Nucleic Acids: Hydration

Chalikian, Tigran V.; Völker, Jens

doi:10.1002/9780470048672.wecb407

Cited by 4 publications

(5 citation statements)

References 54 publications

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“…According to Le Châtelier's principle, this leads to an overall decrease in the volume of the system upon dissociation of the droplet phase by pressure (Figure 5), which is also favored by a higher mixing entropy. Differential changes in protein surface hydration could make an additional contribution, [40] which is probably of minor importance here. Of note, void volumes can also arise from imperfect packing in folded structures of globular proteins, but these unfold at much higher pressures, typically between ∼4 and ∼8 kbar [39] …”

Section: Discussionmentioning

confidence: 92%

Suppression of Liquid‐Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application

Fetahaj

Jaworek

Oliva

et al. 2022

Chemistry A European J

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The high colloidal stability of antibody (immunoglobulin) solutions is important for pharmaceutical applications. Inert cosolutes, excipients, are generally used in therapeutic protein formulations to minimize physical instabilities, such as liquid–liquid phase separation (LLPS), aggregation and precipitation, which are often encountered during manufacturing and storage. Despite their widespread use, a detailed understanding of how excipients modulate the specific protein‐protein interactions responsible for these instabilities is still lacking. In this work, we demonstrate the high sensitivity to pressure of globulin condensates as a suitable means to suppress LLPS and subsequent aggregation of concentrated antibody solutions. The addition of excipients has only a minor effect. The high pressure sensitivity observed is due to the fact that these flexible Y‐shaped molecules create a considerable amount of void volume in the condensed phase, leading to an overall decrease in the volume of the system upon dissociation of the droplet phase by pressure already at a few tens of to hundred bar. Moreover, we show that immunoglobulin molecules themselves are highly resistant to unfolding under pressure, and can even sustain pressures up to about 6 kbar without conformational changes. This implies that immunoglobulins are resistant to the pressure treatment of foods, such as milk, in high‐pressure food‐processing technologies, thereby preserving their immunological activity.

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Section: Discussionmentioning

confidence: 92%

Suppression of Liquid‐Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application

Fetahaj

Jaworek

Oliva

et al. 2022

Chemistry A European J

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“…Previous studies have indicated that 10 to 30 water molecules per phosphate are involved in the interaction with DNA 30 . The hydrating properties of nucleic acids suggest that PN can be beneficial for individuals with dry skin that has weakened scaffolding.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have indicated that 10 to 30 water molecules per phosphate are involved in the interaction with DNA. 30 The hydrating properties of nucleic acids suggest that PN can be beneficial for individuals with dry skin that has weakened scaffolding. Also, PN serve as both barrier organelles and structural scaffolds that support the proper accumulation of ECM during the process of wound healing.…”

Section: Discussionmentioning

confidence: 99%

Current practices and perceived effectiveness of polynucleotides for treatment of facial erythema by cosmetic physicians

Lee,

Kim,

Park

et al. 2023

Skin Research and Technology

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BackgroundPolynucleotides (PN) are increasingly used for the treatment of facial erythema in the Republic of Korea. However, there are limited pre‐clinical and clinical data on the efficacy of polynucleotides for facial erythema. In this study, we investigated the current practice and perceived effectiveness of polynucleotide treatment for facial erythema among cosmetic physicians.MethodsBy conducting a survey among clinicians who use PN in clinical practice, we explored the current practices and assessed the perceived effectiveness of polynucleotides in treating facial erythema.ResultsA total of 557 physicians who used polynucleotides for facial erythema participated in the survey. Polynucleotides were used by 84.4%, 66.4%, and 47.4% of physicians for facial erythema caused by inflammatory facial dermatosis, repeated laser/microneedle radiofrequency, and steroid overuse, respectively. Among those users, 88.1%, 90%, and 83.7% respectively in those same categories answered that polynucleotides were “highly effective” or “effective.” Furthermore, they agreed that polynucleotides have the following properties: wound healing/regeneration (95.8%), protection of skin barrier (92.2%), hydration (90.5%), vascular stabilization (81.0%), and anti‐inflammation (79.5%).ConclusionOur findings showed that cosmetic physicians in the Republic of Korea have used PN as a part of combination treatment for facial erythema resulting from inflammatory facial dermatosis and repeated laser/ microneedle radiofrequency, rather than from steroid overuse. Also, most clinicians agreed that PN was effective for treatment of facial erythema. Given the lack of pre‐clinical and clinical trial evidence, the empirical responses of practicing physicians provide useful information to guide clinical practice and further research.

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“…This result can be explained by differences in the hydration of A-DNA and B-DNA. It is known that the A-form of DNA is formed in lower hydration conditions than B-form DNA [29][30][31][32][33][34]. However, in A-DNA the main hydrated water molecules are concentrated on strongly polar oxygens of phosphate groups and form water chains along the DNA chain [31,32].…”

Section: The Thermodynamics Of Binding Of Porphyrins With A-and B-formentioning

confidence: 99%

“…Water bound on phosphate groups does not form a similar lattice in the major groove of B-DNA. The B-form of DNA is stabilized by a water backbone formed by water molecules from the first hydration layer of AT-rich regions of the DNA minor groove [33,34]. Therefore it can be concluded that the major groove of A-DNA is hydrated more intensly than the major groove of B-DNA.…”

Section: The Thermodynamics Of Binding Of Porphyrins With A-and B-formentioning

confidence: 99%

Thermodynamics of interaction ofmeso-tetra-(4N-oxyethylpyridyl) porphyrin and its Cu(II)- and Co(II)- containing derivatives with A and B forms of DNA

Avetisyan

Vardanyan

Dalyan

2017

J. Porphyrins Phthalocyanines

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ABSTRACT:The interaction of water soluble meso-tetra-(4N-oxyethylpyridyl) porphyrin (TOEPyP4) and its Cu(II)-and Co(II)-containing derivatives (CuTOEPyP4 and CoTOEPyP4) with A and B forms of DNA at low ionic strength was studied via UV-vis spectrophotometry and Circular Dichroism. It is shown that the binding constant of TOEPyP4 and CuTOEPyP4 with A-DNA is two times larger than with B-DNA, and the binding constant of CoTOEPyP4 does not depend on the form of DNA. The thermodynamical analysis based on spectral data indicates the preferable entropic character of porphyrins binding with both forms of DNA. This result shows that at low ionic strength the external groove binding mode is a preferred binding mechanism of these porphyrins with both forms of DNA.

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Nucleic Acids: Hydration

Cited by 4 publications

References 54 publications

Suppression of Liquid‐Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application

Suppression of Liquid‐Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application

Current practices and perceived effectiveness of polynucleotides for treatment of facial erythema by cosmetic physicians

Thermodynamics of interaction ofmeso-tetra-(4N-oxyethylpyridyl) porphyrin and its Cu(II)- and Co(II)- containing derivatives with A and B forms of DNA

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