Nucleic Acid Testing for Coronavirus Disease 2019: Demand, Research Progression, and Perspective

Zhang, Shiyin; Su, Xiaosong; Wang, Jin; Li, Caiyu; Li, Tingdong; Ge, Shengxiang; Xia, Ningshao

doi:10.1080/10408347.2020.1805294

Cited by 30 publications

(25 citation statements)

References 61 publications

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“…As the ddPCR instrument parts, including the droplet generation instruments, thermocyclers, and droplet reader, usually have a complex structure, a single device for digital PCR detection has been designed with solar power for absolute quantitative detection of the hepatitis B virus in human serum. 167 Portable rapid methods are one of the growing fields of interest in molecular detection. Affordable POCT devices are effective tools to monitor epidemics such as the coronavirus outbreak of 2019.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Kojabad

Farzanehpour

Galeh

et al. 2021

Journal of Medical Virology

135

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High‐throughput droplet‐based digital PCR (ddPCR) is a refinement of the conventional polymerase chain reaction (PCR)‎ methods. In ddPCR, DNA/RNA is encapsulated stochastically inside the microdroplets as reaction chambers. A small percentage of the reaction chamber contains one or fewer copies of the DNA or RNA. After PCR amplification, concentrations are determined based on the proportion of nonfluorescent partitions through the Poisson distribution. Some of the main features of ddPCR include high sensitivity and specificity, absolute quantification without a standard curve, high reproducibility, good tolerance to PCR inhibitor, and high efficacy compared to conventional molecular methods. These advantages make ddPCR a valuable addition to the virologist's toolbox. The following review outlines the recent technological advances in ddPCR methods and their applications in viral identification.

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Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Kojabad

Farzanehpour

Galeh

et al. 2021

Journal of Medical Virology

135

View full text Add to dashboard Cite

show abstract

“…The detection of viral nucleic acids at the early stage of COVID-19 infection presents a higher sensitivity and specificity compared to antigen or antibody detection [37]. In general, nucleic acid testing involves three main steps: nucleic acid extraction, amplification, and detection [122][123][124]. Most COVID-19 tests have been developed to detect the SARS-CoV-2 specific genes including the RNA-dependent RNA polymerase (RdRP) gene, Nucleocapsid (N) gene, Envelope (E) protein gene, Spike protein (S) gene, and ORF1ab gene [125,126].…”

Section: Poc Biosensors For the Detection Of Nucleic Acids For Covid-19mentioning

confidence: 99%

Emerging point-of-care biosensors for rapid diagnosis of COVID-19: current progress, challenges, and future prospects

Rasmi

Khan

et al. 2021

Anal Bioanal Chem

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Coronavirus disease 2019 (COVID-19) pandemic is currently a serious global health threat. While conventional laboratory tests such as quantitative real-time polymerase chain reaction (qPCR), serology tests, and chest computerized tomography (CT) scan allow diagnosis of COVID-19, these tests are time-consuming and laborious, and are limited in resource-limited settings or developing countries. Point-of-care (POC) biosensors such as chip-based and paper-based biosensors are typically rapid, portable, cost-effective, and user-friendly, which can be used for COVID-19 in remote settings. The escalating demand for rapid diagnosis of COVID-19 presents a strong need for a timely and comprehensive review on the POC biosensors for COVID-19 that meet ASSURED criteria: Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Deliverable to end users. In the present review, we discuss the importance of rapid and early diagnosis of COVID-19 and pathogenesis of COVID-19 along with the key diagnostic biomarkers. We critically review the most recent advances in POC biosensors which show great promise for the detection of COVID-19 based on three main categories: chip-based biosensors, paper-based biosensors, and other biosensors. We subsequently discuss the key benefits of these biosensors and their use for the detection of antigen, antibody, and viral nucleic acids. The commercial POC biosensors for COVID-19 are critically compared. Finally, we discuss the key challenges and future perspectives of developing emerging POC biosensors for COVID-19. This review would be very useful for guiding strategies for developing and commercializing rapid POC tests to manage the spread of infections.

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“…According to WHO recommendations, tests based on molecular biology techniques are recommended for the diagnosis of acute SARS-CoV-2 infections. Currently, realtime reverse transcriptase polymerase chain reaction (RT-qPCR) is the most commonly used and preferred method for the coronavirus testing due to its sensitivity, specificity, and robustness [57]. The RT-qPCR is used for the detection of almost all the coronaviruses including SARS-CoV-2.…”

Section: Sars-cov-2 and Coronavirus Disease 2019 (Covid-19)mentioning

confidence: 99%

Coronaviruses – a new old menace

2021

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current pandemic caused by novel coronavirus SARS-CoV-2 pandemic has been described as a global health emergency. The outbreak of this virus has raised a number of questions: what exactly is SARS-CoV-2? How transmissible the novel coronavirus is? How severely affected are patients infected with SARS-CoV-2? What are the risk factors for COVID-19? What are the differences between this novel coronavirus and other coronaviruses? To answer these questions, a comparative study of three pathogenic coronaviruses that primarily invade the human respiratory system and may cause death, namely, severe acute respiratory syndrome (SARS-CoV-1), Middle East respiratory syndrome (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Middle East respiratory syndrome coronavirus (MERS-CoV). This review describes the source of origin, transmission, and pathogenicity of these viruses. Prevention of SARS-CoV-2 spreading entails home isolation of suspected cases and those with mild illnesses and strict infection control measures at hospitals that include contact and droplet precautions. The novel coronavirus spreads faster than its two predecessors – the SARS-CoV-1 and MERS-CoV – but has lower fatality rate. The global impact of this new pandemic is still uncertain, but it is a challenge to healthcare systems around the world.

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Nucleic Acid Testing for Coronavirus Disease 2019: Demand, Research Progression, and Perspective

Cited by 30 publications

References 61 publications

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Emerging point-of-care biosensors for rapid diagnosis of COVID-19: current progress, challenges, and future prospects

Coronaviruses – a new old menace

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