Nucleic Acid Independent Synthesis of Peptides

Kleinkauf, Horst; Döhren, Hans von

doi:10.1007/978-3-642-68058-8_6

Cited by 22 publications

(8 citation statements)

References 153 publications

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“…54 This can be attributed to a lack of the sophisticated "multiple sieving" selectivities of the aminoacyl tRNA synthetases and the ribosome itself. 55 The enormous selective pressure to develop very high fidelity translation for proteins early in evolution would not be expected to operate as effectively for nonribosomal peptide synthesis.…”

Section: A Initial Observationsmentioning

confidence: 99%

The Mechanism of ACV Synthetase

Byford¹,

Baldwin²,

Shiau³

et al. 1997

Chem. Rev.

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Section: A Initial Observationsmentioning

confidence: 99%

The Mechanism of ACV Synthetase

Byford¹,

Baldwin²,

Shiau³

et al. 1997

Chem. Rev.

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“…In most cases, these antibiotics are synthesized from enzymatic complexes rather than from ribosome-dependent systems (14,(25)(26)(27). Extensive biochemical studies indicated that these complexes consist of several large polypeptides, ranging in molecular mass from 100 to 440 kilodaltons (kDa) and containing many thiolactive catalytic sites (17). Collectively, these complexes serve as a template to activate amino acids, racemize amino acids from L to D forms, and direct their sequential condensation to form the peptide antibiotic; the enzymology of this unique biochemical process has been extensively reviewed (16,18,19,24).…”

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confidence: 99%

Streptomyces genes involved in biosynthesis of the peptide antibiotic valinomycin

et al. 1990

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We have identified genes from Streptomyces kvoris A-9 involved in the biosynthesis of the peptide antibiotic valinomycin. Two segments of chromosomal DNA were recovered from genomic libraries, constructed by using the low-copy-number plasmid pUJ922, by complementation of valinomycin-deficient (vlm) mutants of S. levoris A-9. One set of plasmids restored valinomycin production to only one mutant, that carrying vim-l, whereas a second set of plasmids restored productivity to seven vim mutants, those carrying vim-2 through vlm-8.Additional complementation studies using subcloned restriction enzyme fragments showed that the vlm-P + gene was contained within a 2.5-kilobase (kb) DNA region, whereas alleles vim-2+ through vlm-8+ were contained in a 12-kb region, representing at least three genes. Physical mapping experiments based on the isolation of cosmid clones showed that the two vlm loci were 50 to 70 kb apart. Southern hybridization experiments demonstrated that the vlm-2+ gene cluster was highly conserved among other valinomycin-producing

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“…of, e.g., cyclosporins, destruxins, enniatins, actinomycins, and quinoxaline antibiotics. These peptides or depsipeptides, which often contain unusual amino acids not present in proteins, are synthesized via nonribosomal pathways (Kleinkauf & von Dohren, 1981). The enzyme systems responsible for the formation of these metabolites obviously exhibit an Nmethyltransferase function since in no case were N-methylated 0006-2960/87/0426-8417S01.50/0 © 1987 American Chemical Society…”

mentioning

confidence: 99%