Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?

Tozetto‐Mendoza, Tânia Regina; Kanunfre, Kelly Aparecida; Vilas-Boas, Lucy Santos; Espinoza, Evelyn Patrícia Sánchez; Paião, Heuder Gustavo Oliveira; Rocha, Mussya Cisotto; A, de Paula; Oliveira, Maura Salaroli de; Zampelli, Daniella Bosco; Vieira, J.M.; Buss, Lewis; Costa, Sílvia Figueiredo; Sabino, Éster Cerdeira; Okay, Thelma Suely; Mendes‐Correa, Maria Cássia

doi:10.1016/j.jviromet.2021.114064

Cited by 17 publications

(23 citation statements)

References 27 publications

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“…The N-protein structure [ 22 ], unlike the S-protein RBD [ 19 , 20 , 23 ], does not show cross-reactivity with anti-dengue antibodies [ 24 , 25 , 26 , 27 ] and is not targeted by the current approved COVID-19 vaccines [ 28 ]. Thus, the utility of N-protein immunoassays, rather than S-protein immunoassays, should be considered to assess the exposure to SARS-CoV-2 infection and to estimate the incidence of COVID-19 disease in dengue endemic populations.…”

Section: Introductionmentioning

confidence: 99%

Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection

Lytton¹,

Yeasmin

Ghosh

et al. 2021

Pathogens

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Background: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirus nucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh. Methods: Viral RNA was evaluated in swab specimens from 115 COVID-19 patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virus E-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19 patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA. Results: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9–23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27–30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsid IgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations. Conclusions: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19 patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.

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Section: Introductionmentioning

confidence: 99%

Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection

Lytton¹,

Yeasmin

Ghosh

et al. 2021

Pathogens

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Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

“…For SARS-CoV-2, several studies had reported the use of ELISA to detect antibodies against N protein of SARS-CoV and the sensitivity and specificity of such test ranged from 90 to 94%, and, above 97%, respectively ( Table 11 ) [202] , [203] . Compared to ELISA assay that was aimed to detect antibodies like IgG against the N protein of SARS-CoV-2 [202] , [203] , the use of ELISA test that was focused to detect antibodies against S protein of SARS-CoV-2 showed wide range values of sensitivity and specificity, according to the findings reported from various studies [199] , [204] , [205] , [206] , [207] . Some studies showed that S protein-based ELISA test was less sensitive to detect IgG and IgA against SARS-CoV-2 (<70%) [206] , [207] while few other studies reported that such test was highly sensitive (at least 90%) to detect IgG, IgA, or IgM against the virus S antigen [199] , [204] , [205] , [208] .…”

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

“…Some studies showed that S protein-based ELISA test was less sensitive to detect IgG and IgA against SARS-CoV-2 (<70%) [206] , [207] while few other studies reported that such test was highly sensitive (at least 90%) to detect IgG, IgA, or IgM against the virus S antigen [199] , [204] , [205] , [208] . Therefore, N protein-based ELISA assay could be a better choice of ELISA test to predict serological response in the SARS-CoV-2 patients as the test results of this assay seem to be more consistent and vary less in different reported studies [202] , [203] as compared to the S protein-based ELISA test [199] , [204] , [206] , [207] , [208] , [209] . On the other hand, some studies had reported the use of ELISA test that was aimed to detect different antibodies against different proteins of SARS-CoV-2, and examples of these tests were tests that were focusing to detect antibodies against N and S protein of SARS-CoV-2 [196] , [204] , [209] , [210] , [211] , [212] , [213] , [214] , [215] .…”

Section: Different Diagnostic Approaches To Detect Mers-cov and Sars-cov-2 Infectionsmentioning

confidence: 99%

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Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Chong

Liew

Ong

et al. 2021

Pathology - Research and Practice

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Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are two common betacoronaviruses, which are still causing transmission among the human population worldwide. The major difference between the two coronaviruses is that MERS-CoV is now causing sporadic transmission worldwide, whereas SARS-CoV-2 is causing a pandemic outbreak globally. Currently, different guidelines and reports have highlighted several diagnostic methods and approaches which could be used to screen and confirm MERS-CoV and SARS-CoV-2 infections. These methods include clinical evaluation, laboratory diagnosis (nucleic acid-based test, protein-based test, or viral culture), and radiological diagnosis. With the presence of these different diagnostic approaches, it could cause a dilemma to the clinicians and diagnostic laboratories in selecting the best diagnostic strategies to confirm MERS-CoV and SARS-CoV-2 infections. Therefore, this review aims to provide an up-to-date comparison of the advantages and limitations of different diagnostic approaches in detecting MERS-CoV and SARS-CoV-2 infections. This review could provide insights for clinicians and scientists in detecting MERS-CoV and SARS-CoV-2 infections to help combat the transmission of these coronaviruses.

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“…Under the current circumstances, where millions of viral infections exist, it is undeniable that vaccination is of vital importance, and immunoassays have taken a particularly relevant role in identifying and monitoring patients’ immune responses over time. Therefore, several research groups have proposed various COVID-19-related immunoassays, which are mainly based on viral antigens, such as the spike protein [ 13 ], receptor-binding domain (RBD) [ 14 ], and nucleoprotein [ 15 ], and include different strategies ranging from the traditional enzyme-linked immunoassay (ELISA) to more complex microfluidic immunoassays [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Automated ELISA On-Chip for the Detection of Anti-SARS-CoV-2 Antibodies

Gonzalez‐González

Garcia-Ramirez²,

Díaz-Armas³

et al. 2021

Sensors

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The COVID-19 pandemic has been the most critical public health issue in modern history due to its highly infectious and deathly potential, and the limited access to massive, low-cost, and reliable testing has significantly worsened the crisis. The recovery and the vaccination of millions of people against COVID-19 have made serological tests highly relevant to identify the presence and levels of SARS-CoV-2 antibodies. Due to its advantages, microfluidic-based technologies represent an attractive alternative to the conventional testing methodologies used for these purposes. In this work, we described the development of an automated ELISA on-chip capable of detecting anti-SARS-CoV-2 antibodies in serum samples from COVID-19 patients and vaccinated individuals. The colorimetric reactions were analyzed with a microplate reader. No statistically significant differences were observed when comparing the results of our automated ELISA on-chip against the ones obtained from a traditional ELISA on a microplate. Moreover, we demonstrated that it is possible to carry out the analysis of the colorimetric reaction by performing basic image analysis of photos taken with a smartphone, which constitutes a useful alternative when lacking specialized equipment or a laboratory setting. Our automated ELISA on-chip has the potential to be used in a clinical setting and mitigates some of the burden caused by testing deficiencies.

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Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?

Cited by 17 publications

References 27 publications

Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection

Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Automated ELISA On-Chip for the Detection of Anti-SARS-CoV-2 Antibodies

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