Nucleoside-Nucleotide Mixture Increases Bone Marrow Cell Number and Small Intestinal RNA Content in Protein-Deficient Mice After an Acute Bacterial Infection

Yamauchi, Keiko; Adjei, Andrew A.; Ameho, Clement K.; Sato, Seiji; Okamoto, Keiichi; Kakinohana, Shige; Yamamoto, Shigeru

doi:10.1016/s0899-9007(97)00469-3

Cited by 12 publications

(5 citation statements)

References 20 publications

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“…De novo synthesis of nucleotides is also limited or absent in hematopoietic bone marrow cells 117 . Protein‐deficient mice subjected to acute S aureus infection displayed increased proliferation in bone marrow cells and bone marrow DNA content after intraperitoneal (IP) administration of a nucleoside‐nucleotide mixture.…”

Section: Representative Animal Studies With Nucleotidesmentioning

confidence: 99%

The Role of Nucleotides in the Immune and Gastrointestinal Systems

2012

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Nucleotides are low molecular weight biological molecules key to biochemical processes. Sources include de novo synthesis, recovery via salvage mechanisms, and dietary intakes. Although endogenous production serves as the main nucleotide source, evidence suggests that exogenous sources are essential to immune competence, intestinal development, and recovery. Dietary nucleotides serve a marked role in rapidly proliferating cells where they are necessary for optimal function. Accordingly, dietary nucleotides are deemed conditionally essential in the presence of various physiological stresses, including growth and development, recovery from injury, infection, and certain disease states. Clinical studies that evaluated nutrition formulations of nucleotides in combination with other specific nutrient substances demonstrated improved clinical outcomes in patients characterized as critically ill, injured, immune suppressed, or with chronic gastrointestinal conditions. However, conclusions regarding specific benefits of nucleotides are limited. Scientific substantiation of nucleotide supplementation in infant formula has been reported to improve the maturation and development of the intestinal tract as well as immune function. All medical nutrition products except for one immune-modulating formulation are devoid of nucleotides. In an effort to build on this, the current review presents the data to support potential clinical applications for nucleotides in enteral nutrition that may contribute to improved outcomes in physiologically stressed patients.

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Section: Representative Animal Studies With Nucleotidesmentioning

confidence: 99%

The Role of Nucleotides in the Immune and Gastrointestinal Systems

2012

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“…Besides, nucleotides act as a fundamental component in carbohydrate, protein, fat and nucleic acid metabolism ( Carver & Walker, 1995 ; Cosgrove, 1998 ). Therefore, they play a critical role in Protein synthesis ( Yamauchi et al., 1998 ), Cell mitosis ( Carver & Walker, 1995 ), Lipid metabolism ( Gil, Corral, Martinez & Molina, 1986 ), hematopoiesis ( Yamamoto, Wang, Adjei & Ameho, 1997 ), immunity ( Hung, 2015 ) and gut health ( Martinez-Puig et al., 2007 ; Sauer et al., 2012a ).…”

Section: Introductionmentioning

confidence: 99%

The impact of exogenous dietary nucleotides in ameliorating Clostridium perfringens infection and improving intestinal barriers gene expression in broiler chicken

Mohammed

Hady

Kamel

et al. 2020

Veterinary and Animal Science

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Highlights Nucleotides are an integral part of the genetic information in each cell. Nucleotides are naturally present in all feeds of animal and vegetable origin but lower concentrations. Dietary nucleotides improve the intestinal barrier function and intestinal histomorphology which positively reflected on the growth performance of C.perfringens infected chickens.

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“…These results revealed that nucleoside-injected groups showed a higher protein concentration than the control group in all the periods. Intraperitoneal administration of nucleoside-nucleotide mixture increased total protein concentration in mice (24). This indicates that nucleotides are essential to increase body protein turnover rate, specific protein synthesis, and enterocyte proliferation.…”

Section: Plasma Concentration Of Total Protein and Uric Acidmentioning

confidence: 87%

“…The box-plot analysis of Cdx gene expression revealed upregulation in all the three doses of in ovo injection compared to both the controls. Intraperitoneal administration of nucleoside-nucleotide mixture increased small intestinal RNA levels in mice, which indicated an increased proliferation of enterocytes compared to the control group (24). Supplementation of nucleotides provides benefits to enterocyte function during normal periods of growth and development characterized by high demand for DNA and RNA synthesis (33).…”

Section: Intestinal Morphology and Development Studiesmentioning

confidence: 99%

In ovo Administration of Nucleosides Improved the Performance, Apparent Metabolizable Energy and Gut Development in Broiler Chickens

et al. 2020

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An in ovo study on the effect of the administration of a combination of nucleosides (25, 50, and 100 mg/egg) on hatchability, growth performance, energy metabolizability, and intestinal morphology in broilers was carried out. Four hundred eighty (480) fertile eggs were divided into four groups (in four replicates each having 30 eggs). On the 18th days of incubation of the eggs, candling was carried out and the fertile eggs were selected and given one of the four in ovo administrations. Group one served as control and was injected with phosphate-buffered saline (PBS). The other groups were given in ovo administration of nucleosides (25, 50, and 100 mg/egg) at 100 μl through the yolk sac route, and chicks of respective groups were hatched out. Among the experimental groups, the hatchability was comparable; however, the hatchability was affected in the group injected with a higher level of nucleosides at 100 mg/egg. The hatched out chicks from higher doses of nucleosides (50 and 100 mg) had higher body weight (BW) (P < 0.05) than the control. Higher energy metabolizability (%) was observed in nucleoside-injected groups. Plasma protein concentration was higher in groups administered with nucleosides (50 and 100 mg). Histologically, the intestinal villi length was maximum in 100 mg-injected group followed by 50 and 25 mg. Relative expression of homeobox (Cdx) in the jejunum was significantly (P < 0.05) upregulated in all the injected groups at 3, 7, and 14 days of age. Nucleoside-administered groups had better performance, energy metabolizability, and intestinal morphology. Among the experimental groups, the administration of nucleosides at 50 mg/egg resulted in higher growth performance, plasma protein, intestinal surface, and villi development in broiler chickens.

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Nucleoside-Nucleotide Mixture Increases Bone Marrow Cell Number and Small Intestinal RNA Content in Protein-Deficient Mice After an Acute Bacterial Infection

Cited by 12 publications

References 20 publications

The Role of Nucleotides in the Immune and Gastrointestinal Systems

The Role of Nucleotides in the Immune and Gastrointestinal Systems

The impact of exogenous dietary nucleotides in ameliorating Clostridium perfringens infection and improving intestinal barriers gene expression in broiler chicken

In ovo Administration of Nucleosides Improved the Performance, Apparent Metabolizable Energy and Gut Development in Broiler Chickens

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