Nucleolus-tethering system (NoTS)

Liu, Yin; Fang, Yuda

doi:10.4161/nucl.29837

Cited by 2 publications

(3 citation statements)

References 51 publications

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“…Next, in order to further study the subcellular localization of NP1 and NOS-like enzyme, and the specificity of NP1 to NOS-like enzyme, a plasmid PC131-35s-iNOS-mCherry including genes of iNOS and a red fluorescent protein named mCherry was constructed 32 33 . The length of gene of iNOS was 3438 bp, and the experimental results were consistent with it ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

Chang

Guo

et al. 2016

Sci Rep

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Nitric oxide synthase like enzyme (NOS-like enzyme), which produces nitric oxide, participates in many biological processes. However it remains unidentified and highly controversial that plants do possess a NOS-like enzyme. In this paper, a novel arginine analogue NP1 was designed and developed for the direct identification and real time tracking of NOS-like enzymes in plant by fluorescence sensing. It could bind NOS-like enzyme efficiently and enter the cell successfully. In vivo fluorescence response results directly proved that NOS-like enzymes did exist in tobacco leaf and would be stimulated by pathogen infection, which also provided a useful chemical tool for the study of the function of NOS-like enzyme in plants.

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Section: Resultsmentioning

confidence: 99%

Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

Chang

Guo

et al. 2016

Sci Rep

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“…The traditional methods for mechanism studies usually depend on immunoassays and immunocytochemical analysis. , They are comparatively expensive and time-consuming and cannot analyze the targets in a living sample directly. A fluorescence sensor-based detection method has many advantages, such as convenience, high sensitivity, nondestructiveness, and use in real time; it has been widely applied in chemical, biological, and medical areas. − With the development of fluorescent proteins and fluorescence microscopes, many techniques have been exploited on the basis of fluorescence, such as the nucleolus-tethering system (NoTS). , In this paper, on the basis of the derivation of the active fluorescence plant activators N-FBT and the construction of a new nucleolus-tethering system (New-NoTS) especially for the study of interactions between small molecules and proteins, we will study the interactions between N-FBT and key proteins in the JA and SA pathways and deduce their mechanism of action in plants.…”

Section: Introductionmentioning

confidence: 99%

“…36−41 With the development of fluorescent proteins and fluorescence microscopes, many techniques have been exploited on the basis of fluorescence, such as the nucleolus-tethering system (NoTS). 42,43 In this paper, on the basis of the derivation of the active fluorescence plant activators N-FBT and the construction of a new nucleolus-tethering system (New-NoTS) especially for the study of interactions between small molecules and proteins, we will study the interactions between N-FBT and key proteins in the JA and SA pathways and deduce their mechanism of action in plants.…”

Section: ■ Introductionmentioning

confidence: 99%

Active Fluorescence Plant Activator N-FBT: A New Tool for the Study of Defense Signaling Pathway in Plants

Chang

Mei

Guo

et al. 2021

ACS Agric. Sci. Technol.

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Traditional methods for mechanism studies such as immunoassays and immunocytochemical analysis are comparatively expensive and time-consuming and cannot analyze the targets in a living sample directly. In this paper, we developed a new fluorescence-active compound as a tool to directly study the defense signaling pathway of plant activators in plants. At first, with the active and commercial plant activators FBT and BTH as the starting materials, the two new active fluorescence plant activators N-FBT and N-BTH were designed and synthesized; the second, based on a system for the study of protein−protein interactions, a new nucleolus-tethering system (New-NoTS) especially for the study of interaction between small molecules and proteins was developed. With these two new tools, a series of studies for the defense signaling pathway of new plant activators was conducted. The results showed that N-FBT could interact with both SABP2 (SA pathway) and MYC2 (JA pathway) at the same time while N-BTH only interacted with SABP2. This means that FBT can induce the plant immune system by activating both SA and JA signaling pathways, which directly supported the extensive activities of FBT not only for disease but also for insects. On the other hand, with this method, the interactions between fluorescent plant activators and proteins could be studied directly by visualization.

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Nucleolus-tethering system (NoTS)

Cited by 2 publications

References 51 publications

Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

Active Fluorescence Plant Activator N-FBT: A New Tool for the Study of Defense Signaling Pathway in Plants

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