Nucleolar organizer regions as markers of endometrial proliferation: A study of normal, hyperplastic, and neoplastic tissue

Brustmann, Hermann; Riss, P.; Naudé, S. M.

doi:10.1016/0046-8177(95)90173-6

Cited by 14 publications

(15 citation statements)

References 23 publications

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“…The counting of AgNORs may be a method of assessing cellular proliferation [30]. It also has a diagnostic significance between neoplastic and preneoplastic lesions, including uterine endometrium [21,24,25]. Despite its limitations mainly related to section thickness and tedious, time-consuming counting, the silver staining NOR technique represents a potential tool for the indirect evaluation of cell kinetics in already routinely fixed and paraffin-embedded sections.…”

Section: Discussionmentioning

confidence: 99%

“…Despite its limitations mainly related to section thickness and tedious, time-consuming counting, the silver staining NOR technique represents a potential tool for the indirect evaluation of cell kinetics in already routinely fixed and paraffin-embedded sections. It may appear very useful in retrospective studies [21,24,25].…”

Section: Discussionmentioning

confidence: 99%

“…In several reports investigating AgNOR counts in endometrial lesions, significant differences have been noted between normal or hyperplastic endometrium and endometrial adenocarcinoma [21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

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Nucleolar Organizer Regions in Differentiated Preneoplastic and Neoplastic Endometrial Lesions

Terlikowski

Lenczewski²,

Sulkowski³

et al. 1999

Gynecol Obstet Invest

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The aim of the study was the quantitative assessment of nucleolar organizer regions (NORs) in the cells of preneoplastic and neoplastic endometrial lesions. Retrospective analysis included tissue material obtained from 87 patients with simple and complex hyperplasia with or without atypia and well-differentiated stage-I endometrial adenocarcinoma. Sections were made from routine paraffin blocks and stained with AgNO₃. The mean number of AgNORs per cell was determined. The results obtained indicate that the evaluation of proliferative activity of cells expressed by AgNOR count can help in the distinction between atypical hyperplasia and well-differentiated adenocarcinoma, and thus can serve as a useful pathological criterion.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Nucleolar Organizer Regions in Differentiated Preneoplastic and Neoplastic Endometrial Lesions

Terlikowski

Lenczewski²,

Sulkowski³

et al. 1999

Gynecol Obstet Invest

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“…Those studies demonstrated that the number of argyrophilic regions is high in malignant tumours including brain tumours and that high counts can roughly reflect a grade of malignancy or differentiate among reactive, benign, and malignant conditions (Brustmann et al, 1995). It has been suggested that the argyrophilic region count reflects ploidy and/or proliferative activity (Derenzini et al, 1990;Suresh et al, 1990).…”

Section: Functional Significance Of Argyrophilic Nucleolar Organizer mentioning

confidence: 99%

A combined staining method for argyrophilic nucleolar organizer regions and for glial fibrillary acidic protein in astrocytes of human brain

et al. 1996

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Different protocols are described for the combined staining method by which argyrophilic nucleolar organizer region sites can be evaluated in human astrocytes that are immunoreactive for glial fibrillary acidic protein. Among the four protocols studied, the following method was superior to others in terms of unambiguous visualization of the regions in glial fibrillary acidic protein-positive astrocytes; the first step was immunostaining for the protein with a blue colour reaction of alkaline phosphatase, followed by sequential colloidal silver staining for the regions. By this double staining method, we have demonstrated that the reactive astrocytes found in white matter around the metastatic lesion of carcinoma and the infarction, contain more argyrophilic nucleolar organizer regions in terms of the count as well as the area than glial fibrillary acidic protein-positive astrocytes present in the white matter of the normal brain. In conclusion, the double staining may provide valuable information on the cellular activity of astroglia when performed on routine formalin-fixed paraffin sections of the human brain.

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“…NORs can be demonstrated by a simple argyrophilic staining technique, the Ag (argyrophilic)-NOR method [25], which stains NOR associated proteins because they bind silver ions selectively [5,18]. Thus the site and number of NORs can be visualized in chromosome spreads [2], whole cells [3] and histological sections [4,6]. The relationship of AgNOR to proliferative activity and grade of malignancy has been the subject of investigation in clinical pathology [6,10,11,21,27,29].…”

Section: Introductionmentioning

confidence: 99%

Age-Related Changes of the Argyrophilic Nucleolar Organizer Regions in Mouse Neocortical Neurons.

Fushiki

Kinoshita

Tsutsumi

et al. 1995

Acta Histochem. Cytochem

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Nucleolar organizer regions as markers of endometrial proliferation: A study of normal, hyperplastic, and neoplastic tissue

Cited by 14 publications

References 23 publications

Nucleolar Organizer Regions in Differentiated Preneoplastic and Neoplastic Endometrial Lesions

Nucleolar Organizer Regions in Differentiated Preneoplastic and Neoplastic Endometrial Lesions

A combined staining method for argyrophilic nucleolar organizer regions and for glial fibrillary acidic protein in astrocytes of human brain

Age-Related Changes of the Argyrophilic Nucleolar Organizer Regions in Mouse Neocortical Neurons.

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