Nucleolar persistence during spermatogenesis of the genus <i>Rhodnius</i> (Hemiptera, Triatominae)

Alevi, Kaio Cesar Chaboli; Castro, Nayara Fernanda da Costa; Lima, Anna Claudia Campaner; Ravazi, Amanda; Morielle-Souza, Alessandra; Oliveira, Jader de; Rosa, João Aristeu da; Azeredo-Oliveira, Maria Tercília Vilela de

doi:10.1002/cbin.10297

Cited by 15 publications

(15 citation statements)

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“…The phenomenon of nucleolar persistence during spermatogenesis is characterized by the presence of the nucleolus or nucleolar corpuscles during meiotic metaphase, since, generally, in eukaryotes the nucleolus is fragmented in late prophase and is only reorganized at the end of prophase. This phenomenon has been described in 21 species of triatomines (Tartarotti and Azeredo-Oliveira, 1999;Azeredo-Oliveira, 2004, 2007;Severi-Aguiar and Azeredo-Oliveira, 2005;Severi-Aguiar et al, 2006;Costa et al, 2008;Alevi et al, 2013e, 2014d, and Alevi et al (2014d) have proposed that it is a synapomorphy of the subfamily Triatominae.…”

Section: Discussionmentioning

confidence: 86%

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

Pereira¹,

Alevi²,

Mendonca³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study describes spermatogenesis in Triatoma vandae and the nucleolar behavior of T. vandae and Triatoma williami, with a cytotaxonomic focus. Analysis of mitotic and meiotic metaphases of T. vandae confirms the species karyotype. T. vandae presents some characteristics during meiosis that differentiate it from T. williami, including the presence of a chromocenter with two sex chromosomes individualized during early prophase, and the presence of a bi-or tripartite corpuscle in polyploid nuclei. It was possible to observe the compaction of chromatin during prophase resulting in holocentric chromosomes. During metaphase, the autosomes presented a ring shape and the sex chromosomes were in the center of the ring. These chromosomes were separated in anaphase. Although it is common, we did not observe the phenomenon of late migration of the sex chromosomes. By means of silver ion impregnation it was possible to describe nucleologenesis in T. vandae and T. williami.

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Section: Discussionmentioning

confidence: 86%

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

Pereira¹,

Alevi²,

Mendonca³

et al. 2015

Genet. Mol. Res.

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“…The 148 species currently described (Abad-Franch et al, 2013;Alevi et al, 2013a;Jurberg et al, 2013;Poinar Jr., 2013) are potential vectors of the protozoan Trypanosoma cruzi, the etiologic agent of Chagas disease. Triatomines are also of biological interest because their cells have several peculiarities in comparison to cells of other eukaryotes, such as holocentric chromosomes (Panzera et al, 1996), inverted meiosis for sex chromosomes (Gómez-Palacio et al, 2008), and persistence of nucleolar material during meiosis (Tartarotti and Azeredo-Oliveira, 1999;Alevi et al, 2014a). In addition, triatomines are of evolutionary interest because their origins (monophyletic or polyphyletic) have not been conclusively determined (Tartarotti et al, 2006;Hwang and Weirauch, 2012).…”

Section: Introductionmentioning

confidence: 99%

Description of the diploid chromosome set of Triatoma pintodiasi (Hemiptera, Triatominae)

Alevi¹,

Moreira

Jurberg

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Triatoma pintodiasi has been described and recently grouped in the Rubrovaria subcomplex. T. pintodiasi was initially compared to T. carcavalloi by staining and subsequently identified as T. circummaculata. However, after thorough examination, it was observed to be a cryptic species of T. circummaculata, and was described based on morphological features, morphometric data, and biochemical patterns of hemolymph. Thus, this paper aims to describe the karyotype of, and spermatogenesis in, T. pintodiasi, in order to elucidate the reproductive biology and taxonomy of the species. Sex chromosomes of T. pintodiasi formed a heteropyknotic chromocenter, and compaction of chromatin was observed during prophase. However, in contrast to observations in T. carcavalloi and T. circummaculata, in T. pintodiasi it was observed individualization of the sex chromosomes. The diploid chromosome set of the species 2n = 22 (20A + XY) is described through analysis of metaphases I and II. Initial cytogenetic characteristics of T. pintodiasi are described and the observed differences in the chromocenter

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“…Recently, Alevi and others 9 have observed that all species within the genus Rhodnius feature nucleolar persistence during meiosis as well, and they suggested that analyses of the nucleolar behavior should be carried out in a large range of species of triatomines to examine whether that characteristic is a synapomorphy of the Triatominae subfamily.…”

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confidence: 99%

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Madeira

Borsatto

Lima

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. All species of triatomines are considered potential vectors of Chagas disease and the reproductive biology of these bugs has been studied by different approaches. In 1999, nucleolar persistence during meiosis was observed in the subfamily for the first time. Recently, it has been observed that all species within the genus Rhodnius exhibit the same phenomenon, suggesting that it may be a synapomorphy of the triatomines. Thus, this article aims to analyze the nucleolar behavior during spermatogenesis of 59 triatomine species. All analyzed species exhibited nucleolar persistence during meiosis. Recently, it has been suggested that nucleolar persistence may be fundamental for the spermatogenesis of these vectors, since it is related to the formation of the chromatoid body. Therefore, we emphasize that this phenomenon is a peculiarity of the Triatominae subfamily and that further studies are required to analyze whether the nucleolar material that persists is active.The Triatominae subfamily is composed of 150 species (148 living species and two fossils), grouped into 18 genera and five or six tribes. All triatomine species are considered potential vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease. 1Chagas disease is a neglected disease that has no cure. The main way to minimize the incidence of this disease in human populations is through vector control.1 It is estimated that more than 5 million people are infected by T. cruzi, the parasite that causes Chagas disease, and that 70 million still live at risk, which places this illness among the most serious parasitic diseases in the world.1 Thus, improving the knowledge on several fields on triatomine vector potentiality (such as ecology 2 and biology 3 ) may provide important information for control measures.The reproductive biology of these bugs has been studied by different approaches, such as cytogenetic, 4 structural, 5 and ultrastructural 6 analysis. Furthermore, the spermatogenesis of the triatomines is characterized as cystic, 5,6 and it has been suggested that, during imaginal molt (transition from the fifth instar nymph to adult), the cell division is disrupted, aiming to reduce energy costs, and the differentiation into sperm is stimulated to ensure the paternity of the adult male. 7In 1999, Tartarotti and Azeredo-Oliveira, 8 while studying the spermatogenesis of Panstrongylus megistus and Panstrongylus herreri (= Panstrongylus lignarius), noted that these triatomines exhibited a different nucleolar behavior than the one described for other eukaryotes: the nucleolus persisted during all stages of meiosis. The authors characterized this phenomenon as nucleolar persistence.Recently, Alevi and others 9 have observed that all species within the genus Rhodnius feature nucleolar persistence during meiosis as well, and they suggested that analyses of the nucleolar behavior should be carried out in a large range of species of triatomines to examine whether that characteristic is a synapomorphy of the Triatominae subfamily.Thus, this ...

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Nucleolar persistence during spermatogenesis of the genus Rhodnius (Hemiptera, Triatominae)

Cited by 15 publications

References 20 publications

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

Description of the diploid chromosome set of Triatoma pintodiasi (Hemiptera, Triatominae)

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

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