Nucleoporin 107 Promotes the Survival of Tumor Cells in Cervical Cancers

Abstract

<b><i>Aims:</i></b> Recent studies indicate that in addition to the construction of nuclear pore complex, nucleoporin (NUP)107 is actively involved in the pathogenesis of numerous cancer types, but the role of NUP107 in cervical carcinoma cells remains unknown. <b><i>Methods:</i></b> We examined the expression of NUP107 in 30 cases of cervical carcinoma using quantitative reverse transcription-polymerase chain reaction and immunoblots. NUP107 stably overexpressing cell line was established to examine the function of NUP107 in cell viability, TUNEL assay, wound healing assay, 5-ethynyl-2’-deoxyuridine incorporation, and oxidative stress. <b><i>Results:</i></b> NUP107 expression was significantly increased in the cervical carcinoma tissues, compared with their corresponding adjacent normal tissues. Overexpression of NUP107 conferred the cervical cancer cells with significant resistance to oxidative insult, but it had no effects on the migration and proliferation. This pro-survival function of NUP107 was associated with the elevated expression of Bcl-2, the activation of Akt signaling, and increased expression of nucleocytoplasmic transport factors. Silencing of NUP107 increased the sensitivity of cervical cancer cells to oxidative challenge, thereby inducing the apoptosis of cervical cancer cells. <b><i>Conclusion:</i></b> NUP107 is significantly increased in cervical tissues and confers the cervical cancer cells with resistance to oxidative damage. These results provide an important role for specific NUP in mediating cervical cancer.

Promoting cell proliferation, cell cycle progression, and glycolysis: Glycometabolism‐related genes act as prognostic signatures for prostate cancer

“…NUP107 was also confirmed as a key regulator of the intra‐S‐phase checkpoint response, which is induced by DNA damage 46 . The role of NUP107 in cell proliferation may vary in different cell lines 47 . Although significant proliferation and apoptosis inhibition did not occur after knockdown of NUP107 , this might be because nucleoporin has considerable functional redundancy, as was previously reported 48 .…”

Comprehensive analysis of ZFPM2-AS1 prognostic value, immune microenvironment, drug sensitivity, and co-expression network: from gastric adenocarcinoma to pan-cancers

“…For example, Shi et al demonstrated that the expression of NUP107 is significantly upregulated in cervical cancer samples and cell lines. Besides, overexpression of NUP107 promotes the sensitivity of cervical cancer cells to oxidative insults [ 43 ]. Alanee et al discovered that a single nucleotide variant in NUP107 might be predictive of chemosensitivity in ovarian cancer patients [ 44 ].…”

Weighted Gene Coexpression Network Analysis of Key Genes Controlling Cancer Stem Cell Characteristics by Mrnasi in Colon Adenocarcinoma

“…MELK expression was correlated with resistance of radiation and chemotherapy in colorectal cancer[108], as well as survival of patients with multiple cancer types [109][110][111].The clinical trials and basic experiments for cancers with MELK inhibitor are ongoing. The overexpression of NUP107 increased resistance to oxidative damage in cervical cancer [112]. Previous studies have indicated that DEPDC1B induces tumor cell proliferation, migration, invasion and metastasis through various mechanisms [113][114][115][116].…”