Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis

Abstract

Background: Nuclear pore complexes (NPCs) act as nano-turnstiles within nuclear membranes between the cytoplasm and nucleus of mammalian cells. NPC proteins, called nucleoporins (Nups), mediate trafficking of proteins and RNA into and out of the nucleus, and are involved in a variety of mitotic processes. We previously reported that Nup62 localizes to the centrosome and mitotic spindle during mitosis, and plays a role in centrosome homeostasis. However, whether Nup58, a Nup62 subcomplex protein, also localizes to spindle poles is unknown. Result: Herein, we show that Nup58 localizes to the nuclear rim during interphase, and to mitotic spindles, centrosomes, and midbodies during mitosis. Our confocal microscopy, live-cell imaging, and stimulated emission depletion nanoscopy results also demonstrated that Nup58 localized to the centrosomes during metaphase and relocalized to midbodies during abscission. Depletion of Nup58 resulted in centrosomal abnormalities and delayed abscission. Conclusion: Nup58 localized at the centrosomes and mitotic spindle during metaphase and relocalized at midbodies during abscission. This study highlights the important role of Nup58 in mitosis.

Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis

“…In these GFP-Nup62 K432R -expressing cells, apparent spindle orientation defects were seen as the two spindle poles did not exist at the same focal plane ( Figure 5 A; arrows). To further evaluate whether the observed mitotic perturbation were really due to Nup62 knockdown but not the downregulation or aberrant localization of other Nups in the Nup62 complex ( Hartono et al, 2019 ), we examined the localization dynamics of Nup58 in Nup62-depleted cells in mitosis. Interestingly, GFP-Nup58-expressing cells depleted of Nup62 showed normal localization to NPC in prophase and to centrosome during proceeding phases ( Figure 5 A; bottom panel).…”

Parentage testing and looking for single nucleotide markers associated with antler quality in deer (Cervus elaphus)

“…Exocyst complex component 6B (EXOC6B) is associated with joint dislocation syndrome (Girisha et al, 2016). Nucleoporin 58 (NUP58) mediates molecular trafficking to and from the nucleus and can be tied to delayed abscission during mitosis (Hartono et al, 2019). Spermatogenesis associated 13 (SPATA13) can weaken the migration of type I collagen via activation of GTPase (Jean et al, 2013).…”