Nucleoside transport across the plasma membrane mediated by equilibrative nucleoside transporter 3 influences metabolism of <i>Arabidopsis</i> seedlings

Cornelius, Stefanie; Traub, Michaela; Bernard, C.; Salzig, Christian; Lang, Patrick; Möhlmann, Torsten

doi:10.1111/j.1438-8677.2012.00562.x

Cited by 21 publications

(21 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Five transport amino nitrogen compounds including two aquaglyceroporins (NIP1-2; nodulin26 – [ 75 ]; Additional file 14 ). Two nucleoside proton symporters, adenine/guanine permease AZG1 [ 76 ] and equilibrative nucleoside transporter 3 [ 77 ], likely function to retrieve apoplasmic nucleosides released from degradation of endosperm and inner cell layers of seed coats crushed by expanding cotyledons. Collectively, expression of these transporters reflects a demand for nitrogenous precursors to support protein biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Differential transcriptional networks associated with key phases of ingrowth wall construction in trans-differentiating epidermal transfer cells of Vicia faba cotyledons

et al. 2015

View full text Add to dashboard Cite

BackgroundTransfer cells are characterized by intricate ingrowth walls, comprising an uniform wall upon which wall ingrowths are deposited. The ingrowth wall forms a scaffold to support an amplified plasma membrane surface area enriched in membrane transporters that collectively confers transfer cells with an enhanced capacity for membrane transport at bottlenecks for apo-/symplasmic exchange of nutrients. However, the underlying molecular mechanisms regulating polarized construction of the ingrowth wall and membrane transporter profile are poorly understood.ResultsAn RNAseq study of an inducible epidermal transfer cell system in cultured Vicia faba cotyledons identified transfer cell specific transcriptomes associated with uniform wall and wall ingrowth deposition. All functional groups of genes examined were expressed before and following transition to a transfer cell fate. What changed were the isoform profiles of expressed genes within functional groups. Genes encoding ethylene and Ca2+ signal generation and transduction pathways were enriched during uniform wall construction. Auxin-and reactive oxygen species-related genes dominated during wall ingrowth formation and ABA genes were evenly expressed across ingrowth wall construction. Expression of genes encoding kinesins, formins and villins was consistent with reorganization of cytoskeletal components. Uniform wall and wall ingrowth specific expression of exocyst complex components and SNAREs suggested specific patterns of exocytosis while dynamin mediated endocytotic activity was consistent with establishing wall ingrowth loci. Key regulatory genes of biosynthetic pathways for sphingolipids and sterols were expressed across ingrowth wall construction. Transfer cell specific expression of cellulose synthases was absent. Rather xyloglucan, xylan and pectin biosynthetic genes were selectively expressed during uniform wall construction. More striking was expression of genes encoding enzymes for re-modelling/degradation of cellulose, xyloglucans, pectins and callose. Extensins dominated the cohort of expressed wall structural proteins and particularly so across wall ingrowth development. Ion transporters were selectively expressed throughout ingrowth wall development along with organic nitrogen transporters and a large group of ABC transporters. Sugar transporters were less represented.ConclusionsPathways regulating signalling and intracellular organization were fine tuned whilst cell wall construction and membrane transporter profiles were altered substantially upon transiting to a transfer cell fate. Each phase of ingrowth wall construction was linked with unique cohorts of expressed genes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0486-5) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential transcriptional networks associated with key phases of ingrowth wall construction in trans-differentiating epidermal transfer cells of Vicia faba cotyledons

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Research over the last years identified nucleosides as valuable metabolic intermediates involved in different aspects of plant metabolism (Jung et al, 2009 , 2011 ; Cornelius et al, 2012 ; Girke et al, 2014 ). Here, we describe the generation of a mutant lacking the main nucleoside import activity as well as extracellular nucleoside hydrolase activity.…”

Section: Discussionmentioning

confidence: 99%

Apoplastic Nucleoside Accumulation in Arabidopsis Leads to Reduced Photosynthetic Performance and Increased Susceptibility Against Botrytis cinerea

et al. 2015

View full text Add to dashboard Cite

Interactions between plant and pathogen often occur in the extracellular space and especially nucleotides like ATP and NAD have been identified as key players in this scenario. Arabidopsis mutants accumulating nucleosides in the extracellular space were generated and studied with respect to susceptibility against Botrytis cinerea infection and general plant fitness determined as photosynthetic performance. The mutants used are deficient in the main nucleoside uptake system ENT3 and the extracellular nucleoside hydrolase NSH3. When grown on soil but not in hydroponic culture, these plants markedly accumulate adenosine and uridine in leaves. This nucleoside accumulation was accompanied by reduced photosystem II efficiency and altered expression of photosynthesis related genes. Moreover, a higher susceptibility toward Botrytis cinerea infection and a reduced induction of pathogen related genes PR1 and WRKY33 was observed. All these effects did not occur in hydroponically grown plants substantiating a contribution of extracellular nucleosides to these effects. Whether reduced general plant fitness, altered pathogen response capability or more direct interactions with the pathogen are responsible for these observations is discussed.

show abstract

“…7, no. 47; Traub et al, 2007;Cornelius et al, 2012) or further hydrolyzed by the apoplastic purine-specific NSH3 (Fig. 7, no.…”

Section: Pyrimidine Nucleotide Degradationmentioning

confidence: 99%

Nucleotide Metabolism in Plants

2019

View full text Add to dashboard Cite

Nucleoside transport across the plasma membrane mediated by equilibrative nucleoside transporter 3 influences metabolism of Arabidopsis seedlings

Cited by 21 publications

References 44 publications

Differential transcriptional networks associated with key phases of ingrowth wall construction in trans-differentiating epidermal transfer cells of Vicia faba cotyledons

Differential transcriptional networks associated with key phases of ingrowth wall construction in trans-differentiating epidermal transfer cells of Vicia faba cotyledons

Apoplastic Nucleoside Accumulation in Arabidopsis Leads to Reduced Photosynthetic Performance and Increased Susceptibility Against Botrytis cinerea

Nucleotide Metabolism in Plants

Contact Info

Product

Resources

About