Nucleologenesis and Nucleolotransfer in Mammalian Oocytes: A Review

Abstract

An effort to improve development potential of early embryos is one of the main goals of biotechnology in the area of reproductive biology with application in veterinary or human medicine. Recent observations of the function of nucleolus or rather its forms before, during and after the fertilisation or parthenogenetic activation show the key role(s) of nucleolus in the processes of early genome activation. The nucleolus is a subnuclear structure (organelle) mainly involved in regulation of transcription and translation. This organelle has been characterized in detail by immunofluorescence, cell transfection and proteomics. This data was, however, mostly obtained in nucleoli of differentiated eukaryotic cells. Much less is known about the nucleolar structural changes and related functional processes in growing and fully grown mammalian oocytes, zygotes and early cleavage stage embryos, especially in the context of embryonic genome activation. It has been shown, that nucleoli in mammalian oocytes and early embryos have several forms and functions, which vary during the oocyte growth and embryonic development. Certain functions have not been fully described or explained, yet. The method of enucleolation, which allows to remove nucleoli from the oocytes or to exchange nucleoli between oocytes or zygotes, together with their proteomic and structural analyses brought new information about functions of nucleoli in oocytes and early cleavage-stage embryos and allowed to explain some new key roles of nucleoli during oocyte maturation and early embryonic development.

The Oocyte’s Nucleolus Precursor Body: The Globe for Life

“…After establishing the method of enucleolation, the field was opened again for new adventures. The method of enucleolation was described in our last review (39). The studies of Ogushi et al (10) in pig and mouse, where the oocyte's NPBs were removed and even exchanged by micromanipulation, demonstrated that the NPB may have effects on oocyte maturation and progression of meiosis and that it certainly is crucial for embryonic development.…”