Nucleoli of Diploid Cell Strains

Phillips, Stephanie Gordon; Phillips, David M.

doi:10.1083/jcb.49.3.785

Cited by 27 publications

(8 citation statements)

References 30 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1-6) . This arrangement was different from the organization in nucleoli of the nonmalignant Chinese hamster and mouse cells previously examined (Phillips and Phillips, 1971), in which the particulate and fibrillar zones were spatially separated .…”

Section: Nucleolar Structure Of Untreated Kb and L Cellsmentioning

confidence: 65%

“…The results presented in this and the companion paper (Phillips and Phillips, 1971) ated, the nucleoli of such cells appear to have the structure we have described as characteristic of KB and L cells (e .g., see Recher et al ., 1969 ;Heine, 1969 ;Martinez-Palomo and Granboulan, 1967) . On the other hand, nucleoli of "normal," nonmalignant cells of various sorts are extremely variable in morphology, and a "typical" or "normal" nucleolus cannot be defined .…”

Section: Discussionmentioning

confidence: 79%

“…In electron micrographs, the nucleoli commonly had a compact, rounded, or oval appearance (Figs . 1-6) which was quite different from the more irregular, loosely reticular appearance characteristic of nucleoli of mouse or Chinese hamster cells from 804 THE JOURNAL OF CELL BIOLOGY • VOLUME 49,1971 cultures of recent origin (Phillips and Phillips, 1971) .…”

Section: Nucleolar Structure Of Untreated Kb and L Cellsmentioning

confidence: 98%

“…Experiments were performed on the 2nd day after seeding when the cells were growing vigorously and had not yet reached confluency . Radioautography was performed as described in the companion paper (Phillips and Phillips, 1971) except that uridine-3H, SA 0 .2 Ci/mmole was use .l at a concentration of 0 .1 mCi/ml, and the radioautographs were exposed for 3 days. Techniques for electron microscopy were also as previously described (Phillips and Phillips, 1971) .…”

Section: Methodsmentioning

confidence: 99%

“…We have also found that the morphological alterations in nucleoli which are brought about by two antagonists of RNA synthesis, toyocamycin and actinomycin D, are different in the two types of cells . The fine structure of nucleoli of cell strains of nonmalignant origin (mouse and Chinese hamster fetus-derived cultures in early passages) was described in the companion paper (Phillips and Phillips, 1971) . In the present paper we contrast the nucleolar structure seen in these "normal" cells with the appearance of nucleoli in two established cell lines, one derived from a malignancy and one proven to have tumorigenic potential which is expressed upon injection into immunologically incompetent hosts.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Distinctive Characteristics of Nucleoli of Two Established Cell Lines

Phillips

1971

The Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

Nucleoli of cultured cells of the established lines KB and L were found to possess a distinctive fine structural organization. The major portion of the nucleolar volume was composed of compact, particulate material. Spheroidal fibrillar zones about 0.4 µ in diameter occurred within the particulate mass. These fibrillar zones had a central light area and a denser rim. Toyocamycin treatment, which sharply inhibited the appearance of newly synthesized RNA in the cytoplasm, caused the gradual disappearance of the fibrillar material from nucleoli. Actinomycin D treatment, which inhibited virtually all RNA synthesis, caused varying types of segregation of nucleolar components. The morphology of nucleoli of KB and L cells and the reorganization of these nucleoli in response to drugs appear to be different from those of nucleoli of freshly initiated Chinese hamster and mouse cell lines.

show abstract

Section: Nucleolar Structure Of Untreated Kb and L Cellsmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 79%

Section: Nucleolar Structure Of Untreated Kb and L Cellsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Distinctive Characteristics of Nucleoli of Two Established Cell Lines

Phillips

1971

The Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

show abstract

A Point‐by‐Point Response to Recent Arguments Against the Use of Statins in Primary Prevention

Joshi

Chaudhari

Blaha

et al. 2012

Clinical Cardiology

View full text Add to dashboard Cite

Recently, a debate over the merits of statin therapy in primary prevention was published in the Wall Street Journal. The statin opponent claimed that statins should only be used in secondary prevention and never in any primary‐prevention patients at risk for cardiovascular events. In this evidence‐based rebuttal to those claims, we review the evidence supporting the efficacy of statin therapy in primary prevention. Cardiovascular risk is a continuum in which those at an elevated risk of events stand to benefit from early initiation of therapy. Statins should not be reserved until after a patient suffers the catastrophic consequences of atherosclerosis. Contrary to the assertions of the statin opponent, this principle has been demonstrated through reductions in heart attacks, strokes, and mortality in numerous randomized controlled primary‐prevention statin trials. Furthermore, data show that once a patient tolerates the initial treatment period, the few side effects that subsequently emerge are largely reversible. Accordingly, every major guidelines committee endorses statin use in secondary prevention and selectively in primary prevention for those with risk factors. The foundation for prevention remains increased physical activity, better dietary habits, and smoking cessation. However, prevention of heart attacks, strokes, and death from cardiovascular disease does not have to be all or none—all statin or all lifestyle. In selected at‐risk individuals, the combination of pharmacotherapy and lifestyle changes is more effective than either alone. Future investigation in prevention should focus on improving our ability to identify these at‐risk individuals.Drs Amsterdam, Blumenthal, and Wong serve on the board of directors and/or are officers of the American Society for Preventive Cardiology. Dr Wong has received research support from Merck and Bristol Myers‐Squibb and advisory board consultant fees from Merck in the past 12 months. Dr Cannon is a senior investigator in the TIMI Study Group; has received research support from Accumetrics, AstraZeneca, GlaxoSmithKline, Merck, Essentialis, and Takeda; has donated advisory board consultant fees to charity from Bristol‐Myers Squibb/Sanofi, Novartis, and Alnylam; has received honoraria for development of independent educational symposia from Pfizer and AstraZeneca; and has equity in Automedics Medical Systems. Dr Jones has received research support and advisory board consultant fees from Atherotech Diagnostics Lab and has served as a consultant for Amylin Pharmaceuticals.The authors have no other funding, financial relationships, or conflicts of interest to disclose.

show abstract

Heat shock but not cold shock leads to disturbed intracellular zinc homeostasis

Pirev

Ince

Sies

et al. 2009

Journal Cellular Physiology

View full text Add to dashboard Cite

The heat shock response is a highly conserved process essential for surviving environmental stress, including extremes of temperature. To investigate whether heat shock has an impact on intracellular Zn(2+) homeostasis, cells were subjected to heat shock, and subsequently the intracellular free zinc concentration was investigated. Sublethal heat shock induced a temperature-dependent and transient intracellular Zn(2+) release that was repeatable after 24 h. The free zinc was localized in round-shaped nuclear bodies identified as nucleoli. Metallothionein, the main cellular zinc storing protein, was found to be not functionally essential for this heat-shock-induced effect. No significant oxidative stress within the cells was detected after heat shock. Cold shock and subsequent rewarming did not result in disturbed intracellular zinc homeostasis. These results show that heat shock and cold shock differ with respect to intracellular Zn(2+) release. A role for zinc as signaling ion during fever is conceivable.

show abstract

Nucleoli of Diploid Cell Strains

Cited by 27 publications

References 30 publications

Distinctive Characteristics of Nucleoli of Two Established Cell Lines

Distinctive Characteristics of Nucleoli of Two Established Cell Lines

A Point‐by‐Point Response to Recent Arguments Against the Use of Statins in Primary Prevention

Heat shock but not cold shock leads to disturbed intracellular zinc homeostasis

Contact Info

Product

Resources

About