Nucleic Acid Hybridization Studies and Deoxyribonucleic Acid Base Compositions of Anaerobic, Gram-Positive Cocci

Abstract

Deoxyribonucleic acid-deoxyribonucleic acid reassociation and deoxyribonucleic acid-ribosomal ribonucleic acid cistron similarity studies showed that the anaerobic, gram-positive cocci comprise a rather heterogeneous group of bacteria. The deoxyribonucleic acid-ribosomal ribonucleic acid hybridization studies distinguished seven groups. Groups 1 and 2 consisted of Peptostreptococcus magnus and Peptostreptococcus prevotii, respectively. Peptostreptococcus asaccharolyticus ATCC 14963T (T = type strain) and Peptostreptococcus indolicus ATCC 29427T formed a third group, and Peptostreptococcus asaccharolyticus DSM 20364 together with Hare group VIII strain NCTC 9820 formed group 4. Peptostreptococcus anaerobius DSM 20357 was more closely related to Eubacterium tenue ATCC 25553T and Clostridium lituseburense ATCC 25759T than to any of the other species studied. Peptostreptococcus micros strains DSM 2046gT and DSM 20367 together with strains belonging to Hare group IX formed group 6, and group 7 consisted of Peptostreptococcus pawulus DSM 20469T. Strains of different Hare groups were all assigned to one of the seven groups or to the genus Staphylococcus (Hare group VIIb strain NCTC 9819) or the genus Streptococcus (Hare group VIa strain NCTC 9806). The anaerobic cocci also have a diversity of murein structures. This is the first report showing that strains belonging to the same species have different murein types.

The differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis

“…preuotii, in agreement with Huss et aZ. 5 Again, the strains were saccharolytic and the true grouping could be more in relation to P . tetradius (Gaflya anaerobia) of Ezaki et al 4 but we made no direct comparisons.…”

Bacterial Artificial Chromosome Library of Finegoldia magna ATCC 29328 for Genetic Mapping and Comparative Genomics

“…Finegoldia magna ATCC 29328 strain was purchased from the American Type Culture Collection and was grown anaerobically at 37 C in liquid or on agar plates using Gifu Anaerobic Medium (GAM) (Nissui) for [13][14][15][16][17][18][19][20][21][22][23][24] TM Electrocompetent E. coli (Epicentre), carrying recombinant pBeloBAC11, was cultured in LuriaBertani (LB) medium or on LB agar plates at 37 C for 24 hr. Where required, 12.5 µg/ml chloramphenicol (CM), 25 µg/ml isopropyl β-D-thiogalactopyranoside (IPTG) and 50 µg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) were added.…”

Description of Three New Species of the Genus Peptostreptococcus from Human Clinical Specimens: Peptostreptococcus harei sp. nov., Peptostreptococcus ivorii sp. nov., and Peptostreptococcus octavius sp. nov.

“…In addition to considerable metabolic diversity, members of the genus have been shown by nucleic acid analysis to be phylogenetically heterogeneous (2,11,15,22). In particular, Peptostreptococcus anaerobius, the type species of the genus Peptostreptococcus, is phylogenetically far removed from other peptostreptococci (11,22) and exhibits a close affinity with certain clostridial species (Clostridium rRNA cluster XI, as described by Collins et al [2]).…”

“…In addition to considerable metabolic diversity, members of the genus have been shown by nucleic acid analysis to be phylogenetically heterogeneous (2,11,15,22). In particular, Peptostreptococcus anaerobius, the type species of the genus Peptostreptococcus, is phylogenetically far removed from other peptostreptococci (11,22) and exhibits a close affinity with certain clostridial species (Clostridium rRNA cluster XI, as described by Collins et al [2]). Peptostreptococcusproductus has been shown to be a close phylogenetic relative of Clostridium coccoides and Streptococcus hansenii (Clostridium rRNA cluster XIVa [2]), whereas the majority of peptostreptococcal species form a phylogenetically distinct group closely related to Helcococcus kunzii (Clostridium rRNA cluster XI11 [2]).…”