Nucleotide analogues to investigate RNA structure and function

Click Chemistry for Rapid Labeling and Ligation of RNA

“…[14] The ability to ligate RNA rapidly to incorporate synthetic modifications could be useful in RNA functional and structural biology for the site-specific incorporation of modified residues and atoms. [48,49] As all RNA and molecules used in this report can be obtained from commercial sources (including 5'-N 3 G), we envision this report on click labeling and ligation can be readily used to label and conjugate molecules rapidly to synthetic as well as natural RNA for biological studies. [50] The enzymatic incorporation of click-chemistry-compatible tags into RNAsynthetic, in-vitro-transcribed or cellular in origin-allows for click labeling or conjugation to a host of molecules for the detection, manipulation, analysis, or delivery of the RNA.…”

Mapping of RNA–protein interactions

“…Plenty of nucleoside analogues are available for chemical RNA modifications and it is possible to make a modification at a specified location of RNA to study its structure and function in biological systems. A number of triphosphate analogues are available and act as the substrate for RNA polymerase, leading to RNA with modifications [85]. Chemical substitutions to the base or sugar can be incorporated during in vitro transcription and on ␣-phosphorothioate substitution as the chemical tag.…”

5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 Rna Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAs

“…Modified nucleic acid systems [1–3] have facilitated mechanistic studies, [4] structural studies, [5,6] ribozyme selections, [7] in vivo siRNA delivery, [8] photochemical activity modulation, [9] and the generation of new sensors [10] and materials. [11,12] RNA-based conjugates can be prepared via phospho- ramidite chemistries or enzymatic methods, [13] where enzymatic methods allow for the preparation of longer sequences.…”