Nucleobasen in der Gasphase

Abstract

Das Problem der thermischen Instabilität von Nucleobasen kann durch Anwendung von Laserablation oder ‐desorption überwunden werden. Zwei neuartige Techniken, die Laserablation/Überschallstrahl‐Mikrowellenspektroskopie und die ultraschnelle Elektronenbeugung/Laserdesorption, ermöglichten so die strukturelle Charakterisierung von Guanin (siehe Bild; die Pfeildicke gibt die Stabilität des Konformers an).

All Five Forms of Cytosine Revealed in the Gas Phase

“…The values of the inertial defects show that all species are effectively planar. The superior quality of this technique for the determination of different conformational/tautomeric forms of nucleic acid bases has been discussed previously 22. In the present study of cytosine, we were able to detect keto–imine forms predicted to be more than 1200 cm −1 (in Gibbs values) above the global minimum.…”

Conformational Preferences of Gas‐Phase Helices: Experiment and Theory Struggle to Agree: The Seven‐Residue Peptide Ac‐Phe‐(Ala)5‐Lys‐H+

“…The physical chemistry of biomolecules in the gas phase is progressing at a rapid pace 1–5. One of the most promising areas is the determination of the conformations of biologically important molecules 6.…”