Nucleotide and deduced amino acid sequence of a<i>Mycobacterium leprae</i>12K protein

Hartskeerl, Rudy A.; Stabel, L F; Hermans, C. J.; Klatser, P. R.; Thole, Jelle

doi:10.1093/nar/18.5.1294

Cited by 4 publications

(2 citation statements)

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“…Comparison of nucleotide and deduced amino acid sequences with known sequences was used to establish homologies and relationships with a number of well-characterized mycobacterial antigens. The DNA sequences of the clones selected with contacts' sera were compared with the sequences of the genes encoding the following proteins: 70K (14), 65K (26), 36K (PRA; 37), 28K (9), 18K (6), 12K (16), and manganese superoxide dismutase…”

Section: Resultsmentioning

confidence: 99%

Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients

et al. 1990

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A Mycobacterium leprae expression library was constructed in the vectors EX1, pEX2, and pEX3 and screened with a pool of 19 well-absorbed sera from household contacts of leprosy patients. Twelve selected recombinants that were further characterized differed clearly from recombinants selected with murine monoclonal antibodies. Whereas the monoclonal antibodies recognized mainly six recombinant antigens, the human sera from contacts reacted with a range of different recombinant antigens. None of the contact recombinant antigens was identical or related to well-characterized antigens from M. leprae or other mycobacteria selected with monoclonal antibodies, including proteins of the heat shock families. Two groups of recombinant antigens could be distinguished: one that was recognized by all sera used in the pool and one that was recognized by only a limited number of sera. These antigens, selected with sera from household contacts of previously untreated lepromatous leprosy patients, may be relevant to the immune responses during the early phase of infection with M. leprae.

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Section: Resultsmentioning

confidence: 99%

Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients

et al. 1990

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“…A reação PCR passou a ser amplamente utilizada desde que Kogan e cols 10 , em 1987, introduziram o uso de urna enzima termoestável, a Taq DNA polimerase, identificada em um microorganismo termofflico (Thermus aquaticus) 4 capaz de crescer em temperatura de 70 à 75º C. A resistência dessa enzima a temperaturas elevadas, associada a uma alta fidelidade de cópia e à sua atividade de sintese mesmo em temperaturas inferiores à temperaturede polimerização, permitem a sua edição a cada ciclo.…”

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Identificação específica do M. leprae pela técnica da Reação em Cadeia da Polimerase(PCR)

HACKEL

1990

Hansen. Int.

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A autora descreve a metodologia baseada na reação em cadeia da polimerase (PCR) para identificação especifica do DNA de Mycobacterium leprae e apresenta uma revisão das estratégias relacionadas à escolha dos oligonucleotfdios iniciadores de síntese do DNA ("primers") e dos critérios de especificidade e sensibilidade necessários à utilização desse método em estudos clínicos e experimentais

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New nucleotide sequence data on the EMBL File Server

1990

Nucl Acids Res

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Nucleotide and deduced amino acid sequence of aMycobacterium leprae12K protein

Cited by 4 publications

References 3 publications

Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients

Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients

Identificação específica do M. leprae pela técnica da Reação em Cadeia da Polimerase(PCR)

New nucleotide sequence data on the EMBL File Server

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