“…Toluene dioxygenase (TDO) catalyzes the biotransformation of benzonitrile to cis-(1S,2R)-1,2-dihydroxy-3-cyanocyclohexane-3,5-diene, [53] (2-cyanoethyl)benzene to (5S,6R)-1-(2-cyanoethyl)-5,6-dihydroxycyclohexa-1,3-diene [54] and trans-cinnamonitrile to (trans-cinnamonitrile)-cis-2,3-dihydrodiol. [55] CDO catalyzes the transformation of benzonitrile to cis-dihydroxy-cyclohexadienecarbonitrile and benzyl cyanide to 1-(cis-1,2-dihydroxy-3,5-cyclohexadienyl)-acetonitrile (main product 98%), 2-(cis-2,3-dihydroxy-4,6-cyclohexadienyl)-acetonitrile, and 1-(cis-3,4-dihydroxy-1,5-cyclohexadienyl)-acetonitrile.…”