Nucleic Acid Synthesis in Mustard Gas-Treated E. Coli B

Herriott, Roger M.

doi:10.1085/jgp.34.6.761

Cited by 41 publications

(5 citation statements)

References 3 publications

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“…As a result the cells enlarge but lose their ability to divide and eventually die possibly as a consequence of "unbalanced growth," (Barner and Cohen, 1954). This observed pattern, whereby DNA synthesis is inhibited while RNA and protein synthesis continues, has been reported for E. coli subjected to ultraviolet (Kanazir and Errera, 1954;Kelner, 1953) and ionizing radiations (Doudney, 1956), nitrogen mustards (Harold and Ziporin, 1958) and sulfur mustards (Harold and Ziporin, 1958;Herriott, 1951;Pardee, 1954).…”

Section: Discussionsupporting

confidence: 67%

EFFECTS OF 5-BROMODEOXYCYTIDINE IN ESCHERICHIA COLI AND BACTERIOPHAGE

1960

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The inhibitory effects of 5-bromodeoxycytidine and 5-chlorodeoxycytidine upon growth and viability of Escherichia coli strain 15T-, a thymine requiring mutant, was described in a previous communication (Frisch and Visser, 1959). It was also reported that 5-bromodeoxycytidine produced a high frequency of plaque-type mutations in T2 bacteriophage. It has been recently demonstrated (Bessman et al., 1958) that 5bromocytosine partially replaces cytosine of deoxyribonucleic acid (DNA) formed in a cell-free

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Section: Discussionsupporting

confidence: 67%

EFFECTS OF 5-BROMODEOXYCYTIDINE IN ESCHERICHIA COLI AND BACTERIOPHAGE

1960

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“…This was interpreted as indicating development of the phage. Herriot (1951) found that nitrogen mustard inhibited desoxyribose nucleic acid synthesis more than ribose nucleic acid synthesis in E. coli. It would be of interest to know the effect of reactivating light on this reaction.…”

Section: Discussionmentioning

confidence: 96%

Growth, Respiration, and Nucleic Acid Synthesis in Ultraviolet-Irradiated and in Photoreactivated Escherichia Coli

Kelner

1953

J Bacteriol

186

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Irradiation of the cell with wavelengths (circa 2,600 A) of ultraviolet light strongly absorbed by nucleic acids can result in profound cellular changes, e.g., inhibition of growth, or of adaptive enzyme formation; in increased mutation or in death. Visible and near-visible light (from about 3,600 to 4,900 A), henceforth referred to as reactivating light, when applied to the cell after ultraviolet irradiation, will prevent death of the cell (a phenomenon called photoreactivation, Kelner, 1949a, 1949b, 1951) or reduce the frequency of induced mutants among survivors (Kelner, 1949b; Novick and Szilard, 1949; Newcombe and Whitehead, 1951; Kimball and Gaither, 1951; Brown, 1951); reduce ultravioletinduced inhibition of growth (Blum et al., 1950; Blum and Matthews, 1952; Wells and Giese, 1950); prevent the inhibition of adaptive enzyme formation (Swenson and Giese, 1950); prevent injury to cells of higher plants (Bawden and Kleezkowski, 1952); prevent changes in the morphology of the nucleoli in grasshopper neuroblats (Carlson and McMaster, 1951). The multiplicity of the biological effects of ultraviolet radiation, and the fact that most of them are reversed by reactivating light, suggests the theory that ultraviolet causes some basic change in the cell, which in turn causes the various other biological effects, usually after some delay. Reactivating light, by reversing this basic change, prevents the other effects (mutation, death, inhibition of adaptive enzyme formation, etc.). Even if this theory is not correct, it would be helpful to find some ultraviolet-induced reaction which is affected by reactivating light, and 1 Aided in part by a grant from the American Cancer Society. 2 This work was done during the tenure of a

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“…Experimental Details Preparation of _Phage.--The methods of preparing, purifying (17), and fitering (39) phage have already been discussed.…”

Section: Discussionmentioning

confidence: 99%

The Protein Coats or "Ghosts" of Coliphage T2

Herriott

Barlow

1957

The Journal of General Physiology

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With the discovery (1) that the protein coat or "ghost" of coli bacteriophage T2 carried some of the biological functions of the parent virus, it was possible to examine more carefully than heretofore the relationship of biological function to morphological structure and chemical constitution. In the series of papers to follow data will be given to support the statements in the original report (1) and to demonstrate that many properties usually associated with the intact virus are found in the protein component, suggesting a high degree of specialization and organization in this relatively small biological unit.In this, the first, paper will be described the preparation, fractionation, method of assay, and some physicochemical properties of the ghosts of T2 phage. The biological properties of the ghosts will be described in the second paper while studies of the metabolic changes induced in the host cell by ghosts (2, 3) will appear in the third and fourth papers. The inhibitory effect of divalent cations (4) on the action of ghosts will be presented in the fifth paper of this series.In the interim since the first report a number of papers (5-14) have dealt to a varying degree with some of the properties of ghosts. These will be discussed in the text of this and succeeding papers. Materials and MethodsMany of the materials and methods used in the present work are the usual ones (15, 16) described for the T phages of Escherichia coli B. The details are found either in the protocols or in the section on Experimental Details near the end of this paper.Only highly purified phage (titer/E40o > 2) (17) was used in the present experiments. In most instances freshly shocked but unfractionated ghosts were used but in certain critical experiments the results were confirmed with ghosts freed of nucleic acid as described in Table I. These instances are noted in the protocols.

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Nucleic Acid Synthesis in Mustard Gas-Treated E. Coli B

Cited by 41 publications

References 3 publications

EFFECTS OF 5-BROMODEOXYCYTIDINE IN ESCHERICHIA COLI AND BACTERIOPHAGE

EFFECTS OF 5-BROMODEOXYCYTIDINE IN ESCHERICHIA COLI AND BACTERIOPHAGE

Growth, Respiration, and Nucleic Acid Synthesis in Ultraviolet-Irradiated and in Photoreactivated Escherichia Coli

The Protein Coats or "Ghosts" of Coliphage T2

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