Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases

Abstract

Abbreviations used in this paper: Ni-NTA, nickel -nitrilotriacetic acid; RNAi, RNA interference; rRNA, ribosomal RNA; SUMO, small ubiquitin-like modifi er protein; Ulp/SENP, ubiquitin-like protein/sentrin-specifi c protease; XEE, Xenopus laevis egg extract; xSENP3, Xenopus SENP3.The online version of this article contains supplemental material.

SUMO-dependent regulation of centrin-2

“…Although little is presently known about a possible role of SUMO at the centrosome, recent studies have identified several proteins that undergo SUMOylation and are known to localize to both centrosomal structures and the nuclear compartment (Cheng et al, 2006;Haindl et al, 2008;Yun et al, 2008). Our present data suggest that the SUMOylation machinery does not influence centrin-2 localization to the centrosome but is required for the efficient accumulation of the protein in the nucleus, where it functions in NER and mRNA export (Fischer et al, 2004;Resendes et al, 2008).…”

Nucleophosmin and its complex network: a possible therapeutic target in hematological diseases

“…Nucleophosmin and its complex network E Colombo et al SUMO1/sentrin/SMT3 specific peptidases 3 and 5 (SENP3-5) (Yun et al, 2008), and influences the stability of the cell cycle inhibitor p21/WAF/CIP (p21) (Xiao et al, 2009). Moreover, the chaperone activity of NPM has been linked to its ability to assist the transport of other proteins across the nuclear membrane.…”

“…Therefore, ARF and SENP3 compete to control the level of NPM sumoylation (Haindl et al, 2008;Kuo et al, 2008). However, ARF requires NPM to induce SENP3 degradation and, on the other hand, NPM is required for the correct nucleolar localization and stability of both ARF and SENP3 (Kuo et al, 2008;Yun et al, 2008). ARF and SENP3 do not seem to compete for NPM binding suggesting that NPM may provide a molecular platform in which ARF and SENP3 can meet and counteract each other's activities.…”

SUMOylation and deSUMOylation at a glance

“…SENP3 and SENP5 localize in nucleoli and catalyze SUMO-2/3 processing and deconjugation (Di Bacco et al, 2006;Gong and Yeh, 2006). Similar to Ulp1p (Panse et al, 2006), SENP3 and SENP5 have important roles in ribosome biogenesis (Yun et al, 2008). SENP6 and SENP7 localize within the nucleoplasm and are implicated in the editing of poly-SUMO chains (Lima and Reverter, 2008;Mukhopadhyay et al, 2006;Shen et al, 2009).…”