERT et al, 2004;FRÉZAL and LEBLOIS, 2008). DNA barcoding is a new approach designed to provide rapid, accurate and automatable species identification by using short standardized gene regions as internal species tags (AKINRO et al, 2019;GRITSENKO et al, 2019;PATI et al, 2019).…”

Negative frequency dependent selection contributes to the maintenance of a global polymorphism in mitochondrial DNA

“…Although SNPs do occur within each of the two haplotype groups, such SNPs are rare (e.g. [59]) and are not consistently polymorphic.…”

Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe

“…Our results show that D. obscura possesses a high level of mtDNA variation both within and between populations. The nucleotide diversity within populations observed for the D. obscura Cyt b gene (0.00146-0.00387) is generally higher compared to other Drosophila species: D. melanogaster (0.0009), D. simulans (0.0003), D. yakuba (0.0014) (Ballard & Kreitman, 1994) and D. subobscura (0.00087) (Erić et al, 2019). Sequences form a complex haplotype network with several star-shaped subnetworks, with a geographical structuring of genetic variation across Europe, mostly across the East-West axis.…”

“…A PCR assay using 16S rDNA Wolbachia-specifi c primers (O'Neill et al, 1992) was used with a slight modifi cation to the PCR conditions (García-Martínez et al, 1998). Drosophila tristis with known infection status served as a positive control (Erić et al, 2019). To exclude the possible presence of other maternally-transmitted bacteria (Hurst & Jiggins, 2005) we conducted microbiome sequencing.…”