Nucleostemin, a coordinator of self-renewal, is expressed in rat marrow stromal cells and turns off after induction of neural differentiation

Myocardial Induction of Nucleostemin in Response to Postnatal Growth and Pathological Challenge

“…However, in addition to observing associations between nucleostemin and c-kit ϩ cells, we noted profound increases in nucleostemin activation in neonatal and pathologically challenged myocardium within cardiomyocytes, prompting additional studies to understand the role of nucleostemin signaling in response to myocardial injury and survival signaling. Since the discovery of nucleostemin 5 years ago 17 subsequent literature has focused predominantly on aspects of cancer, 18 -20,42,43 stem cells, 21,22,44,45 and developmental biology. 23 Previous studies of nucleostemin establish the connection between nucleostemin and proliferative populations, either in the form of stem cells 21,46 or cancer.…”

“…23 Previous studies of nucleostemin establish the connection between nucleostemin and proliferative populations, either in the form of stem cells 21,46 or cancer. 20 In this context, nucleostemin appears to be a consistent marker for maintenance of a proliferative state, because expression is rapidly lost on commitment to a differentiated postmitotic phenotype 22 and depletion of nucleostemin leads to cell cycle arrest. 47 Conversely, nucleostemin expression is rapidly induced in response to regenerative growth in the newt 24 and is required for embryonic development because nucleostemin-null mice die in blastocyst stage approximately 4 days after fertilization.…”

“…[13][14][15][16] Nucleostemin is found at high levels in various stem cells and human cancers, 17 where it has been associated with maintenance of proliferation. [17][18][19][20] Expression of nucleostemin drops precipitously during differentiation 21,22 and genetic deletion of nucleostemin results in embryonic lethality at approximately day 4 postcoitum with blastocysts comprised of cells that fail to enter S phase. 23 Similar arrest in G 0 /G 1 phase of cell cycle was observed in HeLa cells if nucleostemin was eliminated by RNA interference.…”

Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview

“…Jo et al, [76] have shown that pluripotent stem cell markers, such as Oct-4, SSEA-3, SSEA-4, Tra-1-60, and Tra-1-81, are expressed, as both mRNA and protein, by MSCs from the whole cord and their expression levels are maintained up to the 9 th passage. Furthermore, Qiao et al, [75] have detected nucleostemin, a p52 binding protein localized in nucleoli of stem cells [82], and BMI-1 involved in self-renewal [83], whereas La Rocca et al, [77] have demonstrated, for the first time, the presence of both isoforms of Oct-4 (A and B). The same Authors have extensively studied this cell population, verifying the expression of several markers related to the tumor cells, the immune recognition of MSCs by the immune system, neuroectodermal and endodermal differentiation.…”

Induction-dependent neural marker expression and electrophysiological characteristics of bone marrow mesenchymal stem cells that naturally express high levels of nestin

“…Neuronal differentiation was induced as previously described [24]. Briefly, pre-induction was performed by discarding medium and adding new MEM/F12 medium containing 20% FBS and 10 ng/mL bFGF (Pepro Tech, USA) for 24 h. On the next day, medium was removed, and neural induction medium (NIM) was added to the culture each day by discarding half of the medium and replacing with new NIM.…”