Nucleoside diphosphate kinase 2 confers acquired 5-fluorouracil resistance in colorectal cancer cells

Wen, Shaojia; Wang, Xun; Wang, Yamin; Shen, Jianfeng; Pu, Junyi; Liang, Hui; Chen, Chao; Liu, Linna; Dai, Peng

doi:10.1080/21691401.2018.1439835

Cited by 17 publications

(12 citation statements)

References 30 publications

(26 reference statements)

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“…Total RNA from cell lines was extracted using TRIzol reagent according to a previously described method [20]. For genes detection, the samples were amplified by PCR using One…”

Section: Rna Extraction Reverse Transcription and Qrt-pcrmentioning

confidence: 99%

MiR-483-3p regulates oxaliplatin resistance by targeting FAM171B in human colorectal cancer cells

Liang

Qiang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Oxaliplatin resistance limits the efficiency of treatment for colorectal cancer (CRC). Studies have shown that abnormal expression of microRNAs (miRNAs) were associated with tumorigenesis, cancer development and chemoresistance. The purpose of this study was to identify potential miRNAs related to oxaliplatin resistance in CRC cells. In this work, using small RNA sequencing (small RNA-Seq) and transcriptome sequencing (RNA-Seq), we found that down-regulated miR-483-3p was concurrent with up-regulated FAM171B in oxaliplatin-resistant colorectal cancer cell line HCT116/L as compared with its parental cell line HCT116. Transient transfection of miR-483-3p mimics markedly decreased the levels of FAM171B and restored oxaliplatin responsiveness of HCT116/L cells, and this alteration enhanced cell apoptosis and weakened cell migration. Whereas miR-483-3p inhibitor dramatically promoted the expression of FAM171B and enhanced oxaliplatin resistance of HCT116 cells by repressing cell apoptosis. Furthermore, knockdown of FAM171B in HCT116/L cells could also sensitize its reaction of the treatment with oxaliplatin, which was verified by the reduced cell migration. These findings demonstrate that FAM171B is a functional target of miR-483-3p in the regulation of oxaliplatin resistance in human CRC cells.

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“…Total RNA from cell lines was extracted using TRIzol reagent according to a previously described method [20]. For genes detection, the samples were amplified by PCR using One…”

Section: Rna Extraction Reverse Transcription and Qrt-pcrmentioning

confidence: 99%

MiR-483-3p regulates oxaliplatin resistance by targeting FAM171B in human colorectal cancer cells

Liang

Qiang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…The increased number of ribosomal protein L30 (RPL30) may play a central role in the CRC process and in induced hepatocellular carcinoma [32,33]. The overexpression of NME2 was significantly associated with not only clinical parameters related to tumor progression, invasion, and metastasis but also resistance to 5-FU treatment [34,35]. The low expression of USP33 indicated a high recurrence risk and poor overall prognosis in advanced CRC patients [36].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Biomarkers and Biological Pathways for Poor Clinical Outcome in Mucinous Colorectal Adenocarcinoma

Kim

Myung

Kwak

2021

Cancers

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Colorectal cancer (CRC) comprises several histological subtypes, but the influences of the histological subtypes on prognosis remains unclear. We sought to evaluate the prognosis of mucinous adenocarcinoma (MAC), compared to that of traditional adenocarcinoma (TAC). This study used the data of patients diagnosed with CRC between 2004 and 2016, as obtained from the Surveillance, Epidemiology, and End Results database. We established a predictive model for disease-specific survival using conditional survival forest, model, non-linear Cox proportional hazards, and neural multi-task logistic regression model and identified the gene signatures for predicting poor prognosis based on the arrayexpress datasets. In total, 9096 (42.1%) patients with MAC and 12,490 (58.9%) patients with TAC were included. Those with the MAC subtype were more likely to have a poorer overall survival rate compared to those with the TAC subtype in stage II CRC (p = 0.002). The eight major genes including RPS18, RPL30, NME2, USP33, GAB2, RPS3A, RPS25, and CEP57 were found in the interacting network pathway. MAC was found to have a poorer prognosis compared to TAC, especially in Stage II CRC. In addition, our findings suggest that identifying potential biomarkers and biological pathways can be useful in CRC prognosis.

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“…We compare variant genes of each patient with the genetic susceptibility of genes databases including Cancer Gene Census (CGC) database [9] and two susceptibility gene databases [10,11]. Then, to better illustrate the potential functional mechanism of the varied genes(cellular process, metabolic process, single-organism process and catalytic activity [12]), the Kyoto encyclopedia of genes and genomes (KEGG) pathway and gene ontology (GO) functional enrichment analysis were carried out using the database for annotation, visualization and integrated discovery the DAVID Bioinformatics Tool [13], which consists of an integrated biological knowledge base and analytic tools aimed at systematically extracting biological meaning from large gene/protein lists. To do this, firstly, the genes involved in the functional analysis.…”

Section: Crucial Genes Identification and Functional Analysismentioning

confidence: 99%

Analysis of polymorphisms in genes associated with the FA/BRCA pathway in three patients with multiple primary malignant neoplasms

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Cases of more than three primary cancers are very rare. This study analyzed the genetic susceptibility of gene polymorphisms in three patients with multiple primary malignant neoplasms and examined the possible pathogenesis. The clinical data and whole genome sequence of three patients (1 with 5 primary cancers, 1 with 4 primary cancers, and 1 with 3 primary cancers) were aligned with a series of databases. We found the three patients contained a total of seven types of malignant tumours (endometrial cancer, ovarian cancer, breast cancer, colon cancer, ureter cancer, bladder cancer and kidney cancer). It was found that the varied genes in Patient 1 (5 primary cancers) were BRIP1, FANCG, NBN, AXIN2, SRD5A2, and CEBPA. Patient 2 (4 primary cancers) had variations in the following genes: BMPR1A, FANCD2, MLH3, BRCA2, and FANCM. Patient 3 (3 primary cancers) had variations in the following genes: MEN1, ATM, MSH3, BRCA1, FANCL, CEBPA, and FANCA. String software was used to analyze the KEGG pathway of the variations in these three samples, which revealed that the genes are involved in the Fanconi anaemia pathway. Defects in DNA damage repair may be one of the causes of multiple primary cancers.

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Nucleoside diphosphate kinase 2 confers acquired 5-fluorouracil resistance in colorectal cancer cells

Cited by 17 publications

References 30 publications

MiR-483-3p regulates oxaliplatin resistance by targeting FAM171B in human colorectal cancer cells

MiR-483-3p regulates oxaliplatin resistance by targeting FAM171B in human colorectal cancer cells

Identification of Potential Biomarkers and Biological Pathways for Poor Clinical Outcome in Mucinous Colorectal Adenocarcinoma

Analysis of polymorphisms in genes associated with the FA/BRCA pathway in three patients with multiple primary malignant neoplasms

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