Nucleotide and amino acid sequence of a Cucurbita phytochrome cDNA clone: identification of conserved features by comparison with Avenu phytochrome

Sharrock, Robert A.; Lissemore, James L.; Quail, Peter H.

doi:10.1016/0378-1119(86)90072-7

Cited by 83 publications

(56 citation statements)

References 24 publications

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“…Comparison of phytochrome sequences from distantly related plant species has shown previously that the highest conservation of amino acid and nucleic acid sequence occurs in the amino-terminal half of the receptor, around the chromophore attachment site (Sharrock et al 1986). Therefore, a 0.9-kb single-stranded DNA (ssDNA) probe was prepared from the A. thaliana genomic clone covering this region of the phytochrome-coding sequence (0.9-kb probe; Fig.…”

Section: Resultsmentioning

confidence: 97%

“…The amino acid sequences of the three A. thaliana phytochromes and the published sequences of phytochromes from oat , zucchini (Sharrock et al 1986), pea (Sato 1988), rice (Kay et al 1989a), and com (Christensen and Quail 1989), have …”

Section: Phylogeny Of Phytochrome Genesmentioning

confidence: 99%

“…The cysteine residue (361) that corresponds to the chromophore attachment site identified in purified oat phytochrome is indicated by an asterisk. (Kay et al 1989a), corn (Christensen and Quail 1989), zucchini (Sharrock et al 1986), and pea (Sato 1988). …”

Section: Figure 2 [A]mentioning

confidence: 99%

“…This species is a dimer of 124-kD subunits (Jones and Quail 1986), each of which contains a covalently attached linear tetrapyrrole chromophore (Rudiger and Scheer 1983). The complete amino acid sequences of the abundant, etiolated-tissue phytochrome from oat , zucchini (Sharrock et al 1986), pea (Sato 1988), rice (Kay et al 1989a), and com (Christensen and Quail 1989), have been derived from the corresponding nucleic acid sequences. The molecular mechanism of action of phytochrome is not known.…”

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confidence: 99%

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Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family.

Sharrock¹,

Quail²

1989

Genes Dev.

795

678

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Phytochrome is a plant regulatory photoreceptor that mediates red light effects on a wide variety of physiological and molecular responses. DNA blot analysis indicates that the Arabidopsis thaliana genome contains four to five phytochrome-related gene sequences. We have isolated and sequenced cDNA clones corresponding to three of these genes and have deduced the amino acid sequence of the full-length polypeptide encoded in each case. One of these proteins ipbyA) shows 65-80% amino acid sequence identity with the major, etiolated-tissue phytochrome apoproteins described previously in other plant species. The other two polypeptides {pbyB and pbyC) are unique in that they have low sequence identity (-50%) with each other, with pbyA, and with all previously described phytochromes. The pbyA, pbyB, and pbyC proteins are of similar molecular mass, have related hydropathic profiles, and contain a conserved chromophore attachment region. However, the sequence comparison data indicate that the three pby genes diverged early in plant evolution, well before the divergence of the two major groups of angiosperms, the monocots and dicots. The steady-state level of the pbyA transcript is high in dark-grown A. tbaliana seedlings and is down-regulated by light. In contrast, the pbyB and pbyC transcripts are present at lower levels and are not strongly light-regulated. These findings indicate that the red/far red light-responsive phytochrome photoreceptor system in A. tbaliana, and perhaps in all higher plants, consists of a family of chromoproteins that are heterogeneous in structure and regulation.

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Section: Resultsmentioning

confidence: 97%

Section: Phylogeny Of Phytochrome Genesmentioning

confidence: 99%

Section: Figure 2 [A]mentioning

confidence: 99%

mentioning

confidence: 99%

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Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family.

Sharrock¹,

Quail²

1989

Genes Dev.

795

678

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“…The dimerization site has been tentatively located within a region 42 kD from the C-terminus (15,32). Even thought this region exhibits the least overall amino acid sequence identity among phytochromes from several dicots and monocots (29), the contact sites responsible for dimerization must have been sufficiently conserved through evolution to allow heterodimer formation. Although we do observe heterodimers, we expect that a majority of oat phytochrome will exist as homodimers based on the overabundance of oat phytochrome relative to tobacco phytochrome.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Tobacco Expressing Functional Oat Phytochrome

Cherry

Hershey²,

Vierstra³

1991

Plant Physiol.

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Constitutive expression of a chimeric oat phytochrome gene in tobacco (Nicotiana tabacum) results in the accumulation of a functional 124-kilodalton photoreceptor that markedly alters the phenotype of light-grown tobacco (Keller et a!. [1989] EMBO J 8: 1005EMBO J 8: -1012. Here, we provide a detailed phenotypic and biochemical characterization of homozygous tobacco expressing high levels of oat phytochrome. Phenotypic changes include a substantial inhibition of stem elongation, decreased apical dominance, increased leaf chlorophyll content, and delayed leaf senescence. Oat phytochrome synthesized in tobacco is indistinguishable from that present in etiolated oats, having photoreversible difference spectrum maxima at 665 and 730 nanometers, exhibiting negligible dark reversion of phytochrome-far redabsorbing form (Pfr) to phytochrome-red-absorbing form (Pr), and existing as a dimer with an apparent size of approximately 300 kilodaltons. Heterodimers between the oat and tobacco chromoproteins were detected. Endogenous tobacco phytochrome and transgenically expressed oat phytochrome are rapidly degraded in vivo upon photoconversion of Pr to Pfr. Breakdown of both oat and tobacco Pfr is associated with the accumulation of ubiquitin-phytochrome conjugates, suggesting that degradation occurs via the ubiquitin-dependent proteolytic pathway. This result indicates that the factors responsible for selective recognition of Pfr by the ubiquitin pathway are conserved between monocot and dicot phytochromes. More broadly, it demonstrates that the domain(s) within a plant protein responsible for its selective breakdown can be recognized by the degradation machinery of heterologous species.

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Phytochromes – Biotechnological Prospects

Reid

Jones

Smith

2004

Handbook of Plant Biotechnology

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At all stages of the life‐cycle plant growth and development processes are exceptionally responsive to signals in the light environment. Light signals can, and often do, determine when a seed germinates, when a plant flowers, the direction in which its stems grow, the balance between stem, leaf and root growth, and the interactions between a plant and it neighbours. All of these processes can be crucial to crop plant performance. More than 50 years of fundamental research has been devoted to discovering the mechanisms through which plants detect and respond to the natural light environment, and this research is now reaching the stage where the finding can be exploited in ways that will enhance crop productivity. This chapter outlines the work that has been done on phytochrome genes and their transgenic expression with a view to identifying promising approaches for the future.

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Nucleotide and amino acid sequence of a Cucurbita phytochrome cDNA clone: identification of conserved features by comparison with Avenu phytochrome

Cited by 83 publications

References 24 publications

Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family.

Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family.

Characterization of Tobacco Expressing Functional Oat Phytochrome

Phytochromes – Biotechnological Prospects

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