Nucleic Acid Similarities Among Pseudomonas pseudomallei, Pseudomonas multivorans , and Actinobacillus mallei

Abstract

Annealing experiments on membrane filters were carried out with deoxyribonucleic acids (DNA) from selected strains of the nomen-species of Pseudomonas, Actinobacillus, Chromobacteriwn, and Micrococcus, with the use of DNA of Pseudomonas pseudomallei and Actinobacillus mallei as reference materials. Under the usual conditions employed in these experiments, the results were not quantitatively reproducible. Incorporation of dimethylsulfoxide (DMSO) into the incubation medium greatly increased differences in comparative binding. DNA binding in agar matrices was examined in the presence and absence of DMSO at various incubation temperatures. It was found that the greatest specificity, stability, and total binding for DNA containing high amounts of guanine and cytosine occurred in the presence of DMSO. Under the most stringent annealing conditions permitted in agar, DNA species from P. pseudomallei and A. mallei in the presence of DMSO demonstrated interspecific relative bindings of 76 to 86% when compared to the homologous reactions. The thermal elution midpoints (Em) of these duplexed interspecific DNA species were quite close to the homologous Em values. The relative bindings of P. multivorans DNA types to either reference DNA ranged between 6 to 27%, and the Em values were 4 to 7 C less than those for the homologous reactions. were extracted from these organisms and characterized by composition and homologous and heterologous annealing abilities.

A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene

“…B. mallei and B. pseudomallei were separated into two species but nowadays they are considered to be pathovars of only one species due to DNA^DNA hybridisation studies [23,37]. But only few genes have been compared so far.…”

“…Therefore, it was of no surprise that DNA^DNA homology studies revealed that both species are simply pathovars of one single species. However, the nomenclature remained unchanged [23,37]. Recently, it was noted that in the entity of the soil isolates of B. pseudomallei various apathogenic, arabinose-fermenting strains exist [5,25,29,33,36].…”

Diversity and significance of Burkholderia species occupying diverse ecological niches

“…Burkholderia pseudomallei is a saprophytic organism that routinely can be isolated from environmental niches like water, moist soil and rice paddies (Brook et al, 1997). These two species were shown to represent a single genomic species by DNA-DNA hybridization criteria (Rogul et al, 1970). However, the major differences in their biochemical activities and in the clinical symptoms and epidemiology justify this two-species concept.…”

Gas chromatography-mass spectrometry method for rapid identification and differentiation ofBurkholderia pseudomalleiandBurkholderia malleifrom each other,Burkholderia thailandensisand several members of theBurkholderia cepaciacomplex

“…This has been demonstrated in part by comparing their cellular lipid and fatty acid compositions (Rogul et al . ; Yabuuchi et al . ; Godoy et al .…”