Nucleosome assembly protein 1-like 5 alleviates Alzheimer’s disease-like pathological characteristics in a cell model

Abstract

Alzheimer’s disease (AD) remains one of the most common dementias of neurodegenerative disease-related diseases. Nucleosome assembly protein 1-like 5 (NAP1L5) belongs to the NAP1L protein family, which acts as a histone chaperone. However, the function and mechanism of NAP1L5 in AD are still unclear. Bioinformatics analysis, RT-qPCR, and Western blotting results showed that NAP1L5 was downregulated in the brain tissues of AD patients and a mouse cell model of AD. NAP1L5 overexpression alleviated (Amyloid-β precursor protein) APP metabolism and Tau phosphorylation. We further demonstrated that NAP1L5 regulated the AD-like pathological characteristics through the GSK3B/Wnt/β-Catenin signaling pathway. Moreover, we showed that the Wnt/β-Catenin signaling pathway, regulated by NAP1L5, was mediated by AQP1-mediated mechanism in N2a-APP695sw cell. In sum, these results suggested that NAP1L5 overexpression has neuroprotective effects and might act as potential biomarker and target for the diagnosis and treatment of AD.

Integrative analysis from multi‐center studies identifies a weighted gene co‐expression network analysis‐based Tregs signature in ovarian cancer

“…38 NAP1L5 is a nucleosome assembly protein that is involved in regulating chromatin structure and gene expression. 25 The study by Zhao and colleagues examined the function of NAP1L5 in hepatocellular carcinoma (HCC), and low expression of NAP1L5 was found in HCC, and its downregulation was associated with shorter survival and disease-free survival. 39 In addition, the study found that NAP1L5 inhibits the PI3K/AKT/mTOR signaling pathway in HCC by regulating MYH9, suggesting that NAP1L5 may be a potential target.…”

“…Another study demonstrated that stable expression of HOXD8 inhibits cell proliferation, anchorage‐independent growth, and invasion, and can induce apoptotic events in CRC cells by upregulating executioner caspases 6 and 7 and cleaved PARP 38 . NAP1L5 is a nucleosome assembly protein that is involved in regulating chromatin structure and gene expression 25 . The study by Zhao and colleagues examined the function of NAP1L5 in hepatocellular carcinoma (HCC), and low expression of NAP1L5 was found in HCC, and its downregulation was associated with shorter survival and disease‐free survival 39 .…”

“…The mRNA expression levels were normalized to GAPDH mRNA level and calculated using the 2 −ΔΔCt method. The primer sequences were available in the previous references, including CD24, 21 FHL2, 22 GPM6A, 23 HOXD8, 24 NAP1L5, 25 REN, 26 and TOX3 27 …”